Simultaneous detections of genetic fragment and single nucleotide mutation with a three-tiered output for tuberculosis diagnosis

Huang, Shih‐Yu; Chang, Jinghua Tsai; Liao, Yu-Chieh; Dou, Horng-Yunn; Chuang, Min‐Chieh

doi:10.1016/j.aca.2017.12.028

Cited by 3 publications

(2 citation statements)

References 41 publications

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“…1 The TB causative pathogen, Mycobacterium tuberculosis (M. tuberculosis), continuously produces strains harboring resistance genes, thus causing increasing challenge to TB treatment and prevention. 2 M. tuberculosis one of the leading causes of death from infectious diseases and is infected about 25% of the world's population. 3 Initially, TB attacks the lungs; however, other organs can be attacked by the pathogen, including the lymphatic system, blood circulation, central nervous system, and alimentary system.…”

Section: Introductionmentioning

confidence: 99%

GeneXpert MTB-RIF assay as a rapid and effective molecular method for the diagnosis of tuberculosis in Erbil City

Balaky

2019

Zanco J. Med. Sci.

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A total of 115 pulmonary and extrapulmonary samples were collected and diagnosed for tuberculosis detection. Acid fastbacilli, culture, and GeneXpert methods were used for the diagnosis of tuberculosis. Results: Out of 115 tuberculosis specimens, 70 were acid fast bacilli smear positive, while 97 samples were positive for Mycobacterium tuberculosis by GeneXpert assay. The results indicated a higher sensitivity of GeneXpert assay compared to the acid fastbacillistaining method. The sensitivity of GeneXpert was 91.6%, specificity 50%; positive and negative predictive values were 89.7% and 55.6%, respectively. No significant differences were detected between the Gene Xpert test results and the culture results (P = 0.815), and the total agreement between the tests was 84.3%. Conclusion:We concluded that the GeneXpert test results is sensitive as culture results for both pulmonary and extrapulmonary specimens. Although culture remains the gold standard for laboratory confirmation of tuberculosis disease, GeneXpert should become standard practice for patients suspected of tuberculosis, and all clinicians and public health tuberculosis programs should have access to molecular testing for tuberculosis to shorten the time of diagnosis.

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Section: Introductionmentioning

confidence: 99%

GeneXpert MTB-RIF assay as a rapid and effective molecular method for the diagnosis of tuberculosis in Erbil City

Balaky

2019

Zanco J. Med. Sci.

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“…The development of rapid nucleic acid detection methods with high specificity and sensitivity can provide vast opportunities for biological and cancer studies. Complementary base pairing ensures the high specificity of nucleic acid hybridization, and based on this principle, many types of detection methods or probes have been developed, such as nucleic acid amplification technologies, 1–3 molecular beacons, 4–7 fluorescent probes based on energy transfer, 8–10 colorimetry, 11,12 surface plasmon resonance, 13 electrochemiluminescence, 14 quartz crystal microbalance 15 or the use of new nanostructures. 16 However, existing methods often suffer from an unsatisfactory limit of detection (LOD) and a long detection time if accompanied by complicated nucleic acid amplification.…”

Section: Introductionmentioning

confidence: 99%

Ultrasensitive detection of nucleic acids based on dually enhanced fluorescence polarization

Wang

Ren

You

et al. 2018

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Highly sensitive detection of nucleic acids is crucial for genomics, transcriptomics, and heterogeneity studies. Conventional fluorescence polarization and intensity-based assays for DNA/RNA measurements often suffer from a poor limit of detection and a long test period. A versatile nanoscale probe based on a competitive displacement assay and fluorescence polarization was proposed for the simple, fast and sensitive detection of nucleic acids. For the probe, a partially complementary double-stranded DNA (dsDNA) was used as a connector of gold nanoparticles (AuNPs) and Alexa fluor 488 dyes (Alexa488), leading to the nanometal surface energy transfer (NSET) between Alexa488 and AuNPs. Meanwhile, suppression of the fluorescence intensity caused a decrease in the effective concentration of the Alexa488, and an increase in the volume or mass prolonged the rotational relaxation time of the Alexa488, both of which increased polarization of the Alexa488 in an aqueous solution. After competitive displacement between the probe and the target strand, the decrease in the volume or mass of the Alexa488 and fluorescence recovery resulted in the decline of the fluorescence polarization of the Alexa488, which could be used to sensitively detect the target concentration. After optimization, the fluorescence polarization-based method achieved a pM level detection of single-stranded nucleic acids within 30 minutes.

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Polymerase-assisted fluorescence resonance energy transfer (FRET) assay for simultaneous detection of binary viral sequences

Sun

Lee

Chuang

2018

Analytica Chimica Acta

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Simultaneous detections of genetic fragment and single nucleotide mutation with a three-tiered output for tuberculosis diagnosis

Cited by 3 publications

References 41 publications

GeneXpert MTB-RIF assay as a rapid and effective molecular method for the diagnosis of tuberculosis in Erbil City

GeneXpert MTB-RIF assay as a rapid and effective molecular method for the diagnosis of tuberculosis in Erbil City

Ultrasensitive detection of nucleic acids based on dually enhanced fluorescence polarization

Polymerase-assisted fluorescence resonance energy transfer (FRET) assay for simultaneous detection of binary viral sequences

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