Simultaneous detection ofListeriaspp. andListeria monocytogenesby reverse hybridization with 16S–23S rRNA spacer probes

Rijpens, N.; Jannes, Geert; Asbroeck, M. van; Herman, Lieve; Rossau, R.

doi:10.1006/mcpr.1995.0065

Cited by 17 publications

(13 citation statements)

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“…The large SR sequences were ideal for designing such species‐specific oligonucleotide probes for the following reasons. First, we observed the lowest degree of identity between the large SR sequences, as observed for Listeria species [17]. Second, the most variable region between the large sequences is located in a single region, i.e.…”

Section: Discussionmentioning

confidence: 52%

Rapid species identification within two groups of closely related lactobacilli using PCR primers that target the 16S/23S rRNA spacer region

Berthier

Ehrlich

1998

FEMS Microbiology Letters

215

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A rapid and reliable PCR-based method for distinguishing closely related species within two groups of lactobacilli is described. Primers complementary to species-specific sequences in the 16S/23S rDNA spacer regions were designed after sequencing and sequence comparison of the spacer regions of 32 strains. The strains belong to two groups of closely related Lactobacillus species; one composed of Lactobacillus curvatus, Lactobacillus graminis and Lactobacillus sake, the other of Lactobacillus paraplantarum, Lactobacillus pentosus and Lactobacillus plantarum. PCR assays with the designed primers and subsequent agarose gel analysis of the amplified fragments allowed the same species identification as the DNA/DNA hybridization procedure.

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Section: Discussionmentioning

confidence: 52%

Rapid species identification within two groups of closely related lactobacilli using PCR primers that target the 16S/23S rRNA spacer region

Berthier

Ehrlich

1998

FEMS Microbiology Letters

215

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“…Although many studies have successfully used the rrs (16s) gene for typing and identification purposes, the gene is conserved throughout the Pasteurellaceae family (Dewhirst et al, 1992). We selected the region separating the rrs and rrl genes since this shows sequence variability and length polymorphism which should make it an ideal target for identifying eubacteria at the species level (Barry et al, 1991;Rossau et al, 1992;Rijpens et al, 1995;Gurtler & Stanisch, 1996).…”

Section: Discussionmentioning

confidence: 99%

The rrs (16S)-rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay

Rossau

Jannes

et al. 1998

Microbiology

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The intergenic spacer region between the rrs and rrl ribosomal RNA genes of Haemophilus ducreyi was analysed and the DNA sequence was used for the selection of specific PCR primers. A highly sensitive and specific heminested-PCR assay for the identification of H. ducreyi was developed. The assay showed a sensitivity of 96% on genital ulcer specimens from patients with clinically diagnosed chancroid, compared with a sensitivity of 56% for culture methods.These results indicate that this PCR assay has the potential to become an accurate and easy reference method for the detection of H. ducreyi.

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“…Hybridization probes targeting 16S rDNA, the listeriolysin or enterotoxin genes, iap, inlA, prfA, the 16S-23S rRNA spacer region, or genomic sequences related to the expression of surface antigens have been developed for the detection and identification of L. monocytogenes [47,83,84,154,164,242,320,339,426,434,443]. Detection of B. cereus with hybridization is mainly performed to confirm the results obtained with specific PCR [79, 367,437].…”

Section: Hybridizationmentioning

confidence: 99%

Methodologies for the characterization of microbes in industrial environments: a review

Maukonen¹,

Mättö²,

Wirtanen³

et al. 2003

Journal of Industrial Microbiology and Biotechnology

121

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There is growing interest in research and development to develop novel tools to study, detect, and characterize microbes and their communities in industrial environments. However, knowledge about their validity in practical industrial use is still scarce. This review describes the advantages and limitations of traditional and molecular methods used for biofilm and/or planktonic cell studies, especially those performed with Listeria monocytogenes, Bacillus cereus, and/or Clostridium perfringens. In addition, the review addresses the importance of isolating the microorganisms from the industrial environment and the possibilities and future prospects for exploiting the described methods in the industrial environment.

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Simultaneous detection ofListeriaspp. andListeria monocytogenesby reverse hybridization with 16S–23S rRNA spacer probes

Cited by 17 publications

References 0 publications

Rapid species identification within two groups of closely related lactobacilli using PCR primers that target the 16S/23S rRNA spacer region

Rapid species identification within two groups of closely related lactobacilli using PCR primers that target the 16S/23S rRNA spacer region

The rrs (16S)-rrl (23S) ribosomal intergenic spacer region as a target for the detection of Haemophilus ducreyi by a heminested-PCR assay

Methodologies for the characterization of microbes in industrial environments: a review

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