Simultaneous detection of viral and bacterial enteric pathogens using the Seeplex® Diarrhea ACE detection system

Coupland, Lindsay; McElarney, Iain; Meader, Emma; Cowley, K.; Alcock, L.; Naunton, J.; Gray, Jim

doi:10.1017/s0950268812002622

Cited by 34 publications

(27 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The xTAG GPP test identifies 9 bacterial, 3 viral, and 3 parasitic pathogens. Both assays can be completed in approximately 5 h. Compared to routine culture, EIA, and singleplex PCR methods, the Seeplex test was 100% sensitive for 6/9 targets but was only 84.2%, 50.0%, and 87.5% sensitive for Salmonella spp., Clostridium difficile, and rotavirus, respectively (15). The sensitivity of the xTAG GPP was Ͼ95% for all targets except Norovirus strain GII (92.5%), Salmonella spp.…”

Section: Discussionmentioning

confidence: 96%

“…Commercially available multiplexed molecular assays for the identification of enteric pathogens remain limited; however, assays, including the Seeplex Diarrhea-V ACE (Seegene, Seoul, South Korea), BD MAX enteric bacterial panel (BD, Sparks, MD), and xTAG GPP (Luminex, Austin, TX), have received mandatory conformity marking for products sold in the European Union (CE marking). Of these, only the xTAG GPP has also attained FDA clearance, and there are few published studies evaluating the performance of these multiplex tests (15)(16)(17).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Clinical Evaluation of a Real-Time PCR Assay for Identification of Salmonella, Shigella, Campylobacter (Campylobacter jejuni and C. coli), and Shiga Toxin-Producing Escherichia coli Isolates in Stool Specimens

et al. 2013

View full text Add to dashboard Cite

e Enteric illness affects millions of individuals annually in the United States and results in >50,000 hospitalizations. The rapid and accurate identification of bacterial pathogens associated with gastroenteritis can aid acute patient management decisions, including the use of antibiotic therapy and infection control. This study compared the ProGastro SSCS multiplex real-time PCR assay (Gen-Probe Prodesse, San Diego, CA) to culture for the identification of Campylobacter spp. (Campylobacter jejuni and Campylobacter coli), Salmonella spp., and Shigella spp. and to broth enrichment followed by an FDA-cleared enzyme immunoassay (EIA) for the identification of Shiga toxin-producing Escherichia coli (STEC) isolates in stool specimens. Stool samples submitted in preservatives for routine culture and EIA were prospectively enrolled and tested at four clinical centers. Discrepancies between the ProGastro SSCS assay and culture or EIA were resolved using bidirectional sequencing. The overall prevalence of the pathogens as detected by culture was 5.6% (1.8% Campylobacter, 1.8% Salmonella, 1.3% Shigella, and 0.8% STEC). When results based on the ProGastro SSCS assay and bidirectional sequencing were applied, the overall prevalence increased to 8.3% (2.3% Campylobacter, 2.6% Salmonella, 1.8% Shigella, and 1.6% STEC). Following resolution of the discrepant results, the sensitivity of the ProGastro SSCS assay was 100% for all pathogens, and the specificities ranged from 99.4% to 100%. The sensitivity of culture compared to sequence-confirmed ProGastro SSCS results ranged from 52.9% to 76.9%, with the specificities ranging from 99.9% to 100%. Overall, these results suggest that the ProGastro SSCS assay is highly sensitive and specific in a clinical setting.

show abstract

Section: Discussionmentioning

confidence: 96%

mentioning

confidence: 99%

Clinical Evaluation of a Real-Time PCR Assay for Identification of Salmonella, Shigella, Campylobacter (Campylobacter jejuni and C. coli), and Shiga Toxin-Producing Escherichia coli Isolates in Stool Specimens

et al. 2013

View full text Add to dashboard Cite

show abstract

“…There are two patient cohort based evaluation studies on Seeplex Diarrhea ACE assays. One study was by Coupland LJ et al to evaluate Seeplex Diarrhea ACE multiplex detection for four viruses and/or ten bacteria with 223 patients’ samples 72 . Comparing to conventional methods and Norovirus-specific RT-PCR, Seeplex Diarrhea ACE panels showed 100% positive concordance for Adenovirus, Norovirus, Campylobacter spp., Escherichia coli O157, Shigella spp.…”

Section: Current Enteric Multiplex Commercial Assaysmentioning

confidence: 99%

Multiplex Polymerase Chain Reaction Tests for Detection of Pathogens Associated with Gastroenteritis

Zhang

Morrison

Tang

2015

Clinics in Laboratory Medicine

148

View full text Add to dashboard Cite

Synopsis A wide range of enteric pathogens can cause infectious gastroenteritis. Conventional diagnostic algorithms including culture, biochemical identification, immunoassay and microscopic examination are time consuming and often lack sensitivity and specificity. Advances in molecular technology have as allowed its use as clinical diagnostic tools. Multiplex PCR based testing has made its way to gastroenterology diagnostic arena in recent years. In this article we present a review of recent laboratory developed multiplex PCR tests and current commercial multiplex gastrointestinal pathogen tests. We will focus on two FDA cleared commercial syndromic multiplex tests: Luminex xTAG GPP and Biofire FimArray GI test. These multiplex tests can detect and identify multiple enteric pathogens in one test and provide results within hours. Multiplex PCR tests have shown superior sensitivity to conventional methods for detection of most pathogens. The high negative predictive value of these multiplex tests has led to the suggestion that they be used as screening tools especially in outbreaks. Although the clinical utility and benefit of multiplex PCR test are to be further investigated, implementing these multiplex PCR tests in gastroenterology diagnostic algorithm has the potential to improve diagnosis of infectious gastroenteritis.

show abstract

“…Coupland et al [132] also used multiple PCR-CE(Seeplex ® ) to simultaneously detect four viruses and 10 bacteria associated with acute gastroenteritis because acute gastroenteritis involves bacterial, viral, and parasitical infections. Conventional diagnostic methods detected 98 pathogens in 96 samples while Seeplex ® detected 81 pathogens in 75 samples, with the sensitivity and specificity were respective 84.21%-100% (except C. difficile was 50%) and 96.23%-100%.…”

Section: Simultaneously Detecting Multiple Types Of Pathogensmentioning

confidence: 99%

Capillary electrophoresis based on nucleic acid detection for diagnosing human infectious disease

Lian

Zhao

2016

Clinical Chemistry and Laboratory Medicine (CCLM)

View full text Add to dashboard Cite

Rapid transmission, high morbidity, and mortality are the features of human infectious diseases caused by microorganisms, such as bacteria, fungi, and viruses. These diseases may lead within a short period of time to great personal and property losses, especially in regions where sanitation is poor. Thus, rapid diagnoses are vital for the prevention and therapeutic intervention of human infectious diseases. Several conventional methods are often used to diagnose infectious diseases, e.g. methods based on cultures or morphology, or biochemical tests based on metabonomics. Although traditional methods are considered gold standards and are used most frequently, they are laborious, time consuming, and tedious and cannot meet the demand for rapid diagnoses. Disease diagnosis using capillary electrophoresis methods has the advantages of high efficiency, high throughput, and high speed, and coupled with the different nucleic acid detection strategies overcomes the drawbacks of traditional identification methods, precluding many types of false positive and negative results. Therefore, this review focuses on the application of capillary electrophoresis based on nucleic detection to the diagnosis of human infectious diseases, and offers an introduction to the limitations, advantages, and future developments of this approach.

show abstract

Simultaneous detection of viral and bacterial enteric pathogens using the Seeplex® Diarrhea ACE detection system

Cited by 34 publications

References 33 publications

Clinical Evaluation of a Real-Time PCR Assay for Identification of Salmonella, Shigella, Campylobacter (Campylobacter jejuni and C. coli), and Shiga Toxin-Producing Escherichia coli Isolates in Stool Specimens

Clinical Evaluation of a Real-Time PCR Assay for Identification of Salmonella, Shigella, Campylobacter (Campylobacter jejuni and C. coli), and Shiga Toxin-Producing Escherichia coli Isolates in Stool Specimens

Multiplex Polymerase Chain Reaction Tests for Detection of Pathogens Associated with Gastroenteritis

Capillary electrophoresis based on nucleic acid detection for diagnosing human infectious disease

Contact Info

Product

Resources

About