Simultaneous detection of six food-borne pathogens by multiplex PCR with a GeXP analyzer

Zhou, Beili; Xiao, Jinwen; Liu, Shengfeng; Yang, Jun; Wang, Yu; Nie, Fuping; Zhou, Qing; Li, Yingguo; Zhao, Guohua

doi:10.1016/j.foodcont.2012.11.044

Cited by 35 publications

(26 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…DNA‐based detection methods such as PCR can quickly obtain test results and greatly shorten clinical outbreak time. In recent years, different PCR based detection methods for some food‐borne pathogens have been developed (Alarcon, Garcia‐Canas, Cifuentes, Gonzalez, & Aznar, ; Pierce et al., ; Souza et al., ; Zhou et al., ). Traditional simplex PCR methods cannot detect multiple pathogens at the same time.…”

Section: Resultsmentioning

confidence: 99%

A multiplex PCR assay with a common primer for the detection of eleven foodborne pathogens

Tao

Liu

Ding

et al. 2020

Journal of Food Science

View full text Add to dashboard Cite

Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time‐consuming and labor‐intensive. Multiplex PCR technology, which can detect multiple targets in a single tube, has been increasingly applied to microbial detection due to its high specificity, sensitivity, and fast response. This paper is to establish a multiplex PCR technology mediated by a common primer for the detection of these 11 common foodborne pathogens in order to achieve the goal of nondirectional screening for these 11 common foodborne pathogens. The specificity of the established CP‐MPCR detection system was first verified by 100 clinical isolates. The sensitivity of the CP‐MPCR detection system was then detected by using cultured bacteria preparations and has been confirmed with a high sensitivity of 103 to 104 CFU/mL, among them, the sensitivity of the CP‐MPCR for Vibrio cholerae and S. flexneri can even achieve 102 CFU/mL. Sixty anal swab samples collected from Suzhou CDC and 16 enrichment cultured solutions of food samples collected from the Suzhou Food Inspection and Testing Center were tested using the CP‐MPCR system. A total of 32 positive results were detected. Practical Application Food poisoning incidents occur frequently around the world, mainly because of the contamination of food by pathogenic bacteria and serious harm to human health. The method provided in this study can detect 11 foodborne pathogens in food, which can effectively prevent the spread of pathogenic microorganisms. At the same time, for the food poisoning incident that has already occurred, this method can be used for diagnosis to find out the cause.

show abstract

Section: Resultsmentioning

confidence: 99%

A multiplex PCR assay with a common primer for the detection of eleven foodborne pathogens

Tao

Liu

Ding

et al. 2020

Journal of Food Science

View full text Add to dashboard Cite

show abstract

“…and Shigella spp. (Cheah et al, 2008 ; Lee et al, 2008 ; Alves et al, 2012 ; Chiang et al, 2012 ; Zhou et al, 2013 ).…”

Section: Nucleic Acid-based Methodsunclassified

Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations

et al. 2015

View full text Add to dashboard Cite

The incidence of foodborne diseases has increased over the years and resulted in major public health problem globally. Foodborne pathogens can be found in various foods and it is important to detect foodborne pathogens to provide safe food supply and to prevent foodborne diseases. The conventional methods used to detect foodborne pathogen are time consuming and laborious. Hence, a variety of methods have been developed for rapid detection of foodborne pathogens as it is required in many food analyses. Rapid detection methods can be categorized into nucleic acid-based, biosensor-based and immunological-based methods. This review emphasizes on the principles and application of recent rapid methods for the detection of foodborne bacterial pathogens. Detection methods included are simple polymerase chain reaction (PCR), multiplex PCR, real-time PCR, nucleic acid sequence-based amplification (NASBA), loop-mediated isothermal amplification (LAMP) and oligonucleotide DNA microarray which classified as nucleic acid-based methods; optical, electrochemical and mass-based biosensors which classified as biosensor-based methods; enzyme-linked immunosorbent assay (ELISA) and lateral flow immunoassay which classified as immunological-based methods. In general, rapid detection methods are generally time-efficient, sensitive, specific and labor-saving. The developments of rapid detection methods are vital in prevention and treatment of foodborne diseases.

show abstract

“…Thus, rapid, sensitive, and high‐throughput methods are needed. Therefore, Zhou and others () developed a GeXP‐MPCR (MPCR‐CE‐LIF) assay to simultaneously detect 6 food‐borne pathogens ( Salmonella enterica , Shigella spp ., L. monocytogenes , C. jejuni , S. aureus , and E. coli O157:H7 ). The specificity of the assay was 100%, and its sensitivity was 10 1 to 10 2 CFU/mL.…”

Section: Procedures For Food Analysis Based On Ce‐based Nucleic Acid Dmentioning

confidence: 99%

Capillary Electrophoresis Based on Nucleic Acid Detection as Used in Food Analysis

Lian

Zeng

2017

Comp Rev Food Sci Food Safe

View full text Add to dashboard Cite

Food safety and food production are closely related to the health of consumers. Food-related accidents often cause tremendous losses of personnel and property. Thus, rapid detection and analysis of ingredients in food, tracing food sources, studying the optimal conditions for food production, and more are vital for preventing incidents related to safety. Conventional analysis based on proteomics, microbial cultures, and morphology, as well as biochemical tests based on metabonomics, are considered gold standards and used frequently, but they are labor-intensive, time-consuming, tedious, error-prone, and incapable of meeting the demand for rapid and precise detection at a large scale. Alternative detection methods that utilize capillary electrophoresis have the advantages of high efficiency, high throughput, high speed, and automation; these methods are coupled with various nucleic acid detection strategies to overcome the drawbacks of traditional identification methods, and to prevent false results. Therefore, this review focuses on the application of capillary electrophoresis based on nucleic acid detection in food analysis and provides an introduction to the limitations, advantages, and future developments of this approach.

show abstract

Simultaneous detection of six food-borne pathogens by multiplex PCR with a GeXP analyzer

Cited by 35 publications

References 19 publications

A multiplex PCR assay with a common primer for the detection of eleven foodborne pathogens

A multiplex PCR assay with a common primer for the detection of eleven foodborne pathogens

Rapid methods for the detection of foodborne bacterial pathogens: principles, applications, advantages and limitations

Capillary Electrophoresis Based on Nucleic Acid Detection as Used in Food Analysis

Contact Info

Product

Resources

About