Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector

Xu, Jinzhong; Shen, Jie; Cheng, Yiyu; Qu, Haibin

doi:10.1631/jzus.b0820095

Cited by 51 publications

(32 citation statements)

References 13 publications

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“…However, Danshen injection has been shown to vary considerably depending on different pretreatment and manufacture processes [16][17][18] . Furthermore, the diverse geographical origins and climates of the plants cause the content of active components to differ significantly [19,20] .…”

Section: Wwwchinapharcom Chang Bb Et Almentioning

confidence: 99%

“…Furthermore, the diverse geographical origins and climates of the plants cause the content of active components to differ significantly [19,20] . The content variation of Sal A or Sal B is easily observed in different batches of Danshen injections, sometimes more than tenfold [17,21] . This variation was also reflected in a recent fingerprint study [14] .…”

Section: Wwwchinapharcom Chang Bb Et Almentioning

confidence: 99%

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Pharmacokinetic interactions induced by content variation of major water-soluble components of Danshen preparation in rats

et al. 2010

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Aim: To investigate the pharmacokinetic interactions induced by content variation of the main water-soluble components of Danshen injection in rats. Methods: Intravenous Danshen injection (control) or Danshen injection with danshensu (DSS), protocatechuic aldehyde (PAL), salvianolic acid A (Sal A) or salvianolic acid B (Sal B) were administered to female Sprague Dawley rats . Plasma concentrations of DSS, Sal A, PAL and its oxidative metabolite protocatechuic acid (PA) were analyzed simultaneously with LC-MS/MS; concentrations of Sal B were determined by the LC-MS method. Non-compartmental pharmacokinetic parameters were calculated and compared for identifying the pharmacokinetic interactions among these components. Results: Compared with the control group, the DSS, Sal A, and Sal B groups had significant increases in AUC 0-∞ in response to elevated concentrations of PAL ( by 78.1%, 51.0%, and 82.9%, respectively), while the clearances (CL) were markedly reduced (by 42.5%, 32.9%, and 46.8%, respectively). Similarly, Sal A increased the AUC 0-∞ of DSS and Sal B (26.7% and 82.4%, respectively) and substantially decreased their clearances (21.4% and 45.6%, respectively). In addition, the pharmacokinetics of DSS and Sal B were significantly affected by the content variation of the other major components; the AUC 0-∞ increased by 45.1% and 52.1%, respectively, the CL dropped by 29.6% and 27.1%, respectively, and the T 1/2 was decreased by 22.0% and 19.6%, respectively. Conclusion: Complex, extensive pharmacokinetic interactions were observed among the major water-soluble constituents in the Danshen injection. The content variation of PAL had the most significant effect on the pharmacokinetic behaviors of other major constituents. Furthermore, the pharmacokinetics of DSS and Sal B were the most susceptible to the content change of other components.

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Section: Wwwchinapharcom Chang Bb Et Almentioning

confidence: 99%

Section: Wwwchinapharcom Chang Bb Et Almentioning

confidence: 99%

Pharmacokinetic interactions induced by content variation of major water-soluble components of Danshen preparation in rats

et al. 2010

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“…For in vitro study, the DSI was diluted in Dulbecco's modified Eagle's medium (DMEM) and sonicated. The main components in the extract is as follows (Xu et al, 2008 …”

Section: Methodsmentioning

confidence: 99%

Evaluation of cardiomyocyte hypoxia injury models for the pharmacological studyin vitro

Zhu¹,

Deng²

2011

Pharmaceutical Biology

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“…[9][10][11][12][13] A study of RA variation in sage collection describes sample preparation by solid-liquid extraction using 50% aqueous methanol with sonication, followed by analysis using a C 18 column and elution with mixtures of acetic acid, water, methanol and acetonitrile. 14 However, no special attention has been given to the evaluation and prevention of possible RA auto-oxidation during the extraction procedure.…”

Section: Introductionmentioning

confidence: 99%

HPLC/DAD determination of rosmarinic acid in Salvia officinalis: sample preparation optimization by factorial design

Oliveira¹,

Oliveira²

2013

J. Braz. Chem. Soc.

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A sálvia (Salvia officinalis) contém altos teores de ácido rosmarínico (RA), uma substância bioativa, bem como outros polifenóis. RA é facilmente oxidável e pode sofrer degradação durante a preparação da amostra para análise. O objetivo deste trabalho foi desenvolver e validar um método analítico para a determinação do RA em sálvia, usando planejamento fatorial para a otimização da preparação de amostras. Inicialmente foram determinadas as variáveis estatisticamente significativas para melhorar o rendimento da extração do RA, as quais foram depois otimizadas usando planejamento composto central (CCD). O método analítico foi validado e aplicado na análise de amostras comerciais de sálvia. O procedimento otimizado consistiu na extração com metanol aquoso (40%) contendo uma mistura antioxidante (ácido ascórbico e ácido etileno diamino tetracético (EDTA)), com sonicação a 45 o C por 20 min. As amostras foram injetadas em um sistema contendo coluna C 18 , usando metanol (A) e ácido fosfórico aquoso 0.1% (B) em gradiente (45A:55B, 0-5 min; 80A:20B, 5-10 min) com fluxo de 1.0 mL min −1 e deteção em 330 nm. Utilizando estas condições, as concentrações do RA foram 50% maiores quando comparadas com extração na ausência de antioxidante (recuperação de 98,94 ± 1,07%). Auto-oxidação do RA durante a preparação da amostra foi evitada pelo uso de antioxidante levando a resultados analíticos mais confiáveis. O método foi então aplicado na análise de amostras comerciais de sálvia.Sage (Salvia officinalis) contains high amounts of the biologically active rosmarinic acid (RA) and other polyphenolic compounds. RA is easily oxidized, and may undergo degradation during sample preparation for analysis. The objective of this work was to develop and validate an analytical procedure for determination of RA in sage, using factorial design of experiments for optimizing sample preparation. The statistically significant variables for improving RA extraction yield were determined initially and then used in the optimization step, using central composite design (CCD). The analytical method was then fully validated, and used for the analysis of commercial samples of sage. The optimized procedure involved extraction with aqueous methanol (40%) containing an antioxidant mixture (ascorbic acid and ethylenediaminetetraacetic acid (EDTA)), with sonication at 45 o C for 20 min. The samples were then injected in a system containing a C 18 column, using methanol (A) and 0.1% phosphoric acid in water (B) in step gradient mode (45A:55B, 0-5 min; 80A:20B, 5-10 min) with flow rate of 1.0 mL min −1 and detection at 330 nm. Using this conditions, RA concentrations were 50% higher when compared to extractions without antioxidants (98.94 ± 1.07% recovery). Auto-oxidation of RA during sample extraction was prevented by the use of antioxidants resulting in more reliable analytical results. The method was then used for the analysis of commercial samples of sage.

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Simultaneous detection of seven phenolic acids in Danshen injection using HPLC with ultraviolet detector

Cited by 51 publications

References 13 publications

Pharmacokinetic interactions induced by content variation of major water-soluble components of Danshen preparation in rats

Pharmacokinetic interactions induced by content variation of major water-soluble components of Danshen preparation in rats

Evaluation of cardiomyocyte hypoxia injury models for the pharmacological studyin vitro

HPLC/DAD determination of rosmarinic acid in Salvia officinalis: sample preparation optimization by factorial design

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