Simultaneous detection of reduced and oxidized forms of coenzyme Q10 in human cerebral spinal fluid as a potential marker of oxidative stress

Nagase, Midori; Yamamoto, Yorihiro; Mitsui, Jun; Tsuji, Shoji

doi:10.3164/jcbn.17-131

Cited by 9 publications

(7 citation statements)

References 14 publications

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“…It is noteworthy that only the oxidized form was detected in both nonirradiated and irradiated eyeball samples in the current study. This might be due to the oxidation of CoQ10H2 during the lipid extraction and irradiation process since CoQ10H2 is known to be sensitive to oxidation 32 .…”

Section: Discussionmentioning

confidence: 99%

Coenzyme Q10 in the eye isomerizes by sunlight irradiation

Mamun

Nabi

Sato

et al. 2022

Sci Rep

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Photoisomerization of lipids has been well studied. As for the eyes, photoisomerization from 11-cis isomer to all-trans-retinal is well-known as the first step of the visual transduction in the photoreceptors. In addition to that, there would be other ocular lipids that undergo photoisomerization, which may be involved in ocular health and function. To explore any photoisomerizable lipids in the eyes, the nonirradiated and sunlight-irradiated eyeball extracts were subjected to liquid chromatography-mass spectrometry analysis, followed by the identification of the decreased lipid species in the irradiated extracts. Surprisingly, more than nine hundred lipid species were decreased in the irradiated extracts. Three lipid species, coenzyme Q10 (CoQ10), triglyceride(58:4), and coenzyme Q9, were decreased both significantly (p < 0.05) and by more than two-fold, where CoQ10 showed the most significant decrease. Later, photoisomerization was identified as the prominent cause underlying the decrease of CoQ10. Interestingly, CoQ10 in the sunlight-irradiated fresh eyeballs was also isomerized. Both the visible light and ultraviolet radiation were capable of producing CoQ10 isomer, while the latter showed rapid action. This study is believed to enhance our understanding of the biochemistry and photodamage of the eye and can potentially contribute to the advancement of opto-lipidomics.

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Section: Discussionmentioning

confidence: 99%

Coenzyme Q10 in the eye isomerizes by sunlight irradiation

Mamun

Nabi

Sato

et al. 2022

Sci Rep

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“…As reported previously, CoQ and FC levels in cells were analyzed using HPLC-UV, ECD. (21,(24)(25)(26) Briefly, cells were collected in isopropanol, centrifuged, and the resulting supernatant was analyzed HPLC. Two separation columns (Ascentis ® C8, 5 μm, 250 mm × 4.6 mm i.d.…”

Section: Methodsmentioning

confidence: 99%

Transferrin, insulin, and progesterone modulate intracellular concentrations of coenzyme Q and cholesterol, products of the mevalonate pathway, in undifferentiated PC12 cells

Nakamura

Aida

Okamoto

et al. 2023

J. Clin. Biochem. Nutr.

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Coenzyme Q (CoQ) is important not only as an essential lipid for the mitochondrial electron transport system, but also as an antioxidant. CoQ levels decrease during aging and in various diseases. Orally administered CoQ is not readily taken up in the brain, so it is necessary to develop a method to increase the amount of CoQ in neurons. CoQ is synthesized via mevalonate pathway, like cholesterol. Transferrin, insulin, and progesterone are factors used in the culture of neurons. In this study, we determined the effect of these reagents on cellular CoQ and cholesterol levels. The administration of transferrin, insulin, and progesterone increased cellular CoQ levels in undifferentiated PC12 cells. When serum was removed and only insulin was administered, intracellular CoQ levels increased. This increase was even more pronounced with concurrent administration of transferrin, insulin, and progesterone. Cholesterol level decreased by the administration of transferrin, insulin, and progesterone. Progesterone treatment lowered intracellular cholesterol levels in a concentration-dependent manner. Our findings suggest that transferrin, insulin, and progesterone may be useful in regulating CoQ levels and cholesterol levels, which are products of the mevalonate pathway.

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“…Concentrations of CoQ and FC in cells were determined using HPLC, as reported previously, with minor modifications.‍ ( 22 ) Briefly, cells collected with 2-propanol were centrifuged, and thus, the obtained supernatant was injected into the HPLC system. Two separation columns (Ascentis‍ ® C8, 5 μm, 250 mm × 4.6 mm i.d.…”

Section: Methodsmentioning

confidence: 99%

Cellular level of coenzyme Q increases with neuronal differentiation, playing an important role in neural elongations

Nakamura

Okamoto

Maeda

et al. 2022

J. Clin. Biochem. Nutr.

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Deficiency of coenzyme Q has been reported in various neurological diseases, and the behavior of this lipid in neurons has attracted attention. However, the behavior of this lipid in normal neurons remains unclear. In this study, we analyzed the concentration of coenzyme Q before and after neuronal differentiation. Nerve growth factor treatment of PC12 cells caused neurite outgrowth and neuronal differentiation, and the amount of intracellular coenzyme Q increased dramatically during this process. In addition, when the serum was removed from the culture medium of N1E-115 cells and the neurite outgrowth was confirmed, the intracellular coenzyme Q level also increased. To elucidate the role of the increased coenzyme Q, we administered nerve growth factor to PC12 cells with coenzyme Q synthesis inhibitors and found that coenzyme Q levels decreased, neurite outgrowth was impaired, and differentiation markers were reduced. These results indicate that coenzyme Q levels increase during neuronal differentiation and that this increase is important for neurite outgrowth.

show abstract

Simultaneous detection of reduced and oxidized forms of coenzyme Q10 in human cerebral spinal fluid as a potential marker of oxidative stress

Cited by 9 publications

References 14 publications

Coenzyme Q10 in the eye isomerizes by sunlight irradiation

Coenzyme Q10 in the eye isomerizes by sunlight irradiation

Transferrin, insulin, and progesterone modulate intracellular concentrations of coenzyme Q and cholesterol, products of the mevalonate pathway, in undifferentiated PC12 cells

Cellular level of coenzyme Q increases with neuronal differentiation, playing an important role in neural elongations

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