Simultaneous detection of multiple lower genital tract pathogens by an impedimetric immunochip

Bianco

Nigro

et al. 2018

Sensors

Self Cite

125

Interest in extracellular vesicles and in particular microvesicles and exosomes, which are constitutively produced by cells, is on the rise for their huge potential as biomarkers in a high number of disorders and pathologies as they are considered as carriers of information among cells, as well as being responsible for the spreading of diseases. Current methods of analysis of microvesicles and exosomes do not fulfill the requirements for their in-depth investigation and the complete exploitation of their diagnostic and prognostic value. Lab-on-chip methods have the potential and capabilities to bridge this gap and the technology is mature enough to provide all the necessary steps for a completely automated analysis of extracellular vesicles in body fluids. In this paper we provide an overview of the biological role of extracellular vesicles, standard biochemical methods of analysis and their limits, and a survey of lab-on-chip methods that are able to meet the needs of a deeper exploitation of these biological entities to drive their use in common clinical practice.

Section: Exploring the Content Of Vesiclesmentioning

confidence: 99%

Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization

Bianco

Nigro

et al. 2018

Sensors

Self Cite

125

“…The platform is suitable for quick and low-cost screening of infections in the time of a gynaecological examination. This way, patients can leave the doctor’s office with a targeted antibiotic prescription, start the pharmacological treatment immediately, based on a real test and not only on symptoms, and without recurring to costly and time-consuming traditional assays like culture medium tests [ 62 ].…”

Section: Poc Tools For Personalized Medicinementioning

confidence: 99%

Key Enabling Technologies for Point-of-Care Diagnostics

Primiceri

Notarangelo

et al. 2018

Sensors

Self Cite

A major trend in biomedical engineering is the development of reliable, self-contained point-of-care (POC) devices for diagnostics and in-field assays. The new generation of such platforms increasingly addresses the clinical and environmental needs. Moreover, they are becoming more and more integrated with everyday objects, such as smartphones, and their spread among unskilled common people, has the power to improve the quality of life, both in the developed world and in low-resource settings. The future success of these tools will depend on the integration of the relevant key enabling technologies on an industrial scale (microfluidics with microelectronics, highly sensitive detection methods and low-cost materials for easy-to-use tools). Here, recent advances and perspectives will be reviewed across the large spectrum of their applications.

“…Among novel approaches, Lab-on-a-Chip (LOC) devices are of increasing interest in many fields of application from clinical diagnostics 16 – 18 to agro-food monitoring 19 , 20 and are showing potential applications in plant pathology, thanks to low cost, rapidity and sensitivity 21 , 22 , and may represents an intriguing grower-friendly method for plant pathogen monitoring 23 . Recently, an In-Check system was also tested for quarantine pests such as Citrus tristeza virus (CTV), showing as LOC devices may be comparable with capillary electrophoresis single-strand conformation polymorphism (CE-SSCP) analysis and multiple molecular marker (MMM) assays 24 .…”

Section: Introductionmentioning

confidence: 99%

Development of a lab-on-a-chip method for rapid assay of Xylella fastidiosa subsp. pauca strain CoDiRO

Luvisi

Primiceri

et al. 2018

Sci Rep

Self Cite

Xylella fastidiosa subsp. pauca strain CoDiRO, a pathogen responsible for Olive Quick Decline Syndrome (OQDS), is strongly threatening the agricultural-based economy of South Italy and making its typical landscape collapse. The bacteria can also infect more than other twenty woody or shrub species and quarantine programs are carried out in Italy. Since symptoms of OQDS like leaf scorching and wilting of canopy may appear several months after infection and some hosts are asymptomatic, a tool for the rapid and early screening of plants is desirable, in order to plan a sudden control strategy and apply programs for pest management. X. fastidiosa detection is usually performed by ELISA and PCR methods. In this work, the two standard methods are compared with an innovative on-chip detection strategy for X. fastidiosa assay from leaves samples, based on an electrochemical transduction method. The realized lab-on-chip includes also a microfluidic module and its performances are competitive with conventional diagnostic methods in terms of reliability, but with further advantages of portability, low-costs and ease of use. Thus, the proposed technology has the potential to provide a useful assay method for large-scale monitoring programs.