Simultaneous Detection of <b><i>Mycobacterium tuberculosis</i></b> and Atypical Mycobacteria by DNA-Microarray in Egypt

Abstract

Background and Objectives: Immunocompromised patients are considered a high-risk group to develop mycobacterial infections with either pulmonary and/or extra-pulmonary diseases. Low-cost and low-density (LCD) DNA-microarray is considered an easy and efficient method to detect typical and atypical Mycobacterial species. Materials and methods: Thirty immunocompromised patients were recruited to provide their clinical specimens (sputum, serum, urine and lymph node aspirate). Both Real-Time PCR and LCD-microarray techniques were performed and compared to the conventional methods of Ziehl-Neelsen (ZN) staining and Lowenstein Jensen (LJ) culturing. Results and conclusion: Mycobacterium tuberculosis complex (MTBC) was detected in all 30 clinical specimens (100% sensitivity) by Real-Time PCR and LCD-microarray. Additionally, co-infection with 4 atypical species belonging to mycobacteria other than tuberculosis (MOTT) was identified in 7 sputum specimens. These atypical mycobacterial species were presented as M. kansasii 10% (n=3), M. avium complex 6.6% (n=2), M. gordanae 3.3% (n=1) and M. peregnium 3.3% (n=1). This study documents the presence of certain species of atypical mycobacteria among immunocompromised patients in Egypt. To the best of our knowledge, this is the first detection of M. peregenium among clinical specimen in Egypt.

DNA microarray chip assay in new use: early diagnostic value in cutaneous mycobacterial infection

“…DNA microarray chip technology has emerged as a promising tool for the rapid detection of mycobacterial species ( Tobler et al., 2006 ; Gaballah et al., 2022 ). Zhu et al.…”

“…DNA microarray chip technology has emerged as a promising tool for the rapid detection of mycobacterial species (Tobler et al, 2006;Gaballah et al, 2022). Zhu et al established a DNA microarray platform based on the principle of polymorphism in the 16S rRNA space region for multi-target, rapid, and simultaneous detection of 17 pathogenic mycobacteria associated with pulmonary mycobacterial infection, including NTM and MTB (Zhu et al, 2010;Shi et al, 2012).…”