Simultaneous Detection of Local Polarizability and Viscosity by a Single Fluorescent Probe in Cells

Abbandonato, Gerardo; Polli, Dario; Viola, Daniele; Cerullo, Giulio; Storti, Barbara; Cardarelli, Francesco; Salomone, Fabrizio; Nifosı̀, Riccardo; Signore, Giovanni; Bizzarri, Ranieri

doi:10.1016/j.bpj.2018.02.032

Cited by 8 publications

(6 citation statements)

References 43 publications

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“…With probe 75 , the low temperature caused viscosity increase was visualized. The Bizzarri group presented a green fluorescent protein chromophore analog 76 for quantifying and imaging polarity and viscosity [105] . Yan et al.…”

Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Xiao

Tang

2021

Chemistry A European J

109

115

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Viscosity, as a vital microenvironment parameter, is tightly associated with multitudinous cellular processes and diseases. Recently, precise visualization of viscosity has started to arouse more and more interest. However, owing to the complicated character, it is still a huge challenge to directly observe viscosity in living systems. In this regard, mounting fluorescence probes are being increasingly fabricated to map viscosity inside live cells and small animals. In this minireview, the viscosity‐sensitive small molecular fluorescent probes used in bioimaging are comprehensively summarized, mainly focusing on the last three years. Moreover, the current challenges and opportunities for the development of viscosity‐specific fluorescent probes will be discussed.

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Section: Small Molecular Fluorescent Probes For Viscositymentioning

confidence: 99%

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Xiao

Tang

2021

Chemistry A European J

109

115

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“…Recently, we demonstrated how a close derivative of the green fluorescent protein (GFP) chromophore, Ge1, acts as a dual probe of polarity and viscosity, providing fully decoupled fluorescence responses to these parameters (29). In this work, we demonstrate that a lipid bioconjugate of Ge1, Ge1L (Fig.…”

Section: Introductionmentioning

confidence: 73%

“…Conversely, the fluorescence emission ranges between 497 and 521 nm (Table 1), depending on the dielectric properties of the solvent (38). The fluorescence decay of Ge1L is biexponential (Table 1), in keeping with the peculiar emission photophysics of its fluorogenic unit Ge1 that entails two concurring excited states (29). Yet, time-resolved anisotropy measurements in tetrahydrofurane (THF) show a rotational correlation time (q) of $200 ps, much longer than expected for untargeted Ge1 (1-10 ps).…”

Section: Spectroscopic Properties Of Ge1l In Solution and In Luvsmentioning

confidence: 87%

Lipid-Conjugated Rigidochromic Probe Discloses Membrane Alteration in Model Cells of Krabbe Disease

Abbandonato

Storti

Tonazzini

et al. 2019

Biophysical Journal

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The plasma membrane of cells has a complex architecture based on the bidimensional liquid-crystalline bilayer arrangement of phospho-and sphingolipids, which in turn embeds several proteins and is connected to the cytoskeleton. Several studies highlight the spatial membrane organization into more ordered (L o or lipid raft) and more disordered (L d ) domains. We here report on a fluorescent analog of the green fluorescent protein chromophore that, when conjugated to a phospholipid, enables the quantification of the L o and L d domains in living cells on account of its large fluorescence lifetime variation in the two phases. The domain composition is straightforwardly obtained by the phasor approach to confocal fluorescence lifetime imaging, a graphical method that does not require global fitting of the fluorescence decay in every spatial position of the sample. Our imaging strategy was applied to recover the domain composition in human oligodendrocytes at rest and under treatment with galactosylsphingosine (psychosine). Exogenous psychosine administration recapitulates many of the molecular fingerprints of a severe neurological disease, globoid cell leukodystrophy, better known as Krabbe disease. We found out that psychosine progressively destabilizes plasma membrane, as witnessed by a shrinking of the L o fraction. The unchanged levels of galactosyl ceramidase, i.e., the enzyme lacking in Krabbe disease, upon psychosine treatment suggest that psychosine alters the plasma membrane structure by direct physical effect, as also recently demonstrated in model membranes.

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“…In addition to temperature as an obvious physical parameter for biomedical applications, there are several other elements to be examined in the biophysics field. For instance, several indicators were reported to visualize invisible factors, such as viscosity (Battisti et al, 2013; Liu et al, 2014), molecular crowding (Boersma et al, 2015), polarity (Sunahara et al, 2007; Abbandonato et al, 2018), and tension (Grashoff et al, 2010). Although the importance of these factors for biomaterials development still remains vague, these options should be kept in mind.…”

Section: Toolsets To Visualize Intracellular Events In Real-timementioning

confidence: 99%

The ABC Guide to Fluorescent Toolsets for the Development of Future Biomaterials

Ferdinandus

Arai

2019

Front. Bioeng. Biotechnol.

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In recent decades, diversified approaches using nanoparticles or nano-structured scaffolds have been applied to drug delivery and tissue engineering. Thanks to recent interdisciplinary studies, the materials developed have been intensively evaluated at animal level. Despite these efforts, less attention has been paid to what is really going on at the subcellular level during the interaction between a nanomaterial and a cell. As the proposed concept becomes more complex, the need for investigation of the dynamics of these materials at the cellular level becomes more prominent. For a deeper understanding of cellular events, fluorescent imaging techniques have been a powerful means whereby spatiotemporal information related to cellular events can be visualized as detectable fluorescent signals. To date, several excellent review papers have summarized the use of fluorescent imaging toolsets in cellular biology. However, applying these toolsets becomes a laborious process for those who are not familiar with imaging studies to engage with owing to the skills gap between them and cell biologists. This review aims to highlight the valuable essentials of fluorescent imaging as a tool for the development of effective biomaterials by introducing some cases including photothermal and photodynamic therapies. This distilled information will be a convenient short-cut for those who are keen to fabricate next generation biomaterials.

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Simultaneous Detection of Local Polarizability and Viscosity by a Single Fluorescent Probe in Cells

Cited by 8 publications

References 43 publications

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Small Molecular Fluorescent Probes for Imaging of Viscosity in Living Biosystems

Lipid-Conjugated Rigidochromic Probe Discloses Membrane Alteration in Model Cells of Krabbe Disease

The ABC Guide to Fluorescent Toolsets for the Development of Future Biomaterials

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