Simultaneous Detection of Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola in Rice Seed Using a Padlock Probe-Based Assay

Tian, Yuee; Zhao, Yuqiang; Xu, Rui; Liu, Fengquan; Hu, Baishi; Walcott, R. R.

doi:10.1094/phyto-10-13-0274-r

Cited by 20 publications

(23 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Compared with compounds (1-3), all hydroxyls were substituted by methoxyls on the benzene rings in compounds (7)(8)(9). Interestingly, compounds (7-9) had no inhibitory effects on Xoo growth, indicating that the hydroxyls were the vital active group for the inhibitory effects on Xoo growth observed for compounds (1)(2)(3). For many inhibitors, the double bond and the hydroxyls played important roles in the anti-Xoo activity, such as sphaeropsidin A, the main phytotoxin produced by Diplodia cupressi [17], and it was reported that stilbenoids with at least one free hydroxyl group as a common structural feature suggested an association with the antimicrobial activity, such as against Bacillus subtilis and Pseudomonas syringae [18].…”

Section: Inhibitory Effects On Growth Of Xoo and The Potential Structmentioning

confidence: 96%

“…Dramatically, resveratrol (1) showed the strongest antibacterial activity against Xoo (IC 50 11.67 ± 0.58 μg/mL) ( Table 2), and at 5 μg/mL, 25 μg/mL, and 100 μg/mL, the inhibiting percentage on Xoo growth was 24.66 ± 1.79, 75.84 ± 3.14, and 90.49 ± 0.28, respectively. For compounds (2)(3)(4)(5)(6), the inhibiting percentages on Xoo growth at 100 μg/mL were 89.39 ± 0.43, 78.89 ± 0.80, 82.76 ± 1.02, 54.30 ± 6.05, and 58.51 ± 3.11, respectively, and at 5 μg/mL were 16…”

Section: Antibacterial Activity Of Compounds (1-9) Against Xoomentioning

confidence: 99%

“…The chemical structures of three stilbenoids (1)(2)(3) and their derivatives (4)(5)(6)(7)(8)(9), di-hydro-resveratrol (4), di-hydro-oxyresveratrol (5), di-hydro-piceatannol (6), tri-methyl-resveratrol (7), tetra-methyloxyresveratrol (8), and tetra-methyl-piceatannol (9), were shown in Fig. 1.…”

Section: Structural Identification Of Three Stilbenoids (1-3) and Dermentioning

confidence: 99%

“…MICs of compounds (1-9) were determined by following the two-fold dilution method [36]. In brief, overnight cultures of Xoo (1%, v/v) were resuspended in fresh nutrient agar (NB) medium in the presence of the samples (0.001-0.1 mg/mL for compounds (1-6) and 0.01-1 mg/mL for compounds (7-9)) in 96-well plates, then incubated at 28 °C and 180 rpm for 18 h. The MICs of compounds (1)(2)(3)(4)(5)(6)(7)(8)(9) against Xoo are shown in Table 1.…”

Section: Minimum Inhibitory Concentrations (Mic)mentioning

confidence: 99%

See 3 more Smart Citations

Resveratrol inhibits the growth of Xanthomonas oryzae pv. oryzae and the metabolomic analysis

Luo

Guan

et al. 2019

Preprint

View full text Add to dashboard Cite

Background Xanthomonas oryzae pv. oryzae ( Xoo ) can cause destructive bacterial leaf blight in rice. As an antibacterial, resveratrol may inhibit Xoo growth. This study focused on the potential structural-activity relationship of resveratrol and its derivatives against Xoo growth, and 1 H-NMR-based metabolomic analysis was applied to investigate the global metabolite changes in Xoo after resveratrol treatment.Results Resveratrol showed the strongest inhibitory effects on Xoo growth compared with its derivatives, which lacked double bonds (compounds 4 – 6 ) or hydroxyls were substituted with methoxyls (compounds 7 – 9 ). The IC 50 of resveratrol against Xoo growth was 11.67 ± 0.58 μg/mL. Results indicated that the double bond of resveratrol contributed to its inhibitory effects on Xoo growth, and hydroxyls were vital for this inhibition. Interestingly, resveratrol also significantly inhibited Xoo flagellum growth. Based on 1 H-NMR global metabolic analysis, a total of 30 Xoo metabolites were identified, the changes in the metabolic profile indicated that resveratrol could cause oxidative stress as well as disturb energy, purine, amino acid, and NAD + metabolism in Xoo , resulting in the observed inhibitory effects on growth.Conclusions This study showed that the double bond of resveratrol contributed to its inhibitory effects on Xoo growth, and hydroxyls were also the important active groups. Resveratrol could cause oxidative stress of Xoo cells, and disturb the metabolism of energy, purine, amino acid and NAD +, thus inhibit Xoo growth.

show abstract

Section: Inhibitory Effects On Growth Of Xoo and The Potential Structmentioning

confidence: 96%

Section: Antibacterial Activity Of Compounds (1-9) Against Xoomentioning

confidence: 99%

Section: Structural Identification Of Three Stilbenoids (1-3) and Dermentioning

confidence: 99%

Section: Minimum Inhibitory Concentrations (Mic)mentioning

confidence: 99%

See 2 more Smart Citations

Resveratrol inhibits the growth of Xanthomonas oryzae pv. oryzae and the metabolomic analysis

Luo

Guan

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…This process termed as the padlock probe assay has been used in the detection of many plant diseases. [87][88][89] High multiplexity and specificity of padlock probes followed by RCA also increases the popularity of its application in multiplex detection of plant pathogens. In addition, RCA has been reported to have higher specificity and be less prone to nonspecific amplification than PCR.…”

Section: Rcamentioning

confidence: 99%

Development of point-of-care and multiplex diagnostic methods for the detection of plant pathogens

Lau¹

View full text Add to dashboard Cite

New diagnostic technologies for the detection of plant pathogens with high multiplexing ability and point-of-care (POC) capability are an essential tool in the fight to reduce the large agricultural production losses caused by plant diseases. The main desirable characteristics for such diagnostic assays are high specificity, sensitivity, reproducibility, quickness, cost efficiency and highthroughput multiplex detection capability. The aim of this project is to develop new methods combining nucleic acid amplification, multiplexing and point-of-care application for the detection of plant pathogens.Isothermal amplification methods, such as recombinase polymerase amplification (RPA) which operates at low constant temperature are especially suited for POC applications. However, the development of a rapid and simplified detection method for the isothermal amplification products in resource-poor settings is still challenging. A new method to visualize the success of the amplification step, and therefore the presence of pathogen DNA in a sample, was developed based on bridging flocculation. This method requires minimal equipment and can be assessed by the naked eye allowing its use in POC settings. The method was initially applied to the rapid and sensitive detection of several important plant pathogens and subsequently extended to animal and human pathogens to demonstrate the wide applications of the approach. A nanoparticle based electrochemical biosensor was also developed for rapid and sensitive detection of amplified plant pathogen DNA on screen-printed carbon electrodes. Gold nanoparticles were employed as a probe for electrochemical assessment by differential pulse voltammetry (DPV). This method was 10,000 times more sensitive than conventional polymerase chain reaction (PCR)/gel electrophoresis and could readily identify P. syringae infected plant samples even before the disease symptoms were visible.To allow multiplex detection of plant pathogen, a novel method that can screen for thousands of plant pathogens with high specificity and sensitivity using molecular inversion probes (MIPs) was been developed. As proof of concept, a MIP targeting a unique DNA sequence present in the F.oxysporum f.sp. conglutinans (Foc) genome was designed. The specificity, sensitivity and detection limit of the assay were assessed and used to detect the presence of pathogen in infected Arabidopsis thaliana samples. This methodology successfully detected as little as 2.5 ng of pathogen DNA and it was highly specific, being able to accurately differentiate Fusarium oxysporum f.sp. conglutinans from other fungal pathogens such as Botrytis cinerea and even pathogens of the same species such as Fusarium oxysporum f.sp. lycopersici. Finally, a diagnostic platform for POC plant pathogen detection using a combination of surface-enhanced Raman ii scattering (SERS) and RPA was developed for rapid multiplex detection. The RPA-SERS method was faster, more sensitive than polymerase chain reaction and could detect as little as 2 copies of...

show abstract

Detection and Identification of Bacterial and Phytoplasmal Pathogens

2017

Microbial Plant Pathogens

View full text Add to dashboard Cite

Simultaneous Detection of Xanthomonas oryzae pv. oryzae and X. oryzae pv. oryzicola in Rice Seed Using a Padlock Probe-Based Assay

Cited by 20 publications

References 31 publications

Resveratrol inhibits the growth of Xanthomonas oryzae pv. oryzae and the metabolomic analysis

Resveratrol inhibits the growth of Xanthomonas oryzae pv. oryzae and the metabolomic analysis

Development of point-of-care and multiplex diagnostic methods for the detection of plant pathogens

Detection and Identification of Bacterial and Phytoplasmal Pathogens

Contact Info

Product

Resources

About