Simultaneous Detection of Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes at a Very Low Level Using Simultaneous Enrichment Broth and Multichannel SPR Biosensor

Zhang, Xiaoguang; Tsuji, Sachiko; Kitaoka, Hayato; Kobayashi, Hiroshi; Tamai, Mitsuru; Honjoh, Ken Ichi; Miyamoto, Takahisa

doi:10.1111/1750-3841.13843

Cited by 32 publications

(20 citation statements)

References 32 publications

(44 reference statements)

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“…Recently, Zhang et al developed a multichannel SPR‐based biosensor for detection of E. coli O157:H7 simultaneously with other two vital food‐borne pathogens: L. monocytogenes and S. enteritidis . The biosensor was able to detect 14, 28, and 6 CFU/mL of E. coli O157:H7, L. monocytogenes , and S. enteritidis , respectively .…”

Section: Medical Applications Of Biosensorsmentioning

confidence: 99%

Biosensors: Classifications, medical applications, and future prospective

Alhadrami

2017

Biotech and App Biochem

117

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Biosensors are devices that combine a biological material with a suitable platform for detection of pathogenic organisms, carcinogenic, mutagenic, and/or toxic chemicals or for reporting a biological effect. In recent years, an enormous number of different types of biosensors have been constructed and developed for several medical applications. The reason for that was primarily due to the numerous advantages and applications that can be offered by biosensors. This review article has been started with demonstrating the power of biosensor technologies versus analytical and conventional techniques. Subsequently, more emphasis has been added on the classification and the role of biosensors in several medical applications such as detection and monitoring of carcinogenic and mutagenic chemicals, reporting of endocrine disrupting compounds, and detection of pathogenic organisms. The most common reporter genes used in biosensors engineering and construction have also been summarized. Prospective strategies and recommendations for the future construction of biosensors have been highlighted.

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Section: Medical Applications Of Biosensorsmentioning

confidence: 99%

Biosensors: Classifications, medical applications, and future prospective

Alhadrami

2017

Biotech and App Biochem

117

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“…Rapid pathogenic bacterial diagnosis has been applied to conduct measurements in biological and food matrix [3]. Up to date, different method has been applied by several research group for enumeration of pathogenic bacteriaespecially L. monocytogenes using polymerase chain reaction immunoassay [4,5], electrochemical sensors [6][7][8], bioluminescence [9,10], DNA-based sensors [11,12], ELISA [13,14], surface plasmon resonance [15,16], fluorescence [17,18], surface-enhanced Raman scattering (SERS) [19][20][21]. It was indicated that the reported methods were optimized to select proper system usage to obtain selectivity and precision, there were some problems such as poor sensitivity and long experimental procedures.…”

Section: Introductionmentioning

confidence: 99%

Immunomagnetic separation and Listeriamonocytogenes detection with surface-enhanced Raman scattering

Akçinar¹,

Aslım²,

Torul³

et al. 2020

Turk J Med Sci

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Background/aim: We aimed to develop a rapid method to enumerate Listeria monocytogenes (L. monocytogenes) utilizing magnetic nanoparticle based preconcentration and surface-enhanced Raman spectroscopy measurements. Materials and methods: Biological activities of magnetic Au-nanoparticles have been observed to have the high biocompatibility, and a sample immunosensor model has been designed to use avidin attached Au-nanoparticles for L. monocytogenes detection. Staphylococcus aureus (S. aureus) and Salmonella typhimurium (S. typhimurium) bacteria cultures were chosen for control studies. Antimicrobial activity studies have been done to identify bio-compatibility and bio-characterization of the Au-nanoparticles in our previous study and capturing efficiencies to bacterial surfaces have been also investigated. Results: We constructed the calibration graphs in various population density of L. monocytogenes as 2.2 × 10 1 to 2.2 × 10 6 cfu/mL and the capture efficiency was found to be 75%. After the optimization procedures, population density of L. monocytogenes and Raman signal intensity showed a good linear correlation (R 2 = 0.991) between 10 2 to 10 6 cfu/mL L. monocytogenes. The presented sandwich assay provides low detection limits and limit of quantification as 12 cfu/mL and 37 cfu/mL, respectively. We also compared the experimental results with reference plate-counting methods and the practical utility of the proposed assay is demonstrated using milk samples. Conclusion: It is focused on the enumeration of L. monocytogenes in milk samples and the comparision of results of milk analysis obtained by the proposed SERS method and by plate counting method stay in food agreement. In the present study, all parameters were optimized to select SERS-based immunoassay method for L. monocytogenes bacteria to ensure LOD, selectivity, precision and repeatablity.

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“…; and result in high economic losses and considerable mortality of contaminated consumers, such as Escherichia coli. Thus, identifying these microorganisms is important to evaluate the quality of food and prevent food outbreaks (ZHANG et al, 2017). In this context, meat products require especial care because of their potential to carry foodborne pathogens, especially when undergo intense handling (SHAWISH; TARABEES, 2017).…”

Section: Introductionmentioning

confidence: 99%

Microbiological characterization of vacuum-packed and conventionally packed sliced turkey hams marketed in Mossoró, Brazil

et al. 2019

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Article history The consumption of turkey ham has been increasing considerably. This product can be marketed sliced, and thus, it is subjected to intense manipulation and presents a high microbial load, which compromises its quality and safety. The objective of this study was to characterize microbiologically vacuum-packed and conventionally packed sliced turkey hams marketed in Mossoró, state of Rio Grande do Norte, Brazil. The analyses consisted of mold and yeast, viable aerobic mesophilic microorganism, and coagulase positive Staphylococcus counts, determination of the most probable number of total coliforms and thermotolerant coliforms, and presence of Salmonella spp. Turkey hams present high microbiological counts regardless of their packaging, but conformity for coliforms at 45 °C. Some samples had low quality, denoting that a greater care in the manipulation and conservation of this product is necessary to ensure the safety and absence of risks of this product to public health.

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Simultaneous Detection of Escherichia coli O157:H7, Salmonella enteritidis, and Listeria monocytogenes at a Very Low Level Using Simultaneous Enrichment Broth and Multichannel SPR Biosensor

Abstract: Our method is expected as a rapid and simultaneous detection method for pathogens at very low levels. It has great potential for safety control of food and microbiological detection applications.

Cited by 32 publications

References 32 publications

Biosensors: Classifications, medical applications, and future prospective

Biosensors: Classifications, medical applications, and future prospective

Immunomagnetic separation and Listeriamonocytogenes detection with surface-enhanced Raman scattering

Microbiological characterization of vacuum-packed and conventionally packed sliced turkey hams marketed in Mossoró, Brazil

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