Simultaneous detection of hydrogen and methane in breath for the diagnosis of small intestinal bacterial overgrowth by fast gas chromatography

Wang, Min; Zhang, Xusheng; Zhang, Junyu; Xue, Yingying; Wan, Hao; Wang, Ping

doi:10.1039/c8ay01451e

Cited by 10 publications

(5 citation statements)

References 25 publications

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“…The first probes were natural carbohydrates that could be metabolized into inorganic and very volatile compounds (e.g. H 2 , CH 4 and CO 2 ) [63][64][65]. They were then succeeded by synthetic probes, among which a myriad of isotopic CO 2 -based probes were designed for diagnosis purposes [66].…”

Section: Contribution Of Induced-volatolomics To Chemical Biologymentioning

confidence: 99%

Induced-volatolomics, a new research field in chemical biology

Blochouse,

Eid,

Cannoni

et al. 2024

Comptes Rendus. Chimie

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Progresses in chemical biology have substantially deepened our understanding of biological pathways related to disease outcomes. Numerous optical probes activatable by disease-relevant enzymes have been designed and have shown great value for imaging crucial biochemical transformations specific to pathological processes. In comparison to their "always-on" counterparts, such stimuli-responsive probes stand out as promising candidates for exploring biological processes because of their high sensitivity, ingenious spatiotemporal resolution, ease of operation, and real-time and in situ imaging capacity. However, these imaging tools meet several bottlenecks related to either stability, background noise, limited penetration depth or systemic toxicity, pharmacokinetics, tissue distribution, and renal clearance.In parallel, novel volatile organic compound (VOC)-based probes have been proposed under the emerging paradigm of induced-volatolomics. These probes are converted into exogenous VOCs in response to pathogen or eukaryote specific enzymatic stimuli. Once activated, VOC-based probes release volatile tracers that inform the biochemical processes arising in cells, liquid and solid biopsies, and even in entire organisms. After a brief presentation of recent developments in the field of induced-volatolomics, we discuss possible improvements of this new modality for the exploration of biological systems.

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Section: Contribution Of Induced-volatolomics To Chemical Biologymentioning

confidence: 99%

Induced-volatolomics, a new research field in chemical biology

Blochouse,

Eid,

Cannoni

et al. 2024

Comptes Rendus. Chimie

View full text Add to dashboard Cite

show abstract

“…In these cases, complementary CH 4 detection in breath can enhance the HBT and, therefore, might improve the correct diagnosis of malabsorption issues [ 194 , 195 ]. Hydrogen-methane breath testing has been hitherto underutilised due to the lack of low-cost, easy-to-operate instruments, but recent technical advances have led to the emergence of commercial devices [ 194 ], such as the BreathTracker TM SC analyser by QuinTron Instrument Company, Inc. (Milwaukee, WI, USA), which separates the compounds in an alveolar gas sample using a fast GC combined with a solid-state sensor and is used to identify carbohydrate malabsorption and small intestinal bacterial overgrowth (SIBO) sufferers [ 196 ]. Other devices target multiple components by incorporating electrochemical H 2 sensors and IR sensors for CH 4 and CO 2 detection (Lactotest 202 by Medical Electrionic Construction, Nivelles, Belgium) [ 197 ], or IR sensors for the simultaneous measurement of H 2 , CH 4 and O 2 , with additional electrochemical sensors for CH 4 and H 2 detection (GastroCH 4 ECK by Bedfont Scientific Ltd., Maidstone, UK) [ 195 ].…”

Section: Tests Exploiting Exogenous Compoundsmentioning

confidence: 99%

Breath Biomarkers in Diagnostic Applications

Pham

Beauchamp

2021

Molecules

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The detection of chemical compounds in exhaled human breath presents an opportunity to determine physiological state, diagnose disease or assess environmental exposure. Recent advancements in metabolomics research have led to improved capabilities to explore human metabolic profiles in breath. Despite some notable challenges in sampling and analysis, exhaled breath represents a desirable medium for metabolomics applications, foremost due to its non-invasive, convenient and practically limitless availability. Several breath-based tests that target either endogenous or exogenous gas-phase compounds are currently established and are in practical and/or clinical use. This review outlines the concept of breath analysis in the context of these unique tests and their applications. The respective breath biomarkers targeted in each test are discussed in relation to their physiological production in the human body and the development and implementation of the associated tests. The paper concludes with a brief insight into prospective tests and an outlook of the future direction of breath research.

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“…Carbonyl sulphide (OCS) is related to liver diseases [24]. Methane (CH 4 ) is linked to colonic fermentation and intestinal problem [25][26][27], while ethane (C 2 H 6 ) is an indicator of oxidative damage within the organism resulting from, e.g. asthma or COPD [28,29].…”

Section: Introductionmentioning

confidence: 99%

Exhaled breath analysis using cavity-enhanced optical techniques: a review

Banik¹,

Mizaikoff²

2020

J. Breath Res.

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Cavity-enhanced absorption spectroscopies (CEAS) have gained importance in a wide range of applications in molecular spectroscopy. The development of optical sensors based on the CEAS techniques coupled with the continuous wave or pulsed laser sources operating in the mid-infrared or near-infrared spectral regime uniquely offers molecularly selective and ultra-sensitive detection of trace species in complex matrices including exhaled human breath. In this review, we discussed recent applications of CEAS for analyzing trace constituents within the exhaled breath matrix facilitating the non-invasive assessment of human health status. Next to a brief discussion on the mechanisms of formation of trace components found in the exhaled breath matrix related to particular disease states, existing challenges in CEAS and future development towards non-invasive clinical diagnostics will be discussed.

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Simultaneous detection of hydrogen and methane in breath for the diagnosis of small intestinal bacterial overgrowth by fast gas chromatography

Abstract: A convenient, small-sized and multi-functional fast gas chromatograph (Fast GC) with a low detection limit and sufficient resolution to simultaneously determine hydrogen and methane concentrations in breath is presented.

Cited by 10 publications

References 25 publications

Induced-volatolomics, a new research field in chemical biology

Induced-volatolomics, a new research field in chemical biology

Breath Biomarkers in Diagnostic Applications

Exhaled breath analysis using cavity-enhanced optical techniques: a review

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