Simultaneous detection of green tea catechins and gallic acid in human serum after ingestion of green tea tablets using ion-pair high-performance liquid chromatography with electrochemical detection

Narumi, Keiko; Sonoda, Junichiro; Shiotani, Keita; Shigeru, Michihiro; Shibata, Masayuki; Kawachi, Asuka; Tomishige, Erisa; Sato, Keizo; Motoya, Toshiro

doi:10.1016/j.jchromb.2013.11.007

Cited by 74 publications

(54 citation statements)

References 18 publications

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“…Additionally, GA can also be extracted from many herbs and fruits, such as Rheum officinale , Eucalyptus robusta Smith , Semen corni , and pomegranate husk. However, due to the complicacy of these natural matrixes and the high polarity of GA, some interferences are often co‐eluted through traditional elution methods, including chromatographic methods, SPE, LLE, and accelerated solvent extraction . Therefore, it is necessary to develop a simple technology to selectively separate and enrich GA from complex natural resources.…”

Section: Introductionmentioning

confidence: 99%

Highly selective and efficient imprinted polymers based on carboxyl‐functionalized magnetic nanoparticles for the extraction of gallic acid from pomegranate rind

Zhang

Yue

et al. 2017

J of Separation Science

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With the combined surface imprinting technique and immobilized template strategy, molecularly imprinted magnetic nanoparticles were successfully prepared and coupled with high-performance liquid chromatography to selectively separate and determine gallic acid from the pomegranate rind. On the surface of carboxyl-functionalized magnetic nanospheres, thin imprinting shells were formed using dopamine as monomer and crosslinker. The characteristics, polymerization conditions, and adsorption performances of the resultant nanomaterials were investigated in detail. In addition of good crystallinity, satisfactory magnetism, and uniform morphology of the obtained polymers, they had rapid binding kinetics, high adsorption capacity, and favorable reusability. In the mixed solution of four hydroxybenzoic acids, the prepared nanomaterials have an excellent selectivity to gallic acid with an imprinting factor of as high as 17.5. Therefore, the polymers have great potentials in specific extraction and enrichment of gallic acid from the complex natural resources.

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Section: Introductionmentioning

confidence: 99%

Highly selective and efficient imprinted polymers based on carboxyl‐functionalized magnetic nanoparticles for the extraction of gallic acid from pomegranate rind

Zhang

Yue

et al. 2017

J of Separation Science

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“…To assess the pharmacokinetics of green tea catechins after ingestion, we developed a new analytical method for detecting green tea catechins in serum. Pharmacokinetic study using this method showed individual variation of catechin serum concentration after ingestion of same amount of green tea catechins [4]. This observation indicated the existence of unknown factors affecting the pharmacokinetics of green tea catechins.…”

Section: Introductionmentioning

confidence: 74%

“…All the analytes (4 catechins and GA, and Ethyl gallate asan internal standard) were separated and obtained as single peaks, and the accuracy, reproducibility, and sensitivity of this method were sufficient to measure the target catechins and GA in serum [4].…”

Section: Methods Of Analysis For Green Tea Catechinsmentioning

confidence: 99%

“…On the other hand, Suzuki et al reported in a pooled analysis of two prospective studies with 35,000 Japanese women that there was no association between consumption of green tea and risk of breast cancer [33]. Because catechin absorption from the intestines to the bloodstream is relatively low [4], a high dose of about10 cups/d in addition to along duration of green tea consumption maybe necessary to achieve an inverse association between green tea consumption and cancer incidence. Although inconsistent results have been observed in a case-controlled study and a prospective study, Li et al showed that there was a statistically significant 19% reduction of breast cancer incidence among woman with high green tea intake in the most recent meta-analysis of two cohort studies and five casecontrolled studies [34][35][36][37][38][39][40][41].…”

Section: Prostate Cancermentioning

confidence: 99%

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Green Tea Catechins -Pharmacokinetic Properties and Health Beneficial Effects

ErisaTomishige¹

2015

Pharm Anal Acta

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In this review, we provide information on thepharmacokinetic properties of green tea catechins and their beneficial health effects. The major catechins in green tea are (-)-epicatechin (EC), its hydroxyl derivative (-)-epigallocatechin (EGC), and their gallic acid esters, (-)-epicatechin-3-gallate (ECg) and (-)-epigallocatechin-3-gallate (EGCg). We developed an analytical method for determination of the presence of green tea catechins in human serum using ion-pair HPLC with electrochemical detection to estimate the pharmacokinetic parameters of target catechins. The C max values indicated that catechin absorption was relatively low. One of the gallatedcatechins, EGCg, had a longer half-life than the non-gallatedcatechins. Green tea catechins, in particular, have attracted attention as cancer preventive agents in terms of their low toxicity and being readily available to the general population. Several epidemiological studies revealed that green tea consumption reduces cancer incidence. Numerous in vitro cell culture studies have shown that EGCg, which is defined as a major green tea catechin contributing to green tea's anticancer effects, inhibits cell growth concomitant with induction of apoptosis. We have previously found that the cell death-inhibiting gene, Bcl-xL, was decreased by EGCg. These results support the hypothesis that EGCg regulates cytoplasmic NF-κB and subsequently induction of apoptosis. Green tea consumption may also play a role in preventing other lifestyle diseases, such as cardiovascular diseases and stroke, due to its hypocholesterolemic and hypotensive activities. In conclusion, habitual green tea drinking may promote human health by preventing lifestyle-related diseases.

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“…For detection mode, an electrochemical detection offers high potential of interest. They can be used as a detector in the UHPLC system for the determination of varietal electroactive antioxidants [12][13][14]. Various electrode materials have been also applied to detect these electroactive polyphenolic compounds such as metal electrode (platinum electrode [15]), glassy carbon electrode (GCE) [16,17], carbon-paste electrode (CPE) [18] and screen-printed carbon electrode (SPCE) [19].…”

Section: Introductionmentioning

confidence: 99%

Ultra-high performance liquid chromatographic determination of antioxidants in teas using inkjet-printed graphene–polyaniline electrode

Bardpho

Rattanarat

Siangproh

et al. 2016

Talanta

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Simultaneous detection of green tea catechins and gallic acid in human serum after ingestion of green tea tablets using ion-pair high-performance liquid chromatography with electrochemical detection

Cited by 74 publications

References 18 publications

Highly selective and efficient imprinted polymers based on carboxyl‐functionalized magnetic nanoparticles for the extraction of gallic acid from pomegranate rind

Highly selective and efficient imprinted polymers based on carboxyl‐functionalized magnetic nanoparticles for the extraction of gallic acid from pomegranate rind

Green Tea Catechins -Pharmacokinetic Properties and Health Beneficial Effects

Ultra-high performance liquid chromatographic determination of antioxidants in teas using inkjet-printed graphene–polyaniline electrode

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