Simultaneous detection of exosomal microRNAs by nucleic acid functionalized disposable paper-based sensors

Yang, Huisi; Zhao, Jichun; Dong, Jiangbo; Wen, Li; Hu, Zhikun; He, Congjuan; Xu, Fen; Huo, Dehong; Hou, Changjun

doi:10.1016/j.cej.2022.135594

Cited by 28 publications

(11 citation statements)

References 38 publications

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“…[35] By introducing nanomotors probes, particles capable of selfmotion upon NIR irradiation, the authors increase the capture efficiency of the target sequence of about 30 % within a simple, dual-mode LFA. It should be noted that, even if the literature is characterized by plenty of sensing architectures to be applied for miRNA sensing within the cancer field, [71][72][73][74] the definition of PoC is strictly related to its efficacy close to/or near the patient.…”

Section: Poc Testing For Mirnas Detectionmentioning

confidence: 99%

Point‐of‐Care Testing for the Detection of MicroRNAs: Towards Liquid Biopsy on a Chip

Moro,

Fratte,

Normanno

et al. 2023

Angew Chem Int Ed

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Point‐of‐care (PoC) testing is revolutionizing the healthcare sector improving patient care in daily hospital practice and allowing reaching even remote geographical areas. In the frame of cancer management, the design and validation of PoC enabling the non‐invasive, rapid detection of cancer markers is urgently required to implement liquid biopsy in clinical practice. Therefore, focusing on stable blood‐based markers with high‐specificity, such as microRNAs, is of crucial importance. In this work, we highlight the potential impact of circulating microRNAs detection on cancer management and the development of PoC testing devices, especially for low‐income countries. A detailed discussion about the challenges that should be faced to promote the technological transfer and clinical use of these tools has been added, to provide the readers with a complete overview of potentialities and current limitations.

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Section: Poc Testing For Mirnas Detectionmentioning

confidence: 99%

Point‐of‐Care Testing for the Detection of MicroRNAs: Towards Liquid Biopsy on a Chip

Moro,

Fratte,

Normanno

et al. 2023

Angew Chem Int Ed

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“…It detected significantly higher expression of exosomal miR-21 in 500 μL plasma samples from breast cancer patients ( n = 10) than normal controls ( n = 10). Zhang et al 22 and Yang et al 23 developed DNA strand displacement reaction (SDR)-based electrochemical sensors to detect exosomal microRNAs. In the electrochemical biosensor developed by Zhang et al , 22 as shown in Figure 3, the DNA walkers first partially hybridized with the capture probes (L-Cp) immobilized on magnetic beads to form DNA walker-L-Cp-magnetic bead complexes.…”

Section: Nanotechnology-enabled Biosensors For Exosomal Rna Detectionmentioning

confidence: 99%

“…The biosensor detected 2.5-fold higher levels of exosomal miR-21 in serum samples from breast cancer patients than normal controls, demonstrating its potential clinical applications in breast cancer diagnosis. In the electrochemical sensor developed by Yang et al , 23 the electrode was prepared by depositing gold nanoparticles on the graphene-coated conductive carbon fiber paper, which provided a large, three-dimensional, and highly sensitive sensing surface. Hairpin DNA capture probes were conjugated on the surface of the electrode to capture microRNA targets.…”

Section: Nanotechnology-enabled Biosensors For Exosomal Rna Detectionmentioning

confidence: 99%

Recent advances in nanotechnology-enabled biosensors for detection of exosomes as new cancer liquid biopsy

Hsu

2022

Exp Biol Med (Maywood)

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Cancer liquid biopsy detects circulating biomarkers in body fluids, provides information that complements medical imaging and tissue biopsy, allows sequential monitoring of cancer development, and, therefore, has shown great promise in cancer screening, diagnosis, and prognosis. Exosomes (also known as small extracellular vesicles) are cell-secreted, nanosized vesicles that transport biomolecules such as proteins and RNAs for intercellular communication. Exosomes are actively involved in cancer development and progression and have become promising circulating biomarkers for cancer liquid biopsy. Conventional exosome characterization methods such as quantitative reverse transcription polymerase chain reaction (qRT-PCR) and enzyme-linked immunosorbent assay (ELISA) are limited by low sensitivity, tedious process, large sample volume, and high cost. To overcome these challenges, new biosensors have been developed to offer sensitive, simple, fast, high throughput, low sample consumption, and cost-effective detection of exosomal biomarkers. In this review, we summarized recent advances in nanotechnology-enabled biosensors that detect exosomal RNAs (both microRNAs and mRNAs) and proteins for cancer screening, diagnosis, and prognosis. The biosensors were grouped based on their sensing mechanisms, including fluorescence-based biosensors, colorimetric biosensors, electrical/electrochemical biosensors, plasmonics-based biosensors, surface-enhanced Raman spectroscopy (SERS)-based biosensors, and inductively coupled plasma mass spectrometry (ICP-MS) and photothermal biosensors. The future directions for the development of exosome-based biosensors were discussed.

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“…It has a high accuracy for exosomal miRNA determination, but this technique suffers from drawbacks such as long assay time, tedious operation process, and low sensitivity. At present, many analysis methods have been exploited to detect exosomal miRNAs, such as fluorescence, electrochemiluminescence, surface-enhanced Raman scattering, photo-electrochemical sensing, and electrochemistry. − Among them, electrochemical approaches are attractive due to the excellent merits of low cost, fast response, outstanding portability, and easy manipulation . Inspiringly, the emergence of a homogeneous electrochemical strategy has opened up a promising avenue owing to its immobilization-free property.…”

Section: Introductionmentioning

confidence: 99%

Integrating Reliable Pt–S Bond-Mediated 3D DNA Nanomachine with Magnetic Separation in a Homogeneous Electrochemical Strategy for Exosomal MicroRNA Detection with Low Background and High Sensitivity

Yang,

Guo,

Gao

et al. 2023

Anal. Chem.

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Simultaneous detection of exosomal microRNAs by nucleic acid functionalized disposable paper-based sensors

Cited by 28 publications

References 38 publications

Point‐of‐Care Testing for the Detection of MicroRNAs: Towards Liquid Biopsy on a Chip

Point‐of‐Care Testing for the Detection of MicroRNAs: Towards Liquid Biopsy on a Chip

Recent advances in nanotechnology-enabled biosensors for detection of exosomes as new cancer liquid biopsy

Integrating Reliable Pt–S Bond-Mediated 3D DNA Nanomachine with Magnetic Separation in a Homogeneous Electrochemical Strategy for Exosomal MicroRNA Detection with Low Background and High Sensitivity

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