Simultaneous detection of cell-secreted TNF-α and IFN-γ using micropatterned aptamer-modified electrodes

Liu, Ying; Kwa, Timothy; Revzin, Alexander

doi:10.1016/j.biomaterials.2012.06.089

Cited by 137 publications

(119 citation statements)

References 42 publications

(36 reference statements)

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“…Tumor necrosis factor-alpha (TNF-α) is a critical cytokine, which have been correlated to many infectious and inflammatory diseases such as rheumatoid arthritis, diabetes, stroke, HIV infection, neonatal listeriosis, systemic erythema nodosum leprosum, endotoxic shock, severe meningococcemia and graft rejection (Chu, 2013;de Kossodo et al, 1995;Liu et al, 2012;Sun et al, 2013). Generally, the concentration of biomarkers in human fluids such as in blood, urine and other tissues is very low, and therefore, the development of sensitive methods for the detection of biomarkers is of great importance because it helps in early diagnosis of diseases, and also provides the possibility of better treatment of diseases.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and electrocatalytic effect of Ag@Pt core–shell nanoparticles supported on reduced graphene oxide for sensitive and simple label-free electrochemical aptasensor

Mazloum-Ardakani

Hosseinzadeh

Taleat

2015

Biosensors and Bioelectronics

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Section: Introductionmentioning

confidence: 99%

Synthesis and electrocatalytic effect of Ag@Pt core–shell nanoparticles supported on reduced graphene oxide for sensitive and simple label-free electrochemical aptasensor

Mazloum-Ardakani

Hosseinzadeh

Taleat

2015

Biosensors and Bioelectronics

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“…Moreover, no cross-reaction between the two aptamers towards non-cognate proteins was reported. Liu et al [15] described an electrochemical aptasensor array for the detection of interferon gamma (IFN-γ) and tumour necrosis factor alpha (TNF-α), using micropatterned aptamer-modified gold electrodes. The developed aptasensor array showed similar sensitivity to that of the single aptasensors for both proteins, and it was found to be applicable for the detection of those proteins in activated T cells and monocytes cell lines.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Aptasensor Array for Multiple Detection of Human Osteopontin

Meirinho

Dias

Peres

et al. 2018

Port. Electrochim. Acta

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Electrochemical aptasensor arrays have been recently used to detect several proteins reported as disease biomarkers, mainly due to their known advantages, that include high affinity, sensitivity, specificity and low costs, among others. This study describes the development of a label-free electrochemical multi-aptasensor array, for the simultaneous detection of human osteopontin (OPN), using two specific aptamers. To enable multiplexed protein assay, RNA and DNA aptamers were immobilized in the dual-screen-printed gold working electrodes via streptavidin-biotin interaction, and using the [Fe(CN)6] 3−/4− as the redox probe for cyclic voltammetry measurements. The multi-aptasensor array herein developed exhibited a good response and selectivity to detect human OPN in the presence of other interfering proteins. Considering these preliminary results, the DNA/RNA dual aptasensor array could potentially be used as an analytical tool for the specific detection of human OPN, and for cancer diagnosis overall.

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“…Several groups including our own have been using microfabrication platforms for detecting protein release from immune cells. These have included microwells or microengravings, [10][11][12] microring resonators, 13 aptasensors 14,15 and antibody arrays. [16][17][18] Of particular note is the recent report by Heath lab describing the method of loading T-cells into microfluidic devices and then segmenting microfluidic channels to create pL chambers for detecting cytokine release from single cells.…”

Section: Introductionmentioning

confidence: 99%

Reconfigurable microfluidics combined with antibody microarrays for enhanced detection of T-cell secreted cytokines

Chen

Stybayeva

et al. 2013

Biomicrofluidics

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Cytokines are small proteins secreted by leukocytes in blood in response to infections, thus offering valuable diagnostic information. Given that the same cytokines may be produced by different leukocyte subsets in blood, it is beneficial to connect production of cytokines to specific cell types. In this paper, we describe integration of antibody (Ab) microarrays into a microfluidic device to enable enhanced cytokine detection. The Ab arrays contain spots specific to cell-surface antigens as well as anti-cytokine detection spots. Infusion of blood into a microfluidic device results in the capture of specific leukocytes (CD4 T-cells) and is followed by detection of secreted cytokines on the neighboring Ab spots using sandwich immunoassay. The enhancement of cytokine signal comes from leveraging the concept of reconfigurable microfluidics. A three layer polydimethylsiloxane microfluidic device is fabricated so as to contain six microchambers (1 mm × 1 mm × 30 μm) in the ceiling of the device. Once the T-cell capture is complete, the device is reconfigured by withdrawing liquid from the channel, causing the chambers to collapse onto Ab arrays and enclose cell/anti-cytokine spots within a 30 nl volume. In a set of proof-of-concept experiments, we demonstrate that ∼90% pure CD4 T-cells can be captured inside the device and that signals for three important T-cell secreted cytokines, tissue necrosis factor-alpha, interferon-gamma, and interleukin-2, may be enhanced by 2 to 3 folds through the use of reconfigurable microfluidics.

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Simultaneous detection of cell-secreted TNF-α and IFN-γ using micropatterned aptamer-modified electrodes

Cited by 137 publications

References 42 publications

Synthesis and electrocatalytic effect of Ag@Pt core–shell nanoparticles supported on reduced graphene oxide for sensitive and simple label-free electrochemical aptasensor

Synthesis and electrocatalytic effect of Ag@Pt core–shell nanoparticles supported on reduced graphene oxide for sensitive and simple label-free electrochemical aptasensor

Electrochemical Aptasensor Array for Multiple Detection of Human Osteopontin

Reconfigurable microfluidics combined with antibody microarrays for enhanced detection of T-cell secreted cytokines

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