Simultaneous detection of C-reactive protein and lipopolysaccharide based on a dual-channel electrochemical biosensor for rapid Gram-typing of bacterial sepsis

Lu, Tai-Cheng; Yang, Yuan-Jie; Zhong, Yu; Qiu, Qing-Zhen; Chen, Zhen-Hua; Chen, Yuan-Zhong; Lei, Yun; Liu, Ai-Lin

doi:10.1016/j.bios.2023.115772

Cited by 2 publications

(2 citation statements)

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“…In the work of Zinggeler et al [ 84 ], the UV illumination method was used for the immobilization of CRP antibodies through the process of crosslinking on an electrode substrate; the modified electrode achieved the amperometry technique using a wide range of CRP detection from 10 to 10,000 ng mL −1 . Lu et al [ 85 ] designed a CRP detection electrode using a dual-channel electrode assembled for the simultaneous lipopolysaccharide and CRP detection using a drop-casting approach on the gold electrode ( Figure 7 ). In short, a CRP binding antibody was cast on the Au surface and coated with a premixed CRP sample containing a non-specific interaction inhibition composition of biotin-labeled CRP antibody and bovine serum albumin (BSA).…”

Section: Electrochemical Detection Of Sepsis Biomarkersmentioning

confidence: 99%

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Challenges and Advances in Biomarker Detection for Rapid and Accurate Sepsis Diagnosis: An Electrochemical Approach

Kumar,

Banaś,

Krukiewicz

2024

Biosensors

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Sepsis is a life-threatening condition with high mortality rates due to delayed treatment of patients. The conventional methodology for blood diagnosis takes several hours, which suspends treatment, limits early drug administration, and affects the patient’s recovery. Thus, rapid, accurate, bedside (onsite), economical, and reliable sepsis biomarker reading of the clinical sample is an emergent need for patient lifesaving. Electrochemical label-free biosensors are specific and rapid devices that are able to perform analysis at the patient’s bedside; thus, they are considered an attractive methodology in a clinical setting. To reveal their full diagnostic potential, electrode architecture strategies of fabrication are highly desirable, particularly those able to preserve specific antibody–antigen attraction, restrict non-specific adsorption, and exhibit high sensitivity with a low detection limit for a target biomarker. The aim of this review is to provide state-of-the-art methodologies allowing the fabrication of ultrasensitive and highly selective electrochemical sensors for sepsis biomarkers. This review focuses on different methods of label-free biomarker sensors and discusses their advantages and disadvantages. Then, it highlights effective ways of avoiding false results and the role of molecular labels and functionalization. Recent literature on electrode materials and antibody grafting strategies is discussed, and the most efficient methodology for overcoming the non-specific attraction issues is listed. Finally, we discuss the existing electrode architecture for specific biomarker readers and promising tactics for achieving quick and low detection limits for sepsis biomarkers.

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Section: Electrochemical Detection Of Sepsis Biomarkersmentioning

confidence: 99%

“… Systematic electrode design for CRP and lipopolysaccharide concomitant detection using the TMB and H 2 O 2 electrocatalytic reduction method. Reprinted with permission from [ 85 ]. Copyright (2024) Elsevier.…”

Section: Figurementioning

confidence: 99%