Simultaneous detection of Anaplasma and Ehrlichia species in ruminants and detection of Ehrlichia ruminantium in Amblyomma variegatum ticks by reverse line blot hybridization

Bekker, Cornelis P. J.; Vos, Sander D. de; Taoufik, Amar; Sparagano, Olivier; Jongejan, Frans

doi:10.1016/s0378-1135(02)00179-7

Cited by 189 publications

(153 citation statements)

References 36 publications

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“…The RLB hybridization assay, which was developed for the simultaneous detection and identification of tick-borne parasites infecting cattle and small ruminants using 18S rRNA gene sequences (Bekker et al, 2002, Gubbels et al, 1999and Schnittger et al, 2003, has successfully been used to discover previously undescribed Theileria and Babesia species infecting African wildlife species (Nijhof et al, 2003, Nijhof et al, 2005and Oosthuizen et al, 2008. Subsequently 18S rRNA gene sequence analyses have been used to improve the descriptions of these species.…”

Section: Discussionmentioning

confidence: 99%

Identification of novel Babesia and Theileria species in South African giraffe (Giraffa camelopardalis, Linnaeus, 1758) and roan antelope (Hippotragus equinus, Desmarest 1804)

Oosthuizen

Allsopp

Troskie

et al. 2009

Veterinary Parasitology

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Section: Discussionmentioning

confidence: 99%

Identification of novel Babesia and Theileria species in South African giraffe (Giraffa camelopardalis, Linnaeus, 1758) and roan antelope (Hippotragus equinus, Desmarest 1804)

Oosthuizen

Allsopp

Troskie

et al. 2009

Veterinary Parasitology

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“…The Ehrlichia/Anaplasma PCR was performed with the forward primer Ehr-F (5 0 -GGA ATT CAG AGT TGG ATC MTG GYT CAG-3 0 ) and Ehr-R (5 0 -biotin-CGG GAT CCC GAG TTT GCC GGG ACT TYT TCT-3 0 ) amplifying a fragment of 460-520 bp from the V1 hypervariable region of the 16S SSU rRNA gene (Bekker et al, 2002;Nijhof et al, 2005). The conditions for the PCR included an initial step of 3 min at 42 8C, 10 min at 94 8C, 10 cycles of 94 8C (20 s)-67 8C (30 s)-728 C (30 s), with lowering of annealing step after every second cycle with 2 8C (touchdown PCR).…”

Section: Pcrmentioning

confidence: 99%

Molecular detection of tick-borne protozoal and ehrlichial infections in domestic dogs in South Africa

Matjila¹,

Leisewitz²,

Jongejan³

et al. 2008

Veterinary Parasitology

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A total of 1138 blood specimens were collected over a 6-year period (2000)(2001)(2002)(2003)(2004)(2005)(2006) from domestic dogs in South Africa. Specimens from domestic dogs were obtained from the Onderstepoort Veterinary Academic Hospital (OVAH) in Pretoria, the Society for the Prevention of Cruelty to Animals (Johannesburg, Durban, East London and Bloemfontein) and private practices from four provinces (Gauteng, Mpumalanga, KwaZulu-Natal and Western Cape). All specimens were screened for Babesia, Theileria, Hepatozoon and Ehrlichia/Anaplasma species using PCR and Reverse Line Blot (RLB) assays. On RLB, 560/1137 domestic dog-specimens were positive for one or more parasites. Of the positive domestic dog-specimens, 420 (75%) were infected with Babesia rossi; 82 (15%) dogs were infected with Theileria sp. (dog); 18 (3%) dogs were infected with Babesia vogeli; 14 (3%) specimens were infected with Ehrlichia canis. Mixed infections were also found: B. rossi and E. canis were detected in 12 (2%) specimens; B. vogeli and E. canis occurred in 7 (1%) specimens; Theileria sp. (dog) and E. canis in 3 (0.5%) specimens; B. rossi and B. vogeli in one specimen. B. rossi, B. vogeli and E. canis occurred simultaneously in one dog. There was also one incidental finding of a dog positive for Trypanosoma congolense. The results indicate that a wide range of tick-borne pathogens are circulating in the canine populations in South Africa. #

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“…Primers 16S8FE and B-GA1B-new were used to amplify a fragment of approximately 500 base pairs (bp) of the 16S rRNA gene spanning the V1 region of Anaplasma spp. and Ehrlichia spp., as described by Schouls et al (1999) and modified by Bekker et al (2002). Primers RLB-F2 and RLB-R2 were used to amplify a fragment of approximately 400 bp of the 18S SSU rRNA gene spanning the V4 region of Babesia spp.…”

Section: Dna Extractionmentioning

confidence: 99%

“…Polymerase chain reactions were performed in a Whatman BiometraH Tgradient basic thermocycler 96 (Gö ttingen, Germany) by using a touchdown PCR program (Bekker et al, 2002) with the following modifications: Uracil DNA glycosylase was not used and the annealing temperature was lowered by 1 C instead of 2 C.…”

Section: Dna Extractionmentioning

confidence: 99%

Ticks and Tick-Borne Pathogens From Wildlife in the Free State Province, South Africa

Tonetti

Berggoetz

Rühle

et al. 2009

Journal of Wildlife Diseases

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ABSTRACT:Eight ixodid tick species, associated with 59 free-ranging mammals belonging to 10 species, were collected at five different localities in the Free State Province, South Africa. Four of the study areas were nature reserves (Willem Pretorius, Sandveld, Tussen-die-Riviere, and Soetdoring), and one site was a private farm located in Senekal district. The collection was performed from March 2006 until June 2006. Ticks (n5569) and tissues from animals (n552) were analyzed by polymerase chain reaction, reverse line blot, and sequencing for various tickborne pathogens belonging to the genera Babesia, Theileria, Anaplasma, and Ehrlichia. Rhipicephalus (Boophilus) microplus, the known vector of Babesia bovis responsible for Asiatic redwater in South Africa, was found for the first time in the Free State Province. Rhipicephalus appendiculatus also was collected in areas in the Free State where it has not been previously described. Anaplasma marginale was detected for the first time in a gemsbok (Oryx gazella gazella). Gene sequences recovered in this study were 98-100% homologous with GenBank sequences for Anaplasma bovis, Theileria separata, and Theileria sp. Malelane sable antelope.

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Simultaneous detection of Anaplasma and Ehrlichia species in ruminants and detection of Ehrlichia ruminantium in Amblyomma variegatum ticks by reverse line blot hybridization

Cited by 189 publications

References 36 publications

Identification of novel Babesia and Theileria species in South African giraffe (Giraffa camelopardalis, Linnaeus, 1758) and roan antelope (Hippotragus equinus, Desmarest 1804)

Identification of novel Babesia and Theileria species in South African giraffe (Giraffa camelopardalis, Linnaeus, 1758) and roan antelope (Hippotragus equinus, Desmarest 1804)

Molecular detection of tick-borne protozoal and ehrlichial infections in domestic dogs in South Africa

Ticks and Tick-Borne Pathogens From Wildlife in the Free State Province, South Africa

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