Simultaneous detection of aflatoxin B1 and ochratoxin A in food samples by dual DNA tweezers nanomachine

Xiong, Zhengwei; Wang, Qiang; Xie, Yuejie; Li, Ning; Yun, Wen; Yang, Lizhu

doi:10.1016/j.foodchem.2020.128122

Cited by 65 publications

(28 citation statements)

References 36 publications

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“…Multiple mycotoxins can be produced from a single species of fungi, and it is not uncommon for foodstuffs to be simultaneously contaminated with multiple species of mycotoxin-producing fungi. Indeed, combinations of mycotoxins are detectable in contaminated food (Alshannaq and Yu, 2017;Jin et al, 2021;Xiong et al, 2021). Accordingly, it is feasible that the ingestion of contaminated foodstuff may result in exposure to multiple toxins which may exacerbate immunotoxicity.…”

Section: Combinatorial Exposure To Mycotoxinsmentioning

confidence: 99%

Fungal Toxins and Host Immune Responses

et al. 2021

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Fungi are ubiquitous organisms that thrive in diverse natural environments including soils, plants, animals, and the human body. In response to warmth, humidity, and moisture, certain fungi which grow on crops and harvested foodstuffs can produce mycotoxins; secondary metabolites which when ingested have a deleterious impact on health. Ongoing research indicates that some mycotoxins and, more recently, peptide toxins are also produced during active fungal infection in humans and experimental models. A combination of innate and adaptive immune recognition allows the host to eliminate invading pathogens from the body. However, imbalances in immune homeostasis often facilitate microbial infection. Despite the wide-ranging effects of fungal toxins on health, our understanding of toxin-mediated modulation of immune responses is incomplete. This review will explore the current understanding of fungal toxins and how they contribute to the modulation of host immunity.

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Section: Combinatorial Exposure To Mycotoxinsmentioning

confidence: 99%

Fungal Toxins and Host Immune Responses

et al. 2021

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“…Figure 2 A shows dual DNA tweezers nanomachine has been utilized for simultaneous detection of AFB1 and OTA [ 83 ]. The fluorophores were quenched by the quencher when DNA tweezers were closed.…”

Section: Rapid Detection Strategiesmentioning

confidence: 99%

“…(A) Dual DNA tweezers nanomachine for simultaneous detection of AFB1 and OTA; (B) a FRET-based platform using WS 2 nanosheet and dual-color gold nanoclusters (AuNCs) for simultaneous detection of AFB1 and ZEN; (C) a fluorescence aptasensor for FB1 and OTA detection by using TRF-NPs and MNPs; Diagrams A, B, and C are adapted from Ref. [ 83 ], Ref. [ 84 ], and Ref.…”

Section: Rapid Detection Strategiesmentioning

confidence: 99%

Recent advances in immunoassays and biosensors for mycotoxins detection in feedstuffs and foods

Yang

Wang

et al. 2021

J Animal Sci Biotechnol

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Mycotoxins are secondary metabolites produced by fungus. Many mycotoxin species are highly toxic and are frequently found in cereals and feedstuffs. So, powerful detection methods are vital and effective ways to prevent feed contamination. Traditional detection methods can no longer meet the needs of massive, real-time, simple, and fast mycotoxin monitoring. Rapid detection methods based on advanced material and sensor technology are the future trend. In this review, we highlight recent progress of mycotoxin rapid detection strategies in feedstuffs and foods, especially for simultaneous multiplex mycotoxin determination. Immunoassays, biosensors, and the prominent roles of nanomaterials are introduced. The principles of different types of recognition and signal transduction are explained, and the merits and pitfalls of these methods are compared. Furthermore, limitations and challenges of existing rapid sensing strategies and perspectives of future research are discussed.

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“…Aflatoxin B1 has been listed as group 1 carcinogens by the international agency. [11] In addition, aflatoxin B1 possesses high chemical stability, making it difficult to be damaged by cooking or other machining process. [12] It is important to explore highly sensitive and reliable method for detecting aflatoxin B1 to ensure food safety.…”

Section: Ag@au-fe 3 O 4 -Mrs Immunosensor For Detection Of Aflatoxin B1mentioning

confidence: 99%

Magnetic Relaxation Switching Immunoassay Based on Hydrogen Peroxide‐Mediated Assembly of Ag@Au–Fe₃O₄ Nanoprobe for Detection of Aflatoxin B1

Dong

Wen

She

et al. 2021

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Magnetic relaxation switching (MRS) sensors have shown great potential in food safety monitoring due to their high signal‐to‐noise ratio and simplicity, but they often suffer from insufficient sensitivity and stability due to the lack of excellent magnetic nanoprobes. Herein, dumbbell‐like Au–Fe3O4 nanoparticles are designed as magnetic nanoprobes for developing an aflatoxin B1‐MRS immunosensor. The Fe3O4 portion in the Au–Fe3O4 nanoparticles functions as the magnetic probe to provide transverse relaxation signals, while the Au segments serve as a bridge to grow Ag shell and assemble the Au–Fe3O4 nanoparticles, thus modulating transverse relaxation time of surrounding water molecular. The formation of Ag@Au–Fe3O4 is triggered by hydrogen peroxide. After degraded by horseradish peroxidase, hydrogen peroxide reduces Ag+ to Ag nanoparticles which assemble dispersed Au–Fe3O4 to aggregated Ag@Au–Fe3O4, thus dramatically improving the sensitivity of traditional MRS sensor. Combined with competitive immunoreaction, this Ag@Au–Fe3O4–MRS immunosensor can detect aflatoxin B1 with a high sensitivity (3.81 pg mL−1), which improved about 21 folds and 9 folds than those of enzyme‐linked immunosorbent assay and high‐performance liquid chromatography (HPLC), respectively. The good consistency with HPLC in real samples detection indicates the good accuracy of this immunosensor. This Ag@Au–Fe3O4–MRS immunosensor offers an attractive tool for detection of harmful substances.

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Simultaneous detection of aflatoxin B1 and ochratoxin A in food samples by dual DNA tweezers nanomachine

Cited by 65 publications

References 36 publications

Fungal Toxins and Host Immune Responses

Fungal Toxins and Host Immune Responses

Recent advances in immunoassays and biosensors for mycotoxins detection in feedstuffs and foods

Magnetic Relaxation Switching Immunoassay Based on Hydrogen Peroxide‐Mediated Assembly of Ag@Au–Fe₃O₄ Nanoprobe for Detection of Aflatoxin B1

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Simultaneous detection of aflatoxin B1 and ochratoxin A in food samples by dual DNA tweezers nanomachine

Cited by 65 publications

References 36 publications

Fungal Toxins and Host Immune Responses

Fungal Toxins and Host Immune Responses

Recent advances in immunoassays and biosensors for mycotoxins detection in feedstuffs and foods

Magnetic Relaxation Switching Immunoassay Based on Hydrogen Peroxide‐Mediated Assembly of Ag@Au–Fe3O4 Nanoprobe for Detection of Aflatoxin B1

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Magnetic Relaxation Switching Immunoassay Based on Hydrogen Peroxide‐Mediated Assembly of Ag@Au–Fe₃O₄ Nanoprobe for Detection of Aflatoxin B1