Simultaneous detection and differentiation of Newcastle disease and avian influenza viruses using oligonucleotide microarrays

Wang, Lih-Chiann; Pan, Chu-Hsiang; Severinghaus, Lucia Liu; Liu, Lu-Yuan; Chen, Chi-Tsong; Pu, Chang-En; Huang, De-An; Lir, Jihn-Tsair; Chin, Shih-Chien; Cheng, Ming‐Chu; Lee, Shu-Hwae; Wang, Ching-Ho

doi:10.1016/j.vetmic.2007.08.019

Cited by 40 publications

(17 citation statements)

References 32 publications

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“…The probe, target, recovery, and noncomplementary DNA sequences designed for detecting the avian influenza virus (AIV) 29,30 are shown in Table 2. The I D -V G curve of the DNA probe-modified pSNWFET obtained in 10 mM Na-PB (pH 7.0) was used as the baseline (black).…”

Section: Representative Resultsmentioning

confidence: 99%

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Lin

Feng

et al. 2016

JoVE

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Surveillance using biomarkers is critical for the early detection, rapid intervention, and reduction in the incidence of diseases. In this study, we describe the preparation of polycrystalline silicon nanowire field-effect transistors (pSNWFETs) that serve as biosensing devices for biomarker detection. A protocol for chemical and biomolecular sensing by using pSNWFETs is presented. The pSNWFET device was demonstrated to be a promising transducer for real-time, label-free, and ultra-high-sensitivity biosensing applications. The source/drain channel conductivity of a pSNWFET is sensitive to changes in the environment around its silicon nanowire (SNW) surface. Thus, by immobilizing probes on the SNW surface, the pSNWFET can be used to detect various biotargets ranging from small molecules (dopamine) to macromolecules (DNA and proteins). Immobilizing a bioprobe on the SNW surface, which is a multistep procedure, is vital for determining the specificity of the biosensor. It is essential that every step of the immobilization procedure is correctly performed. We verified surface modifications by directly observing the shift in the electric properties of the pSNWFET following each modification step. Additionally, X-ray photoelectron spectroscopy was used to examine the surface composition following each modification. Finally, we demonstrated DNA sensing on the pSNWFET. This protocol provides step-by-step procedures for verifying bioprobe immobilization and subsequent DNA biosensing application. Video LinkThe video component of this article can be found at

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Section: Representative Resultsmentioning

confidence: 99%

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Lin

Feng

et al. 2016

JoVE

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“…Accordingly, all 43 influenza A virus isolates of all 16 HA subtypes tested were readily detected, and the HA subtypes were accurately determined. Microarrays for the detection and the characterization of influenza A viruses and other pathogens have been described previously (9,10,18,20,21,22,23,24,25,27,29,32,33,38,39). The assay reported here combines a one-step RT-PCR by use of a commercially available kit with a low-density microarray assay for characterization of the amplicons without further purification.…”

Section: Discussionmentioning

confidence: 99%

“…Microarray assays, which are capable of detecting multiple targets in parallel, open new possibilities, including the in-depth analysis of virus isolates within a short turnaround time. Recently, microarray assays have been described as valuable methods for the detection, differential diagnosis, and subtyping (of the H1, H2, H3, H5, N1, and N2 subtypes) of influenza A viruses with relevance to human disease (9,10,18,20,21,22,27,28,38,39), as well as for HA and NA subtyping (23,24,32,33).…”

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confidence: 99%

Design and Validation of a Microarray for Detection, Hemagglutinin Subtyping, and Pathotyping of Avian Influenza Viruses

et al. 2009

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“…Visual comparison is not limited to such data verification purposes, however. Wang et al [12] describes a method for quick bird virus detection based on microarray data. The method involves comparing microarray results with the "naked eye", and claims to be a rapid way of distinguishing between a non-infected sample and Newcastle disease or avian influenza.…”

Section: Discussionmentioning

confidence: 99%

Visual Comparison of Multiple Gene Expression Datasets in a Genomic Context

Borowski

Soh

Sensen

2008

Journal of Integrative Bioinformatics

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SummaryThe need for novel methods of visualizing microarray data is growing. New perspectives are beneficial to finding patterns in expression data. The Bluejay genome browser provides an integrative way of visualizing gene expression datasets in a genomic context. We have now developed the functionality to display multiple microarray datasets simultaneously in Bluejay, in order to provide researchers with a comprehensive view of their datasets linked to a graphical representation of gene function. This will enable biologists to obtain valuable insights on expression patterns, by allowing them to analyze the expression values in relation to the gene locations as well as to compare expression profiles of related genomes or of different experiments for the same genome.

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Simultaneous detection and differentiation of Newcastle disease and avian influenza viruses using oligonucleotide microarrays

Cited by 40 publications

References 32 publications

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Preparation of Silicon Nanowire Field-effect Transistor for Chemical and Biosensing Applications

Design and Validation of a Microarray for Detection, Hemagglutinin Subtyping, and Pathotyping of Avian Influenza Viruses

Visual Comparison of Multiple Gene Expression Datasets in a Genomic Context

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