Simultaneous densitometric determination of anthelmintic drug albendazole and its metabolite albendazole sulfoxide by HPTLC in human plasma and pharmaceutical formulations

Abstract

A new, simple, accurate and precise high-performance thin-layer chromatographic method has been developed and validated for simultaneous determination of an anthelmintic drug, albendazole, and its active metabolite albendazole, sulfoxide. Planar chromatographic separation was performed on aluminum-backed layer of silica gel 60G F using a mixture of toluene-acetonitrile-glacial acetic acid (7.0:2.9:0.1, v/v/v) as the mobile phase. For quantitation, the separated spots were scanned densitometrically at 225 nm. The retention factors (R ) obtained under the established conditions were 0.76 ± 0.01 and 0.50 ± 0.01 and the regression plots were linear (r ≥ 0.9997) in the concentration ranges 50-350 and 100-700 ng/band for albendazole and albendazole sulfoxide, respectively. The method was validated for linearity, specificity, accuracy (recovery) and precision, repeatability, stability and robustness. The limit of detection and limit of quantitation found were 9.84 and 29.81 ng/band for albendazole and 21.60 and 65.45 ng/band for albendazole sulfoxide, respectively. For plasma samples, solid-phase extraction of analytes yielded mean extraction recoveries of 87.59 and 87.13% for albendazole and albendazole sulfoxide, respectively. The method was successfully applied for the analysis of albendazole in pharmaceutical formulations with accuracy ≥99.32%.

Facile complexation reactions for the selective spectrofluorimetric determination of albendazole in oral dosage forms and spiked human plasma

“… 2 Due to the great clinical importance of ABZ, several analytical methods have been reported for its determination either in pharmaceutical dosage forms or in biological fluids. The reported analytical methods include: spectrophotometry, 3–9 spectrofluorimetry, 10–13 high-performance liquid chromatography (HPLC) 14–17 HPTLC, 18 thermogravimetry 19 and electrochemical methods. 20,21 …”

LC–MS/MS method for simultaneous determination of diethylcarbamazine, albendazole and albendazole metabolites in human plasma: Application to a clinical pharmacokinetic study

“…Albendazole is only detectable for a short time in plasma due to its rapid conversion to its major active metabolites such as ABZ-sulfoxide (ABZ-OX) and ABZ-sulfone (ABZ-ON) and subsequently low drug concentrations [ 6 ]. Various bioanalytical methods are available for analysis of ABZ and its metabolites in different biological matrices [ [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] ]. Wojnicz et al reported a LC–MS/MS method for the quantitation of ABZ and ABZ-OX in 200 μL plasma with a LLOQ of 5 and 10 ng/mL)[ 10 ].…”

“…Rathod et al developed a LC–MS/MS method for the quantitation of ABZ and ABZ-OX in 100 μL plasma with a LLOQ of 0.3and 3 ng/mL respectively [ 13 ]. Pandya et al has a developed a HPTLC method for ABZ and ABZ-OX, has a LLOQ of and 50 and100 ng/mL[ 14 ].…”

Novel albendazole-glucan particles for enhancing intestinal absorption and improving hepatic targeting

“…As the preferred anti-hydatid drug, ABZ has a very fast metabolic rate in plasma ( 35 ). After oral administration, ABZ is rapidly transformed into ABZ sulphoxide (ABZSO) and ABZSO 2 (of which ABZSO is the main active substance) and finally into albendazole-2-aminosulfone (ABZSO 2 -NH 2 ) under the action of various enzyme systems in the liver ( 36 ).…”