Simultaneous Coinfection of Macaques with Zika and Dengue Viruses Does not Enhance Acute Plasma Viremia but Leads to Activation of Monocyte Subsets and Biphasic Release of Pro-inflammatory Cytokines

Valiant, William G.; Mattapallil, Mary J.; Higgs, Stephen; Huang, Yan-Jang S.; Vanlandingham, Dana L.; Lewis, Mark G.; Mattapallil, Joseph J.

doi:10.1038/s41598-019-44323-y

Cited by 15 publications

(8 citation statements)

References 87 publications

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“…There are scenarios where an infection with a given pathogen predisposes the individual host to secondary pathogens through immunosuppression [ 367 , 368 ]. In addition, pathogens of the same or similar geographical distribution, ecological tolerance, or sharing the same vectors are likely to co-infect the same individual host [ 369 , 370 , 371 ]. Antagonistic relationships between different pathogens within an individual host have also been reported.…”

Section: Clinical Presentation Epidemiology Infection Sequalae mentioning

confidence: 99%

West Nile Virus: An Update on Pathobiology, Epidemiology, Diagnostics, Control and “One Health” Implications

et al. 2020

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West Nile virus (WNV) is an important zoonotic flavivirus responsible for mild fever to severe, lethal neuroinvasive disease in humans, horses, birds, and other wildlife species. Since its discovery, WNV has caused multiple human and animal disease outbreaks in all continents, except Antarctica. Infections are associated with economic losses, mainly due to the cost of treatment of infected patients, control programmes, and loss of animals and animal products. The pathogenesis of WNV has been extensively investigated in natural hosts as well as in several animal models, including rodents, lagomorphs, birds, and reptiles. However, most of the proposed pathogenesis hypotheses remain contentious, and much remains to be elucidated. At the same time, the unavailability of specific antiviral treatment or effective and safe vaccines contribute to the perpetuation of the disease and regular occurrence of outbreaks in both endemic and non-endemic areas. Moreover, globalisation and climate change are also important drivers of the emergence and re-emergence of the virus and disease. Here, we give an update of the pathobiology, epidemiology, diagnostics, control, and “One Health” implications of WNV infection and disease.

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Section: Clinical Presentation Epidemiology Infection Sequalae mentioning

confidence: 99%

West Nile Virus: An Update on Pathobiology, Epidemiology, Diagnostics, Control and “One Health” Implications

et al. 2020

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“…Additionally, the disease can be transmitted through non-veterinary mechanisms such as blood transfusions and sexual contact as well as from mother to fetus, causing microcephaly [5]. Moreover, co-infection of Zika and dengue viruses is dangerous and causes acute tissue damage and transient anemia [6]. Therefore, if a visitor to an area where Zika virus is prevalent shows suspicious symptoms, a means to determine whether the individual is infected is required.…”

Section: Introductionmentioning

confidence: 99%

Selection of DNA aptamer and its application as an electrical biosensor for Zika virus detection in human serum

et al. 2022

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Zika virus is a highly infectious virus that is part of the flavivirus group. Precise diagnosis of the Zika virus is significant issue for controlling a global pandemic after the COVID-19 era. For the first time, we describe a zika virus aptamer-based electrical biosensor for detecting Zika virus in human serum. The electrical biosensor composed of a Zika virus aptamer/MXene nanoparticle heterolayer on Au micro-gap electrode (AuMGE)/print circuit board (PCB) system. The Zika virus aptamer was designed to bind the envelope protein of the Zika virus by systematic evolution of ligands by exponential enrichment (SELEX) technique. The binding affinity of the aptamer was determined by fluorescence. For improving the sensor signal sensitivity, Ti3C2Tx MXene was introduced to surface of Au micro-gap electrode (AuMGE). The immobilization process was confirmed by atomic force microscopy (AFM). The prepared aptamer/MXene immobilized on AuMGE can detect the Zika virus through capacitance change according to the target concentration. The capacitance signal from the biosensor increased linearly according to increment of envelope proteins in the human serum. The limit of detection was determined to 38.14 pM, and target proteins could be detected from 100 pM to 10 μM. Thus, the developed electrical aptabiosensor can be a useful tool for Zika virus detection.

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“…Plasma cytokine levels were determined at the Immunology Unit of the Duke Human Vaccine Institute using the Cytokine Monkey Magnetic 29-Plex Panel for Luminex ™ Platform (Thermofisher Scientific, Waltham, MA). This kit can simultaneously quantify 29 cytokines namely, FGF-basic, IL-1β, G-CSF, IL-10, IL-6, IL-12, RANTES, Eotaxin, IL-17, MIP-1α, GM-CSF, MIP-1β, MCP-1, IL-15, EGF, IL-5, HGF, VEGF, IFNγ, MDC, I-TAC, MIF, IL-1RA, TNFα, IL-2, IP-10, MIG, IL-4 and IL-8 in rhesus macaques[53, 54]. Plasma samples were setup in triplicates and the plates were analyzed using Luminex xMAP technology on a Bio-plex 200 system (Biorad).…”

Section: Methodsmentioning

confidence: 99%

Gender differences in innate responses and gene expression profiles in memory CD4 T cells are apparent very early during acute simian immunodeficiency virus infection

George¹,

Johnson²,

Mattapallil³

et al. 2019

PLoS ONE

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Gender differences in Human immunodeficiency virus (HIV) disease progression and comorbidities have been extensively reported. Using the simian immunodeficiency virus (SIV) infected rhesus macaque model, we show that these differences are apparent very early during the course of infection. Though there were no major changes in the proportions of CD4 T cells or its subsets, central memory CD4 T cells from female macaques were found to differentially regulate a significantly larger number of genes at day 4 post-infection (PI) as compared to males. Pathway analysis revealed divergence of both canonical and biological pathways that persisted at day 10 PI. Changes in gene expression profiles were accompanied by a significant increase in plasma levels of pro-inflammatory mediators such as MCP-1/CCL2, I-TAC/CXCL11, and MIF. Though plasma levels of IFNα did not differ between male and female macaques, the expression levels of IFNα subtype-14, 16, IFNβ, and IFNω were significantly upregulated in the lymph nodes of female macaques at day 10 PI as compared to male macaques. Our results suggest that the pathogenic sequelae seen during chronic infection may be shaped by gender differences in immune responses induced very early during the course of HIV infection.

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Simultaneous Coinfection of Macaques with Zika and Dengue Viruses Does not Enhance Acute Plasma Viremia but Leads to Activation of Monocyte Subsets and Biphasic Release of Pro-inflammatory Cytokines

Cited by 15 publications

References 87 publications

West Nile Virus: An Update on Pathobiology, Epidemiology, Diagnostics, Control and “One Health” Implications

West Nile Virus: An Update on Pathobiology, Epidemiology, Diagnostics, Control and “One Health” Implications

Selection of DNA aptamer and its application as an electrical biosensor for Zika virus detection in human serum

Gender differences in innate responses and gene expression profiles in memory CD4 T cells are apparent very early during acute simian immunodeficiency virus infection

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