Simultaneous Chiral Analysis of Methamphetamine and its Metabolites by Capillary Electrophoresis/Mass Spectrometry with Direct Injection of Urine

Iio, Reiko; Chinaka, Satoshi; Takayama, Nariaki; Hayakawa, Kazuichi

doi:10.1248/jhs.51.693

Cited by 28 publications

(27 citation statements)

References 26 publications

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“…Furthermore, the total analysis times could be reduced since off-line sample pretreatment steps such as LLE and SPE, which are usually necessary for urine analysis, were not required. Accordingly, this will be useful when the testing of a large quantity of samples is required within a predetermined period of time, as in doping tests for the Olympic games [52,53], or when the inspection time is limited by the law [54].…”

Section: Application To Urine Samplesmentioning

confidence: 99%

Direct chiral analysis of primary amine drugs in human urine by single drop microextraction in‐line coupled to CE

et al. 2009

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Three-phase single drop microextraction (SDME) was in-line coupled to chiral CE of weakly basic amine compounds including amphetamine. SDME was used for the matrix isolation and sample preconcentration in order to directly analyze urine samples with the minimal pretreatment of adding NaOH. A small drop of an acidic aqueous acceptor phase covered with a thin layer of octanol was formed at the tip of a capillary by simple manipulation of the liquid handling functions of a commercial CE instrument. While the saline matrix of the urine sample was blocked by the octanol layer, the basic analytes in a basic aqueous donor phase were concentrated into the acidic acceptor drop through the octanol layer by the driving force of the pH difference between the two aqueous phases. The enantiomers of the enriched amines were resolved by using (+)-(18-crown-6)-tetracarboxylic acid as a chiral selector for the subsequent CE separation. From 10 min SDME with the agitation of the donor phase by a small stirrer retrofit to the CE instrument, enrichment factors were about a 1000-fold, yielding the LOD of 0.5 ng/mL for amphetamine. This low LOD value as well as the convenience of in-line coupled SDME make the proposed scheme well suited for the demanding chiral analysis of amphetamine-type stimulants.

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Section: Application To Urine Samplesmentioning

confidence: 99%

Direct chiral analysis of primary amine drugs in human urine by single drop microextraction in‐line coupled to CE

et al. 2009

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“…On the other hand, analysis times are quite short for this kind of analysis: nine enantiomeric pairs are analysed in 9 min. The same research group had already published some other papers on the same topic in the past, dealing with a lower number of compounds and using the same or very similar dualcyclodextrin selector systems [29][30][31][32][33][34], sometimes using MS detection to apply the method to urine.…”

Section: Amphetaminesmentioning

confidence: 99%

Chiral analysis of amphetamines, methadone and metabolites in biological samples by electrodriven methods

Mandrioli¹,

Mercolini²,

Raggi³

2011

Electrophoresis

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Amphetamines and methadone are synthetic chiral drugs with a high potential for abuse. As such, several analytical methods have been developed for their enantioseparation and analysis in biological tissues, and some of these are based on electrodriven techniques. In this review, the most important and recent of these latter methods are reviewed and their main advantages and disadvantages are discussed. Particular attention is paid to the suitability of each method for the application to the biological matrix of interest: while all methods have been successfully applied for one or more biological tissues, to reach this goal they must overcome the sensitivity problem that is common to almost all capillary electrophoretic techniques. Most methods use one or more cyclodextrin derivatives as the chiral selector, thus the separation mechanism is not particularly complicated or unusual.

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“…They mention as the main drawback of UVdetection its relatively low sensitivity (10 -5 -10 -6 M). Comparatively, the on-line coupling of CE with MS is an attractive technique for the analysis of erythropoietin, because MS detection has the advantage over both UV-Vis and LIF detection [45]. Nevertheless, detection of rhEPO by the on-line CE-ESI-MS technology is still subject to various difficulties, including the concentration of CE buffer, the type, composition and flow-rate of sheath liquid, and the temperature and flow rate of drying gas.…”

Section: Proteinsmentioning

confidence: 99%

Capillary Electrophoresis Coupled To Mass Spectrometry for Forensic Analysis

Huck¹,

Huck-Pezzei²,

Bakry³

et al. 2007

TOCENGJ

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Abstract:In this review the most important techniques, which are developed to hyphenate capillary electrophoresis to mass spectrometry (CE-MS), suitable for forensic analysis, are summarized. Analytes of interest are divided into four main parts, namely, compounds with amine containing side chains, compounds with N-containing saturated ring structures, other heterocycles and peptides. Sample pre-treatments and direct injection modes used in CE-MS for forensic analysis are briefly discussed from critical point of view. Special emphasis is placed to point out the advantages of mass spectrometric detection compared to UV-and laser-induced fluorescence (LIF) detections.

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Simultaneous Chiral Analysis of Methamphetamine and its Metabolites by Capillary Electrophoresis/Mass Spectrometry with Direct Injection of Urine

Cited by 28 publications

References 26 publications

Direct chiral analysis of primary amine drugs in human urine by single drop microextraction in‐line coupled to CE

Direct chiral analysis of primary amine drugs in human urine by single drop microextraction in‐line coupled to CE

Chiral analysis of amphetamines, methadone and metabolites in biological samples by electrodriven methods

Capillary Electrophoresis Coupled To Mass Spectrometry for Forensic Analysis

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