Simultaneous chemical induction of MTV and MLV in vitro

Links, J.; Calafat, Jero; Buijs, F.; Tol, Olga

doi:10.1016/0014-2964(77)90119-0

Cited by 7 publications

(3 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although MMTV is a causative agent of carcinomas of the mammary gland, viral expression has also been demonstrated in other organs, including the kidney of MMTV-infected mice (3,40). Moreover, BALB/c primary kidney cells have been shown to support MMTV replication in tissue culture (35). These observations suggest that the kidney may constitute a potential target for pathological alterations by MMTV.…”

Section: Discussionmentioning

confidence: 91%

Exogenous mouse mammary tumor virus proviral DNA isolated from a kidney adenocarcinoma cell line contains alterations in the U3 region of the long terminal repeat

Wellinger¹,

Garcia²,

Vessaz³

et al. 1986

J Virol

View full text Add to dashboard Cite

Mouse mammary tumor virus (MMTV) is a B-type retrovirus which induces predominantly mammary carcinomas after a relatively long latency period. To date, very little is known about the reasons for the strict tissue specificity of MMTV. The BALB/cf/Cd strain of mice, which was infected with milk-borne MMTV(C3H), shows a high incidence of kidney adenocarcinomas, and our data suggest that MMTV might be involved in the formation of these tumors. Newly integrated exogenous MMTV proviruses were found in the genome of transplanted tumor cells as well as in the DNA of a cell line derived from one tumor, but not in normal cells of BALB/cf/Cd mice. The MMTV DNA in these tumor cells was transcribed and viral RNA synthesis was strongly stimulated by glucocorticoid hormones. Viral structural polypeptides, comparable in size and antigenicity to MMTV polypeptides of infected mammary tumor cells were synthesized and processed normally in the cell line and were organized correctly into intracytoplasmic particles. Heteroduplex analysis of the molecularly cloned MMTV proviral DNAs of kidney and mammary tumor origin revealed a high degree of homology in the gag, pol, and env genes. A striking difference, however, was observed in the U3 region of the two LTRs that might relate to the different tissue specificity of the two viruses.

show abstract

Section: Discussionmentioning

confidence: 91%

Exogenous mouse mammary tumor virus proviral DNA isolated from a kidney adenocarcinoma cell line contains alterations in the U3 region of the long terminal repeat

Wellinger¹,

Garcia²,

Vessaz³

et al. 1986

J Virol

View full text Add to dashboard Cite

show abstract

“…Normal mice not containing Mtv-I and Mtv-2 can form mammary tumors, which are most likely due to other endogenous MMTV, after 1 yr of age (57). Furthermore, treatment of laboratory mouse strains or mouse cell lines with irradiation and/or carcinogens can lead to formation of infectious MMTV particles (64)(65)(66)(67)(68). Although further experiments are required to definitively settle this question, it seems likely that the virus was transmitted to BALB/c IC mice from outbred Swiss mice.…”

Section: Discussionmentioning

confidence: 99%

An exogenous mouse mammary tumor virus with properties of Mls-1a (Mtv-7).

Held

Shakhov

Waanders

et al. 1992

The Journal of Experimental Medicine

132

144

View full text Add to dashboard Cite

The classical minor lymphocyte stimulating (Mls) antigens, which induce a strong primary T cell response in vitro, are closely linked to endogenous copies of mouse mammary tumor viruses (MMTV). Expression of Mls genes leads to clonal deletion of T cell subsets expressing specific T cell receptor (TCR) V beta chains. We describe the isolation and characterization of a new exogenous (infectious) MMTV with biological properties similar to the Mls antigen Mls-1a. In vivo administration of either Mls-1a-expressing B cells or the infectious MMTV (SW) led to an increase of T cells expressing V beta 6 followed by their deletion. Surprisingly, different kinetics of deletion were observed with the exogenous virus depending upon the route of infection. Infection through the mucosa led to a slow deletion of V beta 6+ T cells, whereas deletion was rapid after subcutaneous infection. Sequence analysis of the open reading frames in the 3' long terminal repeat of both this exogenous MMTV (SW) and of Mtv-7 (which is closely linked to Mls-1a) revealed striking similarities, particularly in the COOH terminus, which has been implicated in TCR V beta recognition. The identification of an infectious MMTV with the properties of a strong Mls antigen provides a new, powerful tool to study immunity and tolerance in vivo.

show abstract

“…Previous reports agree that exposure to chemical carcinogens and/or irradiation can activate MMTV expression (11,14,26,29). However, whether the activation phenomenon is causally related to the neoplastic process or is secondary to other changes in cell regulation during tumorigenesis has not been determined.…”

mentioning

confidence: 98%

Detection of Mouse Mammary Tumor Virus RNA in BALB/c Tumor Cell Lines of Nonviral Etiologies

Dudley

Butel

Socher

et al. 1978

J Virol

View full text Add to dashboard Cite

A complementary DNA (cDNA) probe to mouse mammary tumor virus (MMTV) RNA was synthesized using calf thymus DNA oligonucleotides as a random primer. This probe was then used to study the expression of MMTV RNA in cell lines from BALB/c tumors induced in vivo either spontaneously or in response to viral, chemical, or hormonal stimuli. The cDNA had a length of approximately 400 to 500 nucleotides and specifically hybridized to MMTV RNA and BALB/c lactating mammary gland RNA, but not to Moloney leukemia virus RNA. Calf thymus DNA-primed cDNA could protect 50% of iodinated MMTV RNA from Si nuclease digestion at cDNA-RNA ratios of 1:1 and 90% of labeled viral RNA at ratios of 10:1. Thermal denaturation of MMTV RNA-cDNA hybrids yielded a Tm of 88.5°C, indicative of a well-base-paired duplex. Screening of mouse matnmary tumor cells for MMTV sequences revealed that three out of five lines of BALB/c origin had undetectable levels of viral RNA (

show abstract

Simultaneous chemical induction of MTV and MLV in vitro

Cited by 7 publications

References 23 publications

Exogenous mouse mammary tumor virus proviral DNA isolated from a kidney adenocarcinoma cell line contains alterations in the U3 region of the long terminal repeat

Exogenous mouse mammary tumor virus proviral DNA isolated from a kidney adenocarcinoma cell line contains alterations in the U3 region of the long terminal repeat

An exogenous mouse mammary tumor virus with properties of Mls-1a (Mtv-7).

Detection of Mouse Mammary Tumor Virus RNA in BALB/c Tumor Cell Lines of Nonviral Etiologies

Contact Info

Product

Resources

About