Simultaneous butyrate oxidation by Syntrophomonas wolfei and catalytic olefin reduction in absence of interspecies hydrogen transfer

Kaspar, Heinrich F.; Holland, Arnold J.; Mountfort, Douglas O.

doi:10.1007/bf00406129

Cited by 15 publications

(8 citation statements)

References 18 publications

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“…When the coculture was incubated with 1.5 mg/mL bromoethanesulfonic acid (a potent methanogen inhibitor; see Kaspar et al, 1987), no methane was detected and there was no microscopic evidence of the methanogen. Also, incubation of T. maritima with M. jannaschii cell extract or concentrated supernatant (from M. jannaschii cultures) did not result in any significant differences from the cultures of T. maritima used as controls.…”

Section: Coculture Characteristicsmentioning

confidence: 99%

Hydrogen transfer between methanogens and fermentative heterotrophs in hyperthermophilic cocultures

Muralidharan¹,

Rinker²,

Hirsh³

et al. 1997

Biotechnol. Bioeng.

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Abstract:Interactions involving hydrogen transfer were studied in a coculture of two hyperthermophilic microorganisms: Thermotoga maritima, an anaerobic heterotroph, and Methanococcus jannaschii, a hydrogenotrophic methanogen. Cell densities of T. maritima increased 10-fold when cocultured with M. jannaschii at 85°C, and the methanogen was able to grow in the absence of externally supplied H 2 and CO 2 . The coculture could not be established if the two organisms were physically separated by a dialysis membrane, suggesting the importance of spatial proximity. The significance of spatial proximity was also supported by cell cytometry, where the methanogen was only found in cell sorts at or above 4.5 µm in samples of the coculture in exponential phase. An unstructured mathematical model was used to compare the influence of hydrogen transport and metabolic properties on mesophilic and hyperthermophilic cocultures. Calculations suggest the increases in methanogenesis rates with temperature result from greater interactions between the methanogenic and fermentative organisms, as evidenced by the sharp decline in H 2 concentration in the proximity of a hyperthermophilic methanogen. The experimental and modeling results presented here illustrate the need to consider the interactions within hyperthermophilic consortia when choosing isolation strategies and evaluating biotransformations at elevated temperatures.

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Section: Coculture Characteristicsmentioning

confidence: 99%

Hydrogen transfer between methanogens and fermentative heterotrophs in hyperthermophilic cocultures

Muralidharan¹,

Rinker²,

Hirsh³

et al. 1997

Biotechnol. Bioeng.

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“…This reaction can be carried out by hydrogen-oxidizing bacteria in syntrophic coculture [10,[26][27][28][29][30]. Recently, effective chemical mechanisms have also been demonstrated [31]. The necessity for H 2 removal can easily be explained by the fact that the H2-producing reactions are endergonic or only poorly exergonic at standard conditions and pH 7.0, i.e., at a hydrogen partial pressure of approximately 100 kPa.…”

Section: Desulfovibrio Vulgaris and Pelobacter Acetylenicusmentioning

confidence: 99%

Thermodynamics of hydrogen metabolism in methanogenic cocultures degrading ethanol or lactate

Seitz¹,

Schink²,

Conrad³

1988

FEMS Microbiology Letters

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Pure cultures of Desulfovibrio vulgaris or Pelobacter acetylenicus do not grow with lactate or ethanol, respectively, under obligately proton‐ reducing conditions. However, a small part of these substrates was oxidized and molecular hydrogen was produced up to 4.2 and 3.2 kPa, respectively. During growth in syntrophic methanogenic cocultures with Methanospirillum hungatei as partner, maximum hydrogen partial pressures were significantly lower (0.7 to 2.5 kPa) than in the corresponding pure cultures. Calculation of Gibbs free energies for the prevailing culture conditions showed that H2 partial pressures were kept in a range at which both, H2‐producing and H2‐consuming reactions, were thermodynamically permissive in pure as well as in syntrophic mixed cultures.

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“…Since this achievement there have been only sporadic examples of systems that directly combine reagents from synthetic organic chemistry with metabolism. Mountfort and coworkers reported the hydrogenation of ethylene using hydrogen gas produced by the syntroph Syntrophomonas wolfei and super-stoichiometric amounts of a heterogeneous palladium catalyst, a transformation that disrupted the metabolic interaction between S. wolfei and the methanogen Methanospirillum hungatei [47]. Other researchers have used transition metal catalysts to hydrogenate membrane lipids in the cyanobacterium Synechocystis sp.…”

Section: Integrating Organic Chemistry With Cellular Metabolismmentioning

confidence: 99%

Opportunities for merging chemical and biological synthesis

Wallace

Balskus

2014

Current Opinion in Biotechnology

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Organic chemists and metabolic engineers use largely orthogonal technologies to access small molecules like pharmaceuticals and commodity chemicals. As the use of biological catalysts and engineered organisms for chemical production grows, it is becoming increasingly evident that future efforts for chemical manufacture will benefit from the integration and unified expansion of these two fields. This review will discuss approaches that combine chemical and biological synthesis for small molecule production. We highlight recent advances in combining enzymatic and non-enzymatic catalysis in vitro, discuss the application of design principles from organic chemistry for engineering non-biological reactivity into enzymes, and describe the development of biocompatible chemistry that can be interfaced with microbial metabolism.

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Simultaneous butyrate oxidation by Syntrophomonas wolfei and catalytic olefin reduction in absence of interspecies hydrogen transfer

Cited by 15 publications

References 18 publications

Hydrogen transfer between methanogens and fermentative heterotrophs in hyperthermophilic cocultures

Hydrogen transfer between methanogens and fermentative heterotrophs in hyperthermophilic cocultures

Thermodynamics of hydrogen metabolism in methanogenic cocultures degrading ethanol or lactate

Opportunities for merging chemical and biological synthesis

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