“…Endocrine-disrupting chemicals (EDCs) can inadvertently cause adverse health effects, such as reproductive pathologies, metabolic diseases, and cancers. Retinoic acids (RAs) are a class of critical regulators for embryo development, playing crucial roles in biological processes that contribute to immune function, hematopoiesis, reproduction, vision, and embryonic patterning and also control cellular differentiation in vertebrates. , In addition to natural RAs and their various oxidative metabolites, several chemicals have recently been reported to have RA disrupting effects (a class of endocrine-disrupting effects), including some phenolic chemicals, parabens, organochlorine pesticides, and organophosphate flame retardants, which have diverse chemical structures and are widely distributed in the environment. − Both RA agonists and antagonists have the potential to harm the organism, with a particularly high risk of causing developmental abnormalities, and therefore, human exposure to such RA-active chemicals is of concern. , This has prompted the evaluation of RA agonistic and antagonistic activities in aquatic environments such as lakes, rivers, and wastewater treatment plants (WWTPs). − However, conventional instrumental analysis technologies, such as high-performance liquid chromatography and spectrophotometry, have disadvantages of being time-consuming and complicated to operate; especially, they cannot screen compounds that have not been previously recognized . Therefore, as with most EDCs, developing a simple and reliable method for continuous monitoring of RA-active chemicals is in high demand and yet remains a significant challenge.…”