1991
DOI: 10.1016/0378-4347(91)80370-r
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Simultaneous assay of the activities of two key enzymes in cholesterol metabolism by gas chromatography—mass spectrometry

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Cited by 32 publications
(16 citation statements)
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“…5␤-Cholestane-3␣,7␣,12␣,24R,25-pentol and 5␤-cholestane-3␣,7␣,12␣,24S,25-pentol were prepared from 5␤-cholestane-3␣,7␣,12␣,25-tetrol by the method of Hoshita (24). [25,26,26,26,27,27 (27) were prepared by previously described methods. Troleandomycin and 6␤-hydroxytestosterone were purchased from Sigma.…”
Section: Methodsmentioning
confidence: 99%
“…5␤-Cholestane-3␣,7␣,12␣,24R,25-pentol and 5␤-cholestane-3␣,7␣,12␣,24S,25-pentol were prepared from 5␤-cholestane-3␣,7␣,12␣,25-tetrol by the method of Hoshita (24). [25,26,26,26,27,27 (27) were prepared by previously described methods. Troleandomycin and 6␤-hydroxytestosterone were purchased from Sigma.…”
Section: Methodsmentioning
confidence: 99%
“…Gas liquid chromatography-mass spectrometry (GC-MS) with selected-ion monitoring (SIM) was performed using a JMS-SX102 instrument equipped with a JMA DA-6000 data-processing system (JEOL, Tokyo, Japan). The accelerating voltage was 10 kV, the ionization energy was 70 eV, the trap current was 300 A, and the mass spectral resolution was about 10,000 (38). An Ultra Performance capillary column (25 m ϫ 0.32 mm id) coated with methylsilicone (Agilent Technologies, DE) was used at a flow rate of helium carrier gas of 1.0 ml/min.…”
Section: Oxysterol Analysismentioning
confidence: 99%
“…of the microanalysis of endogenous substances in biological specimens by GC-MS. As described in our previous investigations (7,10,11), MVA was lactonized easily to MVL and the resulting MVL was converted to the corresponding alkylamides with the primary alkylamines under mild conditions without any catalysts. This amidation via MVL from MVA was a characteristic reaction for g-hydroxy fatty acids such as MVA, and the resulting alkylamides were further converted to the final derivatives suitable for the detection of ionization.…”
Section: Discussionmentioning
confidence: 90%
“…Different quantities (5,10,25,50, and 100 mg of protein) of normal rat liver microsomes were used for the enzyme assay, and extraction and quantification of MVL were conducted as described in Materials and Methods. As shown in Fig.…”
Section: Application To a Microscale Assaymentioning
confidence: 99%