Simultaneous assay of pigments, carbohydrates, proteins and lipids in microalgae

Chen, Yimin; Vaidyanathan, Seetharaman

doi:10.1016/j.aca.2013.03.005

Cited by 139 publications

(64 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The protein content in the microalgae samples was estimated using a modified Micro biuret method [43] with some modification. One mL of 0.5 M NaOH aqueous solution was added to 10 mg of sample and extraction was carried out at 80 °C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth Medium

Safafar

Hass²,

Møller³

et al. 2016

Marine Drugs

View full text Add to dashboard Cite

Nannochloropsis salina was grown on a mixture of standard growth media and pre-gasified industrial process water representing effluent from a local biogas plant. The study aimed to investigate the effects of enriched growth media and cultivation time on nutritional composition of Nannochloropsis salina biomass, with a focus on eicosapentaenoic acid (EPA). Variations in fatty acid composition, lipids, protein, amino acids, tocopherols and pigments were studied and results compared to algae cultivated on F/2 media as reference. Mixed growth media and process water enhanced the nutritional quality of Nannochloropsis salina in laboratory scale when compared to algae cultivated in standard F/2 medium. Data from laboratory scale translated to the large scale using a 4000 L flat panel photo-bioreactor system. The algae growth rate in winter conditions in Denmark was slow, but results revealed that large-scale cultivation of Nannochloropsis salina at these conditions could improve the nutritional properties such as EPA, tocopherol, protein and carotenoids compared to laboratory-scale cultivated microalgae. EPA reached 44.2% ± 2.30% of total fatty acids, and α-tocopherol reached 431 ± 28 µg/g of biomass dry weight after 21 days of cultivation. Variations in chemical compositions of Nannochloropsis salina were studied during the course of cultivation. Nannochloropsis salina can be presented as a good candidate for winter time cultivation in Denmark. The resulting biomass is a rich source of EPA and also a good source of protein (amino acids), tocopherols and carotenoids for potential use in aquaculture feed industry.

show abstract

Section: Methodsmentioning

confidence: 99%

High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth Medium

Safafar

Hass²,

Møller³

et al. 2016

Marine Drugs

View full text Add to dashboard Cite

show abstract

“…The content of protein in control was higher than that of Co enrichment treatment, where the highest values were 6.2% and 4.2% respectively at day 4. Changes in 12/23 protein can reflect the rate of metabolic activity in actively growing cells (Chen and Vaidyanathan, 2013). Both of the contents carbohydrates and proteins were lower in Co enrichment treatment compared with control, meanwhile the hydrocarbon content was high.…”

Section: Effect Of Cobalt Enrichment On B Braunii Carbohydrate and Pmentioning

confidence: 98%

Effect of cobalt enrichment on growth and hydrocarbon accumulation of Botryococcus braunii with immobilized biofilm attached cultivation

Cheng

Wang

Liu

2015

Bioresource Technology

View full text Add to dashboard Cite

The effects of cobalt enrichment on the growth and hydrocarbon accumulation were studied with biofilm attached cultivation. Under biofilm attached cultivation conditions, the microalga Botryococcus braunii survived high concentration of cobalt (50× normal level). The crude hydrocarbon content as well as the long C chain component (C31) increased under Co enrichment treatment indicating the activity of key enzyme that catalyze hydrocarbon synthesis might be enhanced by Co enrichment. The reduced carbohydrate and protein contents accompanied by increased hydrocarbon content for Co enrichment treatment indicating the Co was also an effective factor that controls the carbon flow of B. braunii. Under Co enrichment treatment, totally 1473.9 μmol of Co element was consumed to produce one gram of algal biomass, indicating this attached cultivation method is high efficient in heavy metal elements removal.

show abstract

“…[29]. Carbohydrates and proteins can account for a significant portion of microalgal biomass-as high as 24%-33% carbohydrates and 36%-44% protein in Nannochloropsis gaditana [30], 28% starch, and 35% proteins in Dunaliella salina [31].…”

Section: Discussionmentioning

confidence: 99%

Optimization of One-Step In Situ Transesterification Method for Accurate Quantification of EPA in Nannochloropsis gaditana

et al. 2016

View full text Add to dashboard Cite

Microalgae are a valuable source of lipid feedstocks for biodiesel and valuable omega-3 fatty acids. Nannochloropsis gaditana has emerged as a promising producer of eicosapentaenoic acid (EPA) due to its fast growth rate and high EPA content. In the present study, the fatty acid profile of Nannochloropsis gaditana was found to be naturally high in EPA and devoid of docosahexaenoic acid (DHA), thereby providing an opportunity to maximize the efficacy of EPA production. Using an optimized one-step in situ transesterification method (methanol:biomass = 90 mL/g; HCl 5% by vol.; 70 • C; 1.5 h), the maximum fatty acid methyl ester (FAME) yield of Nannochloropsis gaditana cultivated under rich condition was quantified as 10.04% ± 0.08% by weight with EPA-yields as high as 4.02% ± 0.17% based on dry biomass. The total FAME and EPA yields were 1.58-and 1.23-fold higher separately than that obtained using conventional two-step method (solvent system: methanol and chloroform). This one-step in situ method provides a fast and simple method to measure fatty acid methyl ester (FAME) yields and could serve as a promising method to generate eicosapentaenoic acid methyl ester from microalgae.

show abstract

Simultaneous assay of pigments, carbohydrates, proteins and lipids in microalgae

Cited by 139 publications

References 38 publications

High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth Medium

High-EPA Biomass from Nannochloropsis salina Cultivated in a Flat-Panel Photo-Bioreactor on a Process Water-Enriched Growth Medium

Effect of cobalt enrichment on growth and hydrocarbon accumulation of Botryococcus braunii with immobilized biofilm attached cultivation

Optimization of One-Step In Situ Transesterification Method for Accurate Quantification of EPA in Nannochloropsis gaditana

Contact Info

Product

Resources

About