Simultaneous assay of morphine, morphine-3-glucuronide and morphine-6-glucuronide in human plasma using normal-phase liquid chromatography–tandem mass spectrometry with a silica column and an aqueous organic mobile phase

Weng, Naidong; Lee, Jean W.; Jiang, Xuxian; Wehling, M.; Hulse, James D.; Lin, Patrick

doi:10.1016/s0378-4347(99)00429-6

Cited by 105 publications

(66 citation statements)

References 37 publications

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“…Comparison of ion trap and triple quadrupole MS/MS reveals that the majority of fragment ions is common, even though the intensity pattern is clearly different. In general, the softer conditions of ion trap MS/MS give rise to more high mass fragments, whereas a number of low mass fragments seen in triple quad MS/MS appear in ion trap spectra, if ever, only in MS 3 or MS 4 . However, these limitations should not prevent the setup of useful ESI MS/MS libraries.…”

Section: Resultsmentioning

confidence: 99%

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Electrospray tandem mass spectrometric investigations of morphinans

Raith

Neubert

Poeaknapo

et al. 2003

J. Am. Soc. Mass Spectrom.

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In this study positive ESI tandem mass spectra of the [M ϩ H] ϩ ions of morphinan alkaloids obtained using an ion trap MS were compared with those from a triple quadrupole MS. This allows to assess the differences of the tandem-in-time versus the tandem-in-space principle, often hampering the development of ESI MS/MS libraries. Fragmentation pathways and possible fragment ion structures were discussed. In order to obtain elemental composition, accurate mass measurements were performed. According to the MS/MS fragmentation pathway, the investigated compounds can be grouped into 4 subsets: (1) morphine and codeine, (2) morphinone, codeinone, and neopinone, (3) thebaine and oripavine, (4) -7] or CE/ESI-MS [8,9], mostly from a forensic point of view. Frequently fragmentation by CID is described and applied as LC/MS/MS. Two reviews highlight the field [10,11]. Some systematic approaches to the setup of libraries have been undertaken, including data for morphine and codeine [12][13][14]. However, none of these studies compared data of ion trap and triple quad systems. Most of the morphinan type substances have been investigated before by electron impact ionization MS [15][16][17]. Tandem MS instrumentation and the principles of operation have been extensively discussed in the literature [18]. In contrast to magnetic sector instruments, triple quadrupole, quadrupole ion trap, and quadrupole time-of-flight are all considered to perform low collision energy fragmentation. Nevertheless, the obtained tandem mass spectra can look remarkably different. Although the structure elucidation potential of CID was early recognized [19], the setup of ESI-MS/MS libraries was hampered by lacking reproducibility. This article provides comparable ion trap and triple quadrupole MS/MS data of all discussed substances. Accurate mass data of selected morphinans were obtained by quadrupole time-of-flight and FT-ICR mass spectrometry, respectively.

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Section: Resultsmentioning

confidence: 99%

“…A number of studies reported on identification and quantification of morphine and related metabolites in biological samples by flowinjection ESI-MS [1], LC/ESI-MS [1][2][3][4][5][6][7] or CE/ESI-MS [8,9], mostly from a forensic point of view. Frequently fragmentation by CID is described and applied as LC/MS/MS.…”

mentioning

confidence: 99%

Electrospray tandem mass spectrometric investigations of morphinans

Raith

Neubert

Poeaknapo

et al. 2003

J. Am. Soc. Mass Spectrom.

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show abstract

“…It is imperative to achieve a good chromatographic separation of conjugates (glucuronides) not just from the solvent peak but also from their parent molecules, otherwise they can deconjugate in MS ion source (ESI, APCI) or in the collision cell (ion channel cross-talk), thereby increasing the signal of the parent, which may cause its false over-estimation (Naidong, Lee et al 1999). This is why Jemal et al suggest to use an incurred plasma sample (plasma after dose which still contains metabolites) during the method development in order to ascertain that the developed method for a drug is not compromised by their presence either by chromatographic co-elution and channel cross-talk or by deconjugation during the sample handling (Jemal, Ouyang et al 2010).…”

Section: Chromatographic Methodsmentioning

confidence: 99%

“…Therefore, HILIC can be used primarily for the increased retention and separation of highly polar analytes. For example, a successful application of HILIC mechanism for morphine glucuronides separation and ionization has been described more than a decade ago (Naidong, Lee et al 1999). The retention mechanism in underivatized silica is not only hydrophilic, but also hydrophobic and ion-exchange, which may cause a mixed-mode retention, and is sometimes difficult to predict (Nguyen and Schug 2008).…”

Section: Zwitter Ionic and Hydrophilic Interaction Chromatography (Himentioning

confidence: 99%

Quantification of Glucuronide Metabolites in Biological Matrices by LC-MS/MS

Trontelj¹

2012

Tandem Mass Spectrometry - Applications and Principles

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“…21 In brief, a solid-phase extraction served to isolate MOR, M3G, M6G and their deuterated internal standards from CSF and plasma. The extract was delivered onto a liquid chromatograph tandem mass spectrometer.…”

Section: Assay Techniquesmentioning

confidence: 99%