Simultaneous assay of 3,4-dihydroxyphenylethylene glycol and norepinephrine in human plasma by high-performance liquid chromatography with electrochemical detection

Howes, L. G.; Miller, Samuel T.; Reid, J.

doi:10.1016/0378-4347(85)80111-0

Cited by 31 publications

(16 citation statements)

References 4 publications

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“…Plasma DHPG is derived only from NE recaptured by sympathetic nerve endings after synaptic release [5][6][7][8]. Therefore, the measurement of both NE and DHPG plasma levels would offer accurate information about sympathetic activities, since these levels change under various physiological and pathological situations [2,5,[9][10][11]. Various catecholamine analytical methods using high performance liquid chromatography (HPLC) with electrochemical detection have been reported [12][13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of human plasma catecholamines by high-performance liquid chromatography with a reversed-phase triacontylsilyl silica column

Machida

Sakaguchi

Kamada

et al. 2006

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Simultaneous analysis of human plasma catecholamines by high-performance liquid chromatography with a reversed-phase triacontylsilyl silica column

Machida

Sakaguchi

Kamada

et al. 2006

Journal of Chromatography B

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“…Total plasma NA levels were determined by a high-performance liquid chromatography-electro chemical detection (HPLC-ED) method with a sensi tivity of 20 pg and an interassay coefficient of varia tion of 4.6% [10]. Plasma [3H]-NA radioactivity was determined in 5-ml samples of plasma following ex traction of [3H]-NA on alumina and purification by HPLC.…”

Section: Methodsmentioning

confidence: 99%

Rise in Plasma Noradrenaline with Age Results from an Increase in Spillover Rate

MacGilchrist¹,

Hawksby²,

Howes³

et al. 1989

Gerontology

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Venous plasma noradrenaline (NA) kinetics, determined using steady-state intravenous infusions of subpressor doses of [³H] NA, were measured in 8 young, 13 middle-aged and 8 elderly subjects. Plasma NA concentrations were 0.71 nmol/l (0.25–0.98) in the young, 1.90 nmol/l (0.98–3.65) in the middle-aged and 3.03 nmol/l (1.15–3.85) in the elderly (young vs. elderly, p < 0.001). NA spillover rates were 2.07 nmol/l/m² (1.05–4.91) in the young, 3.91 nmol/l/m² (1.62–9.44) in the middle-aged and 7.57 nmol/l/m² (4.38–24.0) in the elderly (young vs. elderly, p < 0.001). Plasma NA clearance was similar in young and elderly subjects. NA spillover was positively correlated with age (r = 0.58; p < 0.01) and was not independently related to blood pressure. The rise in plasma NA with age results from an increase in spillover rate rather than any alteration in clearance.

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“…At frequent intervals (see Figure 1) venous blood was aspirated into chilled syringes, centrifuged at 40 C and the plasma stored at -20°C for later assay for adrenaline by an h.p.l.c. method employing electrochemical detection (Howes et al, 1985).…”

Section: Introductionmentioning

confidence: 99%

Salbutamol induced hypokalaemia: the effect of theophylline alone and in combination with adrenaline.

Whyte

Reid

Addis

et al. 1988

Brit J Clinical Pharma

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1 We have previously shown that salbutamol induced hypokalaemia, like adrenaline induced hypokalaemia, is the result of stimulation of a membrane bound 132-adrenoreceptor linked to Na+/K+ ATPase. We have also demonstrated that adrenaline induced hypokalaemia is potentiated by therapeutic concentrations of theophylline. 2 In a single-blind study of 14 normal volunteers, we infused salbutamol in doses used in clinical practice and examined the effects of the addition of theophylline alone or combined with (-)-adrenaline on plasma potassium levels, heart rate and blood pressure. The combinations studied were (i) salbutamol + vehicle control adrenaline infusion + placebo theophylline; (ii) salbutamol + vehicle control adrenaline infusion + theophylline; (iii) salbutamol + adrenaline + theophylline. 3 In a randomised, balanced placebo controlled design oral slow release theophylline or placebo was given for 9 days. Subjects were studied twice on the active limb (days 7 and 9) and once on the placebo limb (day 9) and the procedure was identical on each of the 3 study days except for the solutions administered. 4 Theophylline increased salbutamol induced hypokalaemia and in some individuals profound hypokalaemia (< 2.5 mmol 1-1) was observed with these relatively low doses of salbutamol and theophylline. Adrenaline did not further increase the magnitude of the fall in potassium observed. Combining theophylline with salbutamol increased the tachycardia resulting from the salbutamol infusion. Salbutamol infusion caused a fall in diastolic and rise in systolic blood pressure on all 3 study days and this was not altered by either theophylline or adrenaline alone or together. 5 We conclude that theophylline significantly increases salbutamol induced hypokalaemia and tachycardia and that the addition of adrenaline does not further increase hypokalaemia. Intensive bronchodilator therapy with these two agents in acutely ill, hypoxic patients with asthma or chronic obstructive lung disease may increase the risk of serious cardiac arrhythmias secondary to hypokalaemia.

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Simultaneous assay of 3,4-dihydroxyphenylethylene glycol and norepinephrine in human plasma by high-performance liquid chromatography with electrochemical detection

Cited by 31 publications

References 4 publications

Simultaneous analysis of human plasma catecholamines by high-performance liquid chromatography with a reversed-phase triacontylsilyl silica column

Simultaneous analysis of human plasma catecholamines by high-performance liquid chromatography with a reversed-phase triacontylsilyl silica column

Rise in Plasma Noradrenaline with Age Results from an Increase in Spillover Rate

Salbutamol induced hypokalaemia: the effect of theophylline alone and in combination with adrenaline.

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