Simultaneous and sequential influence of metabolite complexes of Lactobacillus rhamnosus and Saccharomyces boulardii and antibiotics against poly-resistant Gram-negative bacteria

Abstract

For the first time the poly-resistant strains of Gram-negative microorganisms were studied for the sensitivity to combined simultaneous and sequential influence of metabolic complexes of Lactobacillus rhamnosus GG and Saccharomyces boulardii, obtained by the author’s method without using the growth media, with antibiotics. The synergic activity of antibacterial preparations and metabolic complexes of L. rhamnosus GG and S. boulardii were studied using modified disk-diffusive method of Kirby-Bauer. During the sequential method of testing (at first the microorganisms were incubated with structural components and metabolites, then their sensitivity to the antibacterial preparations was determined), we observed increase in the diameters of the zones of growth inhibition of Pseudomonas aeruginosa PR to the typical antibiotics (gentamicin, amіcyl, ciprofloxacin, сefotaxime) and non-typical (lincomycin, levomycetin) depending on the tested combinations. Acinetobacter baumannii PR exhibited lower susceptibility: growth inhibition was seen for the combination with ciprofloxacin, сefotaxime, levomycetin. Susceptibility of Lelliottia amnigena (Enterobacter amnigenus) PR increased to levofloxacin, lincomycin. The zones of growth inhibition of Klebsiella pneumoniae PR increased to gentamicin, amіcyl, tetracycline, сeftriaxone. Maximum efficiency was determined during sequential combination of antibiotics with separate metabolic complexes of L. rhamnosus and S. boulardii, and also their combination (to 15.2, 20.2 and 15.4 mm respectively) compared with their simultaneous use (to 12.2, 15.2 and 13.0 mm respectively) for all the tested poly-resistant pathogens, regardless of the mechanism of action of antibacterial preparation. Metabolic complexes of L. rhamnosus GG and S. boulardii, due to increase in the susceptibility of microorganisms, can decrease the therapeutic concentration of antibiotic, slow the probability of the development of resistance of microorganisms, and are therefore promising candidates for developing “accompanying medications” to antibiotics and antimicrobial preparations of new generation.

Minimum Inhibitory and Bactericidal Concentrations of Antibacterial Drugs Separately and Together With Metabolic Complexes of Lactobacillus Rhamnosus Gg and Saccharomyces Boulardii

“…It was difficult to isolate these pathogens during bacteriological studies (S. aureus was isolated predominantly) 22,23 . These results prove the potential for constructing based on metabolic complexes of lactobacilli and saccharomycetes of "accompaniments-preparations" to antibiotics for the treatment of wound infections 24,25,26 . Resistant to ciprofloxacin E. faecalis showed moderate resistance to the combined use of the antibiotic with the metabolite complex ML, despite a decrease in MIC by only half.…”

Antimicrobial and Anti-Biofilm Activity of Polymyxin E Alone and in Combination with Probiotic Strains of Bacillus subtilis KATMIRA1933 and Bacillus amyloliquefaciens B-1895 against Clinical Isolates of Selected Acinetobacter spp.: A Preliminary Study

“…Other studies have also evaluated antimicrobial combinations of the CFS of probiotic strains with antibiotic discs. For example, Isayenko et al [10] found an increase in the diameter of the zones of inhibition for A. baumannii when antibiotics were combined with the metabolite complexes of Lactobacillus rhamnosus (re-classified as Lacticaseibacillus rhamnosus) and Saccharomyces boulardii using the modified disk-diffusion method. Using the same method, in a recent study, Algburi et al [41] reported on the complementary activity of cefotaxime combined with the CFS of B. subtilis KATMIRA1933 and B. amyloliquefaciens B-1895 against methicillin-resistant Staphylococcus aureus.…”

“…Therefore, effective approaches using antimicrobial combinations are urgently required as alternative and safe strategies to combat bacterial resistance to antibiotics. One of the suggested methods is using probiotics and their metabolites as antimicrobial substances in combination with conventional antibiotics to increase the sensitivity of pathogenic strains [10].…”

“…The combination of polymyxin E with other antibiotics, which is widely used by physicians in critical patients, is reported to have a synergistic effect [6]. These combinations have several advantages, such as (i) using lower concentrations of antimicrobials with greater activity, (ii) reducing their cost, and (iii) limiting toxic side effects (nephrotoxicity and neurotoxicity) [10]. This study aimed to evaluate, in vitro, the antibacterial and anti-biofilm activities of the tested probiotic bacilli and their cell-free supernatants (CFS) alone and in combination with polymyxin E against selected Acinetobacter spp.…”

Дослідження Впливу Поживних Субстратів, Які Містять Біологічно Активні Речовини Бактерій, На Окремі Ознаки Та Фактори Патогенності Мікроорганізмів

“…Чималу увагу приділяють проблемам антибіотикорезистентності та біоплівкоутворення бактерій, а також ролі метаболітів мікроорганізмів в цьому напряму [5][6][7][8]. Досі актуальні наукові дослідження у сфері інфекційних хвороб, спричинених стійкими до антибактеріальних препаратів збудниками, й участі похідних бактерій у зазначеному [9][10][11] Методи дослідження. Приготування інокулятів мікроорганізмів для дезінтеграції.…”