Simultaneous analysis of urinary total 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol, N′-nitrosonornicotine, and cotinine by liquid chromatography-tandem mass-spectrometry

Nikam, Sampada S.; Gurjar, Murari; Singhavi, Hitesh; Patil, Anand; Singh, Arjun; Villalta, Peter W.; Chaturvedi, Pankaj; Khariwala, Samir S.; Gota, Vikram; Stepanov, Irina

doi:10.1038/s41598-021-99259-z

Cited by 3 publications

(1 citation statement)

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“…This method has reported LOD, accuracy and precision of 0.05 pmol/mL, 110.9% and 2.9%, respectively, for total NNAL. 56 These will be some of the first estimates of TSNA metabolites in children exposed to ETS from India.…”

Section: Methodsmentioning

confidence: 97%

Protocol for CARES–HAPIN: an ambidirectional cohort study on exposure to environmental tobacco smoke and risk of early childhood caries

Patil,

Puttaswamy,

Cardenas

et al. 2024

BMJ Open

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IntroductionPrenatal and postnatal exposure to environmental tobacco smoke (ETS) has been linked with early childhood caries (ECC), but the specific molecular mechanisms and pathways remain largely unknown. The Caries Risk from exposure to Environmental tobacco Smoke (CARES) within the Household Air Pollution Intervention Network (HAPIN) study aims to establish the association between ETS and ECC by employing epidemiological and novel biomarker-based approaches. Here, we outline the overall design and rationale of the project.Methods and analysisWe will leverage the infrastructure and data from the HAPIN trial (India) to mount the CARES study. In this ambidirectional cohort study, children (n=735, aged: 3–5 years) will undergo ECC examination by a trained dentist using standard criteria and calibrated methods. Structured questionnaires will be used to gather information on sociodemographic variables, dietary habits, oral hygiene, oral health-related quality of life and current exposure to ETS. We will collect non-invasive or minimally invasive biospecimens (i.e., saliva, buccal cells, dried blood spots and urine) from a subset of HAPIN children (n=120) to assess a battery of biomarkers indicative of exposure to ETS, early biological effect and epigenetic modifications. Both self-reported and objective measures of ETS exposure collected longitudinally during in utero and early postnatal periods will be accessed from the HAPIN database. We will apply current science data techniques to assess the association and interrelationships between ETS, ECC, and multiple biomarkers.Ethics and disseminationInformation gathered in this research will be published in peer-reviewed journals and summaries will be shared with the key stakeholders as well as patients and their parents/guardians involved in this study. Sri Ramachandra Institute of Higher Education and Research Ethics Board has approved the study protocol (IEC-NI22/JUL/83/82).Trial registration numberNCT02944682.

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Section: Methodsmentioning

confidence: 97%