Simultaneous analysis of prostanoids using liquid chromatography/high‐field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry

Kapron, James T.; Jin, Wook; Mauriala, Timo; Clark, Patsy; Purves, Randy W.; Bateman, Kevin P.

doi:10.1002/rcm.2505

Cited by 50 publications

(40 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To overcome the limitations of GC-MS and immunoassays for prostaglandin measurements, LC-MS [15][16][17] and LC-MS-MS [18][19][20][21][22][23][24][25][26][27] based methods have evolved as powerful tools for measuring prostaglandins in biological samples because of their high sensitivity, high selectivity and simplicity of sample preparation. Although five of these LC-MS or LC-MS-MS methods have measured PGD 2 [15][16][17][18][19], none have controlled for the inherent chemical instability of PGD 2 which is critical for determining accurate levels and for comparison with the values of other, more stable eicosanoids.…”

Section: Introductionmentioning

confidence: 99%

An improved LC–MS/MS method for the quantification of prostaglandins E2 and D2 production in biological fluids

Cao

Xiao

Park

et al. 2008

Analytical Biochemistry

View full text Add to dashboard Cite

We report an improved LC-MS-MS assay that accurately measures prostaglandins D 2 (PGD 2 ) and E 2 (PGE 2 ) in cell culture supernatants and other biological fluids. The limit of detection for each prostaglandin was 20 pg/mL (0.20 pg; 0.55 fmol on-column), and the inter-day and intra-day coefficients of variation were less than 5%. Both d 4 -PGE 2 and d 4 -PGD 2 were used as surrogate standards to control for differential loss and degradation of the analytes. Stability studies indicated that sample preparation time should be less than 8 h to measure PGD 2 accurately, whereas preparation time did not affect PGE 2 measurement due to its greater stability in biological samples. As an application of the method, PGD 2 and PGE 2 were measured in culture supernatants from A549 cells and RAW 264.7 cells. The human lung alveolar cell line A549 was found to produce PGE 2 but no PGD 2 while the murine macrophage cell line RAW 264.7 produced PGD 2 and only trace amounts of PGE 2 . This direct comparison showed that COX-2 gene expression can lead to differential production of PGD 2 and PGE 2 by epithelial cells and macrophages. Since PGE 2 is anti-asthmatic and PGD 2 is pro-asthmatic, we speculate that the balance of production of these eicosanoids by epithelial cells and macropahges in the lung contributes to the pathogenesis of COPD, bronchiectasis, asthma, and lung cancer.

show abstract

Section: Introductionmentioning

confidence: 99%

An improved LC–MS/MS method for the quantification of prostaglandins E2 and D2 production in biological fluids

Cao

Xiao

Park

et al. 2008

Analytical Biochemistry

View full text Add to dashboard Cite

show abstract

“…Under typical operating conditions, the spread of elimination rates for commonly analyzed ions is reduced from Ͼ5 times in flow-driven to 1.6 times in field-driven FAIMS while the difference in resolving power decreases from ϳ60% to ϳ15%. ield asymmetric waveform ion mobility spectrometry (FAIMS) has emerged as a powerful new analytical technique [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. All IMS methods separate ions using their transport in a gas under the influence of electric field [21]: conventional IMS is based on absolute ion mobility (K) at particular field intensity (E) and FAIMS works with the difference between K at high and low E. So FAIMS is also known as differential mobility spectrometry (DMS) [11,12].…”

mentioning

confidence: 99%

Scaling of the resolving power and sensitivity for planar FAIMS and mobility-based discrimination in flow- and field-driven analyzers

Shvartsburg

Smith

2007

J. Am. Soc. Mass Spectrom.

View full text Add to dashboard Cite

Continuing development of the technology and applications of field asymmetric waveform ion mobility spectrometry (FAIMS) calls for better understanding of its limitations and factors that govern them. While key performance metrics such as resolution and ion transmission have been calculated for specific cases employing numerical simulations, the underlying physical trends remained obscure. Here we determine that the resolving power of planar FAIMS scales as the square root of separation time and sensitivity drops exponentially at the rate controlled by absolute ion mobility and several instrument parameters. A strong dependence of ion transmission on mobility severely discriminates against species with higher mobility, presenting particular problems for analyses of complex mixtures. While the time evolution of resolution and sensitivity is virtually identical in existing FAIMS systems using gas flow and proposed devices driven by electric field, the distributions of separation times are not. The inverse correlation between mobility (and thus diffusion speed) and residence time for ions in field-driven FAIMS greatly reduces the mobility-based discrimination and provides much more uniform separations. Under typical operating conditions, the spread of elimination rates for commonly analyzed ions is reduced from Ͼ5 times in flow-driven to 1.6 times in field-driven FAIMS while the difference in resolving power decreases from ϳ60% to ϳ15%. ield asymmetric waveform ion mobility spectrometry (FAIMS) has emerged as a powerful new analytical technique [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19][20]. All IMS methods separate ions using their transport in a gas under the influence of electric field [21]: conventional IMS is based on absolute ion mobility (K) at particular field intensity (E) and FAIMS works with the difference between K at high and low E. So FAIMS is also known as differential mobility spectrometry (DMS) [11,12]. The value of K for all ions increases or decreases at sufficiently high E, depending on the interaction potentials with gas molecules. As those potentials differ for different ions, the K(E) function is characteristic of the species and, in principle, any two could be distinguished by FAIMS.Separation parameters in FAIMS are largely independent of the ion mass/charge state (m/z) ratio, which renders FAIMS substantially orthogonal to mass spectrometry (MS) and makes FAIMS/MS a powerful method of broad utility. The separation power of FAIMS/MS could be augmented by coupling to conventional IMS providing 2-D separations [17,18] before MS and/or pre-ionization stages such as liquid or gas chromatography (LC [14,16,20] or GC [5,8,9]) or capillary electrophoresis (CE) [10]. The operational simplicity, small size, and low cost make FAIMS attractive for field analyses that require rugged, portable, inexpensive sensors. Recent commercialization of FAIMS technology, alone and in conjunction with LC/MS or GC, has enabled its expansion into proteomics [15][16][17], structural biology [...

show abstract

“…Though we did not use LC or tandem MS in these experiments, it should be borne in mind that FAIMS is compatible with these techniques 33,34 and the capabilities of the analysis could likely be developed even further. Use of FAIMS adds some complexity to the measurements, but the programming of FAIMS can easily be integrated into the automated running of the typical MS system that is intended to normally operate with LC.…”

Section: Resultsmentioning

confidence: 99%

“…29 ESI-FAIMS-MS has been employed advantageously in proteomics applications, 30 analysis of polysaccharides, 31,32 and drug discovery activities. 33 It should be noted that FAIMS separates ions on a timescale which is compatible with those of both liquid chromatography and tandem MS. 33,34 This paper describes the results of a pilot study designed to assess the utility of FAIMS in the analysis of triazines and associated metabolites. Special emphasis was placed on defining the analytical character of ESI-FAIMS-MS compared with direct ESI-MS for these analytes.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Triazines and Associated Metabolites with Electrospray Ionization Field-Asymmetric Ion Mobility Spectrometry/Mass Spectrometry

et al. 2008

View full text Add to dashboard Cite

Triazines comprise an important pollutant class owing to continued use in certain countries, and owing to strong environmental persistence that leads to problems even in countries like Sweden where the use of triazines has been prohibited for some years. We investigated mass-selective detection for analysis of triazines. More specifically, we studied the background reduction and sensitivity enhancement that result from the use of a new interface technique, fieldasymmetric ion mobility spectrometry (FAIMS), in conjunction with electrospray ionization ion-trap mass spectrometry. This technique allows for ion sorting and discrimination against the considerable "chemical noise", nonspecific cluster and fragment ions, which are typically generated in electrospray ionization. This paper presents results of a pilot study of triazines and some metabolites in ideal solvents. Our long-range goal is automated analysis with mass-selective detection coupled to membrane-based sample cleanup and enrichment for additional enhancement in sensitivity.

show abstract

Simultaneous analysis of prostanoids using liquid chromatography/high‐field asymmetric waveform ion mobility spectrometry/tandem mass spectrometry

Cited by 50 publications

References 26 publications

An improved LC–MS/MS method for the quantification of prostaglandins E2 and D2 production in biological fluids

An improved LC–MS/MS method for the quantification of prostaglandins E2 and D2 production in biological fluids

Scaling of the resolving power and sensitivity for planar FAIMS and mobility-based discrimination in flow- and field-driven analyzers

Analysis of Triazines and Associated Metabolites with Electrospray Ionization Field-Asymmetric Ion Mobility Spectrometry/Mass Spectrometry

Contact Info

Product

Resources

About