Simultaneous analysis of prompt and delayed chlorophyll a fluorescence in leaves during the induction period of dark to light adaptation

Correlations between delayed fluorescence of chlorophyll, metabolism and yield of plants. II. Influence of moisture of leaf and temperature condition on delayed fluorescence of leaves

“…It was previously assumed that the time course of DF after the maximum (Figure 1) is possibly influenced by a number of dark photosynthetic processes (directly or indirectly modifying the characteristics of PSII) [13,14]. The decay of induction curve was generally explained by a decrease in the membrane energization as a result of secondary cation efflux from thylakoids [13,14]. Our results showed that half time of I decay-τ describe the level of interaction LR with dark processes of plants [10].…”

“…DF transients have been shown to be driven by many mechanisms, including electrical and pH gradients [11], acceptor availability, donor availability [12], and redox state of the oxygen-evolving system [13,14]. The delayed fluorescence induction curve, registered in a several minutes time period, encompasses two major phases-a fast one, taking place in the first second of induction, and a slow one, that can last for minutes (Figure 1).…”

“…The delayed fluorescence induction curve, registered in a several minutes time period, encompasses two major phases-a fast one, taking place in the first second of induction, and a slow one, that can last for minutes (Figure 1). Each phase is shaped by several processes running at the same time [13,14]. Those initial phases of the induction which many authors attribute to the changes of the electrical potential, depending on the size of ECP on a membrane on the state of the PSII reaction center, since the light induced potential difference and the proton gradient across the thylakoid membrane are thought to reduce the amount of activation energy necessary for the back reaction between QA -and Z + to occur and on the other hand, the I level follows the extent of the variable component of fluorescence and has been suggested to be proportional to the amount of oxidized QA at the beginning of illumination [11,13,14], as well as the concentration of active centers of the PSII [11].…”

“…Each phase is shaped by several processes running at the same time [13,14]. Those initial phases of the induction which many authors attribute to the changes of the electrical potential, depending on the size of ECP on a membrane on the state of the PSII reaction center, since the light induced potential difference and the proton gradient across the thylakoid membrane are thought to reduce the amount of activation energy necessary for the back reaction between QA -and Z + to occur and on the other hand, the I level follows the extent of the variable component of fluorescence and has been suggested to be proportional to the amount of oxidized QA at the beginning of illumination [11,13,14], as well as the concentration of active centers of the PSII [11]. It was previously assumed that the time course of DF after the maximum (Figure 1) is possibly influenced by a number of dark photosynthetic processes (directly or indirectly modifying the characteristics of PSII) [13,14].…”

Photosynthesis in Microalgae as Measured with Delayed Fluorescence Technique

“…Delayed fluorescence originates from the repopulation of excited states of chlorophyll from the stored energy after charge separation (Jursinic, 1986), whereas prompt fluorescence reflects the radiative de-excitation of excited chlorophyll molecules before charge separation. This is why delayed and prompt fluorescence contain information about different fundamental processes of the photosynthetic apparatus (Goltsev et al, 2003). An important feature of DF is that it is emitted only by a functionally active chlorophyll -in other words, when photosynthesis is active (Bertsch, 1962).…”