Simultaneous analysis of monoterpenes and diterpenoids of conifer oleoresin

Abstract

A simple and rapid procedure for the simultaneous determination of monoterpenes and diterpenoid resin acids of conifer stem oleoresin is described; it involves tissue extraction with methyl fert-butyl ether and methylation of the resin acids with CHzNz. The resin acid methyl esters and the monoterpenes are then analysed in a single gas chromatographic step. Enantiomer composition of the major monoterpene constituents is determined, after preliminary purification by thin layer chromatography, by capillary gas chromatography on a cyclodextrin column. The method is applicable to conifer species of high resin content (lodgepole pine) and low resin content (grand fir).

Methyl Jasmonate Induces Traumatic Resin Ducts, Terpenoid Resin Biosynthesis, and Terpenoid Accumulation in Developing Xylem of Norway Spruce Stems

“…Extraction of terpene constituents was modified from Lewinsohn et al (1993). All steps of this procedure were carried out in 2-mL vials (glass with a teflon-coated screw cap, Hewlett-Packard, Palo Alto, CA).…”

Selective reagent ion-time of flight-mass spectrometry study of six common monoterpenes

“…They are the key compounds of plant oleoresin directly participating in plant defense mechanism [1,2], they give signals to the insects associated with plants [3,4], give fruity flavor to our food and beverages [5], they are even present in human breath indicating metabolic state of some organs [6], and they affect our climate globally [7,8].…”

Terpenoid-based defenses in conifers: cDNA cloning, characterization, and functional expression of wound-inducible ( E )-α-bisabolene synthase from grand fir ( Abies grandis )

“…The turpentine fraction of conifer oleoresin may contain up to 30 different monoterpenes (4) and an even larger number of sesquiterpenes (5). These volatiles are toxic to bark beetles (Scolytidae) and their pathogenic fungal symbionts and also furnish the solvent for diterpene resin acids that harden to form a mechanical barrier to seal wound sites upon evaporation of resin volatiles (6).…”

“…The medium was acidified by addition of HCl to 50 mM and then was extracted with diethyl ether. The ether extract was passed over a 0.5-ml column of MgSO 4 and then concentrated for analysis. The cells were frozen in liquid N 2 , ground to a fine powder, suspended in 10 ml of 50 mM HCl, and then extracted with ether; the extract was then prepared as before.…”