Simultaneous analysis of methylprednisolone, methylprednisolone succinate, and endogenous corticosterone in rat plasma

Mehvar, Reza; Dann, Roger O; Hoganson, Dean A

doi:10.1016/s0731-7085(00)00253-3

Cited by 26 publications

(23 citation statements)

References 7 publications

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“…Several examples of the use of surrogate matrices for quantitation of exogenous and endogenous molecules have been reported. [28][29][30][31][32][33] We chose rabbit serum because initial observations indicated no interferences with either human or mouse hepcidin detection. Due to the difficulty of synthesizing human and mouse hepcidin, we were not able to use a stablelabeled internal standard that would have been preferred for development of this mass spectrometry-based assay.…”

Section: Resultsmentioning

confidence: 99%

Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry

Murphy¹,

Witcher

Luan

et al. 2007

Blood

149

131

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The hepatic peptide hormone hepcidin is considered the central regulator of iron metabolism. Characterizing the circulating levels of this peptide is critical to understanding its role in the development of clinically relevant syndromes, such as anemia of inflammation/chronic disease, and may provide insight into potential clinical interventions. While quantitative methods have been published for the determination of urinary hepcidin and serum prohepcidin, no definitive methods have been published for the determination of hepcidin in serum. In this report, we describe a quantitative method for the determination of both human and mouse hepcidin in serum and plasma. The method employs protein precipitation and solid-phase extraction followed by liquid chromatographic separation and tandem mass spectrometry detection. The method has a quantitative range of 0.25 ng/mL to 500 ng/mL serum for mouse hepcidin and 1 ng/mL to 500 ng/mL serum for human hepcidin. The method uses small sample volumes (50 L for mice and 100 L for humans) and 96-well formats for rapid sample processing. The method was used to establish baseline serum and plasma concentrations of hepcidin in normal C57Bl/6 mice and healthy human volunteers. (Blood.

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Section: Resultsmentioning

confidence: 99%

Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry

Murphy¹,

Witcher

Luan

et al. 2007

Blood

149

131

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show abstract

“…O método proposto para este estudo conseguiu atingir 0,02 µg mL -1 e 0,04 µg mL -1 para os limites de detecção e para o de quantificação, respectivamente. Nossos resultados mostram-se melhores que os apresentados por [26], onde o limite de detecção e quantificação foram de 0,1 µg mL -1 e 0,2 µg mL -1 , respectivamente.Vale ressaltar que a metodologia proposta no presente trabalho não necessita do pré-tratamento da amostra, fato que pode ser imprescindível em análises de substâncias bioativas presentes em fluidos biológicos.…”

Section: Resultsunclassified

“…Várias técnicas para a determinação de corticosteróides em fluidos biológicos necessitam do pré-tratamento da amostra [6,9,19,26,34,35,36,37,38,39,40,41] seja através de métodos de extração em fase sólida ou extração por solventes (diclorometano, acetato de etila, metanol, entre outros). Vale ressaltar que o pré-tratamento da amostra pode induzir a alguns problemas, como: o tempo dispensado no processo, manuseio laborioso, erros de manipulação, contaminação por outros agentes, alto custo, decomposição de alguns compostos, entre outros.…”

Section: Corticosterona Acetato De Hidrocortisona Acetato De Dexametaunclassified

Desenvolvimento de um método analítico rápido e eficiente para a determinação de corticosteróides plasmáticos por injeção direta em coluna cromatográfica ISRP-C18 por CLAE

2010

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The present work had as aim the development of simple an analytical method, fast efficient and the determination of corticosteroids (cortisone, corticosterone, acetate of hidrocortisone and acetate of dexametasone) in sanguine samples of rats using an isocratic system of liquid chromatography of high efficiency (CLAE) with ultraviolet detention. The methodinvolved direct injection of sanguine sample in a chromatographic column with internal surface of phase reverse (ISRP-C18), using the composed mobile phase for drain plug phosphate pH 4,0: acetonitrile (65:35 v/v). The detention the analytes was obtained using an ultraviolet detector of changeable wave length (Varian Model 2550) adjusted in 240 nm and a 4400 integratorSP Chromaject (Varian Associates, Inc., Sunnyvale, CA, USA). The extraction of the analyte resulted in recovery values between 90% and 108%, and variation coefficient enter 1,1% and 2.5%. The detention and quantification limits of the method were 0,02 and 0,04 μg mL-1, respectively. In conclusion, the analytical method possible the direct injection (on-line) of the sample without previous treatment also presenting some advantages, such as: fast, exactness, precision and specific.

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“…As we know, sample preparation, mainly including extraction and preconcentration, is very important for the determination of MP in plasma prior to LC [13,14], LC/MS [15], LC/MS/MS [16,17], GC [18], or GC/MS [15,19]. Various preparation techniques have been applied for the extraction of MP in plasma, involving liquid-liquid extraction (LLE) [16], solid-phase extraction (SPE) [20] and so on.…”

Section: Introductionmentioning

confidence: 99%

Development of superparamagnetic high-magnetization C18-functionalized magnetic silica nanoparticles as sorbents for enrichment and determination of methylprednisolone in rat plasma by high performance liquid chromatography

Peng

Wang

Zhang

et al. 2010

Analytica Chimica Acta

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Simultaneous analysis of methylprednisolone, methylprednisolone succinate, and endogenous corticosterone in rat plasma

Cited by 26 publications

References 7 publications

Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry

Quantitation of hepcidin from human and mouse serum using liquid chromatography tandem mass spectrometry

Desenvolvimento de um método analítico rápido e eficiente para a determinação de corticosteróides plasmáticos por injeção direta em coluna cromatográfica ISRP-C18 por CLAE

Development of superparamagnetic high-magnetization C18-functionalized magnetic silica nanoparticles as sorbents for enrichment and determination of methylprednisolone in rat plasma by high performance liquid chromatography

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