Simultaneous analysis of cyclophosphamide, doxorubicin and doxorubicinol by liquid chromatography coupled to tandem mass spectrometry

DiFrancesco, Robin; Griggs, Jennifer J.; Donnelly, Julie; DiCenzo, Robert

doi:10.1016/j.jchromb.2007.02.033

Cited by 54 publications

(51 citation statements)

References 21 publications

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“…However, 24 hr room temperature storage led to about 20% loss. Therefore, DXR and DXR-ol samples should be stored in cold as soon as possible, which is consistent with previous reports [11,24].…”

Section: Validation Of the Proposed Methodssupporting

confidence: 88%

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Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection

Ahmed

Kishikawa

Ohyama

et al. 2009

Talanta

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A highly sensitive and selective high-performance liquid chromatography (HPLC) method was developed for the determination of doxorubicin (DXR) and its metabolite doxorubicinol (DXR-ol) in rat plasma. The method was based on photosensitization reaction followed by peroxyoxalate chemiluminescence detection (PO-CL). DXR and DXR-ol that were fluorescent quinones, served as a photosensitizer in presence of a hydrogen atom donor such as ethanol under aerobic conditions to produce hydrogen peroxide. Then the generated hydrogen peroxide and DXR or DXR-ol were monitored through PO-CL reaction by mixing with aryloxalate as a single post-column reagent that enabled highly selective and sensitive determination of DXR and DXR-ol. The separation of DXR and DXR-ol by HPLC was accomplished isocratically on an ODS column within 15 min. The method involves a simple one step protein precipitation by methanol and a sample size of 50-L was sufficient. Besides, it can detect accurately the low plasma concentrations.The detection limits (signal-to-noise ratio = 3) were 4.5 and 3.8 fmol for DXR and DXR-ol, respectively. The percentage recovery was found to be 90.7-102.4 % and the inter-and intra-assay RSD values in rat plasma were 2.5-8.9 %. The method has been successfully used to study pharmacokinetic profiles of DXR and DXR-ol in rats after a single dose of DXR.

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Section: Validation Of the Proposed Methodssupporting

confidence: 88%

“…Some of them include time-consuming solid-phase extraction processes [23][24][25][26], or liquid-liquid extraction procedures [4,12,17,20,28]. The instability of DXR and DXR-ol in plasma also limits the utility of otherwise promising extraction strategies.…”

Section: Doxorubicin (Dxr) Is a Cytotoxic Anthracycline Antibiotic Thmentioning

confidence: 99%

Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection

Ahmed

Kishikawa

Ohyama

et al. 2009

Talanta

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“…From the 35 publications included in this overview, 12 quantify only one main compound [26,[42][43][44][45][46][47][48][49][50][51][52]. Others determine a main compound and its reduced metabolite [27,[53][54][55][56][57][58][59], sometimes together with additional 8 metabolites [24,[60][61][62][63][64][65][66][67]. One method could be applied to each of the four pairs (main compound and respective reduced metabolite) individually [68].…”

Section: Analytes and Concentrations Of Interestmentioning

confidence: 99%

“…Both the use of aqueous perchloric acid and hydrochloric acid solutions, always in the presence of a small amount of organic solvent, has been described [51,57]. Use of the former does not pose a danger as long as no heating and/or solvent evaporation is involved.…”

Section: Deproteinizationmentioning

confidence: 99%

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Maudens

Stove

Lambert

2011

Journal of Chromatography B

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“…7 The amounts of doxorubicinol synthesized by CBR3 V244 and CBR3 M244 were quantified with a validated high-performance liquid chromatography (HPLC) coupled to tandem mass spectrometry (MS) assay (HPLC-MS/MS) as described. 10 Incubation mixtures (1.0 mL) contained 100 mM potassium phosphate buffer, pH 7.4; 200 lM nicotinamide adenine dinucleotide phosphate (NADPH); 500 lM doxorubicin; and enzyme (CBR3 V244 or CBR3 M244). Mixtures were incubated for 5 hours at 37 8C, and reactions were stopped by placing samples on ice.…”

Section: Kinetic Analysismentioning

confidence: 99%

Genetic polymorphisms in the carbonyl reductase 3 gene CBR3 and the NAD(P)H:quinone oxidoreductase 1 gene NQO1 in patients who developed anthracycline‐related congestive heart failure after childhood cancer

Blanco

Leisenring

González-Covarrubias

et al. 2008

Cancer

210

134

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Introduction: Slow channel congenital myasthenic syndrome is a dominant disorder characterized by prolonged acetylcholine receptor ion‐channel activation. Methods: Molecular genetic techniques, electrophysiology, and binding studies in human embryonic kidney (HEK) 293 cells determined mutant function and expression levels. Patient response to treatment was measured by quantitative myasthenic gravis and Medical Research Council grade strength scores. Results: We report an unusual case due to heteroallelic mutations in CHRNE. The slow channel mutation, p.εS278del, is accompanied by a severe low‐expression mutation, p.εR217L, on the second allele. Expression studies and cosegregation of p.εS278del with the disorder in the patient's offspring demonstrate robust expression of the p.εS278del mutation. The patient showed modest benefits from standard treatment with fluoxetine, but there was dramatic improvement when salbutamol was combined with fluoxetine. Conclusions: This case suggests that salbutamol, which is beneficial in some other congenital myasthenic syndromes, might also be considered in addition to fluoxetine in slow channel syndrome. Muscle Nerve, 2013

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Simultaneous analysis of cyclophosphamide, doxorubicin and doxorubicinol by liquid chromatography coupled to tandem mass spectrometry

Cited by 54 publications

References 21 publications

Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection

Selective determination of doxorubicin and doxorubicinol in rat plasma by HPLC with photosensitization reaction followed by chemiluminescence detection

Quantitative liquid chromatographic analysis of anthracyclines in biological fluids

Genetic polymorphisms in the carbonyl reductase 3 gene CBR3 and the NAD(P)H:quinone oxidoreductase 1 gene NQO1 in patients who developed anthracycline‐related congestive heart failure after childhood cancer

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