Simultaneous analysis of carbohydrates and volatile fatty acids by HPLC for monitoring fermentative biohydrogen production

Sá, Lívian Ribeiro Vasconcelos de; Oliveira, Marcone Augusto Leal de; Cammarota, Magali Christe; Matos, A. P. de; Ferreira-Leitão, Viridiana Santana

doi:10.1016/j.ijhydene.2011.08.056

Cited by 60 publications

(18 citation statements)

References 40 publications

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“…YF is a source of phenolic acids, mainly caffeic and chlorogenic acids, which help to reduce oxidative stress, and studies have shown that it has high antioxidant capacity 10–38 . In the present study, the TAC of the plasma was reduced in groups with induced CRC (groups C and CY) and YF treatment was not able to decrease the oxidative damage caused by the carcinogenesis.…”

Section: Discussioncontrasting

confidence: 56%

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Yacón (Smallanthus sonchifolius) prevented inflammation, oxidative stress, and intestinal alterations in an animal model of colorectal carcinogenesis

et al. 2020

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BACKGROUND: Yacón (Smallanthus sonchifolius) roots store carbohydrate in the form of prebiotic fructooligosaccharides (FOS), which improve intestinal health. Yacon has the potential to prevent the intestinal barrier alterations associated with colorectal cancer (CRC). This study aimed to investigate the preventive effects of yacón flour (YF) on alterations promoted by CRC induced by 1,2-dimethylhydrazine in rats. RESULTS: CRC increased tumor necrosis factor alpha levels (group CY = 10.2 ± 0.72; group C = 9.6 ± 1.0; group Y = 5.8 ± 0.54; group S = 5.95 ± 0.6 pg mL −1) and short-chain fatty acid production, and decreased total antioxidant capacity (group CY = 4.7 ± 0.72; group C = 3.3 ± 0.3; group Y = 4.1 ± 0.47; group S = 6.7 ± 0.78 U mL −1). Furthermore, YF treatment reduced intraluminal pH (group CY = 6.45 ± 0.47; group C = 7.65 ± 0.44; group Y = 6.75 ± 0.46; group S = 8.13 ± 0.2), lactulose/mannitol ratio, tumor necrosis factor-alpha (TNF-⊍)/interleukin (IL)-10 ratio, and increased secretory immunoglobulin A (group

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Section: Discussioncontrasting

confidence: 56%

“…The chromatographic analyses were based on the chromatographic conditions developed by De Sá et al 28 . All analyses were performed using a Shimadzu HPLC system (Kyoto, Japan).…”

Section: Methodsmentioning

confidence: 99%

Yacón (Smallanthus sonchifolius) prevented inflammation, oxidative stress, and intestinal alterations in an animal model of colorectal carcinogenesis

et al. 2020

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“…However, fatty acids show weak UV absorption at the wavelength of 210 nm nearby, so the LOD of the method for direct determination of fatty acids without derivatization was only 6.4-9.4 nmol. 19 To increase the sensitivity, fatty acids were usually derivatived by UV 39 or uorescence reagent. 21,31,[40][41][42][43][44][45][46][47] TSTPE was evaluated by comparing with those labeling reagents used for the derivatization of free fatty acids in other reports in term of derivatization condition and detection limit (see Table 4).…”

Section: Comparisons Of the Proposed Methods With The Reported Methodsmentioning

confidence: 99%

“…The frequently-used detector for HPLC is an UVvis and uorescence detector, but most fatty acids show weak natural absorption in the visible or UV regions and no uorescence, so it is of low sensitivity for direct analysis of fatty acids by HPLC. 19 For these reasons, derivatization procedures allowing a chromophore or uorophore to label fatty acids have been widely adopted, which allows fatty acids to be detected by an UV-vis or uorescence detector and increases the sensitivity of the methods. 11,[20][21][22][23] Many pre-column ultraviolet and uorescence labeling reagents have been developed for analysis of the compounds with carboxy groups in the last decades.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of toluene-4-sulfonic acid 2-(2-thiophen-2-yl-phenanthro[9,10-d]imidazol-1-yl)-ethyl ester and its application for sensitive determination of free fatty acids in ginkgo nut and ginkgo leaf by high performance liquid chromatography with fluorescence detection

2018

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“…Analisis high performance liquid chromatography (HPLC) dengan fase gerak 5 mM H 2 SO 4 laju alir 0,6 ml/min, kolom Aminex HPX-87H suhu 30°C dan refractive index detector (RID) digunakan untuk analisis senyawa gula substrat pulp. Detektor photodiode array (PDA) panjang gelombang 210 nm pada sistem HPLC yang sama digunakan untuk analisis asam organik (Cempaka, Aliwarga, Purwo, & Kresnowati, 2014;De Sa, De Oliveira, Cammarota, Matos, & Ferreira-Leitao, 2011;Pereira et al, 2012). Kadar etanol dianalisis menggunakan gas chromatography (GC) dengan kolom HP-5 dan flame ionization detector (FID) secara isokratik (Ho, Zhao, & Fleet, 2015;Lefeber, Gobert, Vrancken, Camu, & De Vuyst, 2011).…”

Section: Analisis Substrat Dan Metabolit Primerunclassified

Aktivitas Mikrob dalam Pulp Biji Kakao (Theobroma cacao L.) Selama Fermentasi dengan Penambahan Ragi Tape

Purwanto¹,

Setyabudi²,

Supriyanto³

2019

J. tanam. industri dan penyegar

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Microbial activities in cocoa pulp decomposition is key in cocoa beans fermentation. Ragi tape is widely used as a source of microbes (starters). The study aimed to investigate the pattern of changes in the number of microbes, substrate degradation activities and primary metabolites production during fermentation with the addition of ragi tape. The study was conducted at the laboratories within the Faculty of Agriculture Technology and Integrated Laboratory of Experiment and Research, UGM from March to October 2018 . Fermentation experiment used 2 kg of fresh cacao beans stored in a plastic box per batch, its temperature was set daily. The amount of microbes was enumerated using total plate count (TPC), whereas substrate degradation and primary metabolites were analyzed using high performance liquid chromatography (HPLC) and gas chromatography (GC). Temperature, pulp and seed pH, and fermentation index were observed. The results showed that adding ragi tape for fermentation generated a maximum amount of yeast, lactic acid bacteria (LAB) and acetic acid bacteria (AAB) of 9.03+0.85; 9.05+0.17; and 9.15+0.89 log cfu/g of cocoa pulp respectively, with substrate degradation in the form of sucrose reduced by 97%, glucose 98.6%, fructose 97%, and citric acid 71% on the third day. Maximum production of primary metabolites of ethanol is 27.84+21.85 mg/g of cocoa pulp, lactic acid 4.18+3.16 mg/g of cocoa pulp and acetic acid 3.38+5.43 mg/g of cocoa pulp. Fermentation with the addition of ragi tape accelerates the process to three days with a fermentation index value of 1.05+0.06 and seed pH of 5.97+0.20.

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Simultaneous analysis of carbohydrates and volatile fatty acids by HPLC for monitoring fermentative biohydrogen production

Cited by 60 publications

References 40 publications

Yacón (Smallanthus sonchifolius) prevented inflammation, oxidative stress, and intestinal alterations in an animal model of colorectal carcinogenesis

Yacón (Smallanthus sonchifolius) prevented inflammation, oxidative stress, and intestinal alterations in an animal model of colorectal carcinogenesis

Synthesis of toluene-4-sulfonic acid 2-(2-thiophen-2-yl-phenanthro[9,10-d]imidazol-1-yl)-ethyl ester and its application for sensitive determination of free fatty acids in ginkgo nut and ginkgo leaf by high performance liquid chromatography with fluorescence detection

Aktivitas Mikrob dalam Pulp Biji Kakao (Theobroma cacao L.) Selama Fermentasi dengan Penambahan Ragi Tape

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