Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MSn

Chen, Sha; Xiang, Yue; Deng, Jiao; Liu, Yanling; Li, Shaohua

doi:10.1371/journal.pone.0062291

Cited by 47 publications

(38 citation statements)

References 29 publications

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“…Chen et al . () analysed the flavonoid composition and content in leaf pulps, leaf veins and leaf stalks of twelve cultivars lotus and found that the leaf pulps had the highest total flavonoid concentration. Lin et al .…”

Section: Discussionmentioning

confidence: 99%

Effects of in vitro digestion on the composition of flavonoids and antioxidant activities of the lotus leaf at different growth stages

Chen

et al. 2018

Int J of Food Sci Tech

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Summary The lotus leaf is rich in phenolic compounds and has good antioxidant activity. The effects of simulated gastric fluid (SGF) and simulated intestinal fluid (SIF) digestions on the active substances and antioxidant activity of the lotus leaf in different growth stages (D25, D45 and D65) were investigated. The total phenolic content of D25 and D45 significantly increased by SGF digestion but not D65 compared to the DW treatment. However, SIF treatment saw markedly increased total phenolic content across all growth stages (D25, D45 and D65). Moreover, the total flavonoid content at all growth stages was increased by SIF digestion compared to SGF treatment. The pepsin and trypsin had different effects on the FRAP and ABTS (2, 2'‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulphonic acid)) antioxidant activities of the lotus leaf. The SGF digestion increased the FRAP antioxidant capacity of the lotus leaf, and SIF digestion increased the ABTS antioxidant capacity of the lotus leaf. The HPLC results showed that the main flavonoid compound in the lotus leaf was isoquercetin. This study provides a theoretical basis for the development and utilisation of the lotus leaf as an inexpensive natural and functional food ingredient.

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Section: Discussionmentioning

confidence: 99%

Effects of in vitro digestion on the composition of flavonoids and antioxidant activities of the lotus leaf at different growth stages

Chen

et al. 2018

Int J of Food Sci Tech

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“…Because the commonly used methods for extraction and separation, i.e., HPLC, UPLC, MS, or other analytical instruments, are more efficient and consume less organic solvents, they have been developed as efficient methods for plant-characteristic-constituents analysis [14-16]. Due to the complexity of the chemical compositions of different plant samples, the traditional integration methods [17] have been unable to simultaneously take into account the difference between content and activity.…”

Section: Introductionmentioning

confidence: 99%

Identification and Comparison of Anti-Inflammatory Ingredients from Different Organs of Lotus Nelumbo by UPLC/Q-TOF and PCA Coupled with a NF-κB Reporter Gene Assay

et al. 2013

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Lotus nelumbo (LN) (Nelumbo nucifera Gaertn.) is an aquatic crop that is widely distributed throughout Asia and India, and various parts of this plant are edible and medicinal. It is noteworthy that different organs of this plant are used in traditional herbal medicine or folk recipes to cure different diseases and to relieve their corresponding symptoms. The compounds that are contained in each organ, which are named based on their chemical compositions, have led to their respective usages. In this work, a strategy was used to identify the difference ingredients and screen for Nuclear-factor-kappaB (NF-κB) inhibitors with anti-inflammatory ability in LN. Seventeen main difference ingredients were compared and identified from 64 samples of 4 different organs by ultra-performance liquid chromatography that was coupled with quadrupole/time of flight mass spectrometry (UPLC/Q-TOF-MS) with principal component analysis (PCA). A luciferase reporter assay system combined with the UPLC/Q-TOF-MS information was applied to screen biologically active substances. Ten NF-κB inhibitors from Lotus plumule (LP) extracts, most of which were isoquinoline alkaloids or flavone C-glycosides, were screened. Heat map results showed that eight of these compounds were abundant in the LP. In conclusion, the LP extracts were considered to have the best anti-inflammatory ability of the four LN organs, and the chemical material basis (CMB) of this biological activity was successfully validated by multivariate statistical analysis and biological research methods.

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“…Because of the remarkable medicinal functions, plants of the genus Gentiana have been a hot topic in phytochemistry and pharmacology since the 1960s and exhibited chemical diversity (Wang et al ., ; Yang et al ., ). Additionally, more research demonstrated this genus is a rich source of iridoids and phenols such as gentiopicroside, swertiamarin, loganic acid, sweroside and mangiferin, which are commonly regarded as major constituents and likely to responsible for the different therapeutic effect of these folk medicines (Chen et al ., Ikeshiro and Tomita, ; Kakuda et al ., ; Ma et al ., ; Xu et al ., , , , ; Yang et al ., ; Zhang et al ., ). Therefore, the multicomponent analysis could be developed as an important approach for the quality evaluation of ethnomedicines from genus Gentiana .…”

Section: Introductionmentioning

confidence: 98%

Simultaneous determination of six index constituents and comparative analysis of four ethnomedicines from genus Gentiana using a UPLC‐UV‐MS method

Pan

Shen

Zhang

et al. 2014

Biomedical Chromatography

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Many species from genus Gentiana (Gentianaceae) have a long history of applications as folk medicines in the world. A simple rapid UPLC-UV-MS/MS method has been developed and validated for the simultaneous determination of six index constituents (gentiopicroside, swertiamarin, loganic acid, sweroside, mangiferin and ferulic acid) from the four ethnomedicines (G. rigescens Franch. ex Hemsl., G. rhodantha Franch. ex Hemsl., G. scabra Bunge and G. farreri Balf. f.). The UPLC analysis was performed on Shim-Pack XR-ODS III (150 × 2.0 mm, 2.2 µm). The mobile phase was consisted of acetonitrile-0.1% formic acid water using gradient elution. The wavelength 242 nm was chose for the four iridoids as well as mangiferin and 320 nm was set for ferulic acid. Mass spectrometry was applied for identification and quantification for analytes with low concentration. All the regression equations revealed a good linear relationship (R(2) > 0.9993). Accuracy and precision were all within the required limits. The chromatogram fingerprints analysis combined with principal component analysis showed the similarity values of the four species were <0.788 while the similarity values of G. scabra Bunge and G. rigescens Franch. ex Hemsl. were >0.993, which provided reasonable foundation for utilization and clinical application of the four ethnomedicines. This developed method appears to be a useful tool for quality control of the four ethnomedicines.

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Simultaneous Analysis of Anthocyanin and Non-Anthocyanin Flavonoid in Various Tissues of Different Lotus (Nelumbo) Cultivars by HPLC-DAD-ESI-MSn

Cited by 47 publications

References 29 publications

Effects of in vitro digestion on the composition of flavonoids and antioxidant activities of the lotus leaf at different growth stages

Effects of in vitro digestion on the composition of flavonoids and antioxidant activities of the lotus leaf at different growth stages

Identification and Comparison of Anti-Inflammatory Ingredients from Different Organs of Lotus Nelumbo by UPLC/Q-TOF and PCA Coupled with a NF-κB Reporter Gene Assay

Simultaneous determination of six index constituents and comparative analysis of four ethnomedicines from genus Gentiana using a UPLC‐UV‐MS method

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