Simulation of the mucosal environment in the re-construction of the synthetic gut microbial ecosystem

Mabwi, Humphrey A.; Hitayezu, Emmanuel; Mauliasari, Intan Rizki; Mwaikono, Kilaza Samson; Yoon, Hye-Kyung; Komba, Erick; Pan, Cheol‐Ho; Hyun, Kwang

doi:10.1016/j.mimet.2021.106351

Cited by 4 publications

(7 citation statements)

References 43 publications

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“…These results of the mucosal microbiota simulation indicate the effectiveness of the gut‐mucosal environment simulation in this study and corroborate previous in vitro 16 and in vivo 30 data revealing differences in mucosal and luminal microbiota. Emodin treatment in the synthetic gut ecosystem decreased relative abundances of two major enterotypes, namely Bacteroides and Prevotella (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Synthetic gut microbial communities were cultured in tubes designed to represent both the mucosal and luminal microbiota. For this purpose, mucin–agar gel was used to simulate the mucosal environment, as previously described 16 . Briefly, the mucin–agar gel was prepared using a combination of porcine type‐III gastric mucin and agar at a 50 g L −1 :30 g L −1 ratio.…”

Section: Methodsmentioning

confidence: 99%

“…However, culturing fecal samples with chemicals of interest may fail to adequately screen the GM for interactions with phytochemicals, as it contains many microbial species that vary between people 15 . In this regard, it could be appropriate to study synthetic GM communities as a bottom‐up approach for co‐culturing gut bacteria with phytochemicals while simulating the mucosal environment 16 …”

Section: Introductionmentioning

confidence: 99%

“…15 In this regard, it could be appropriate to study synthetic GM communities as a bottom-up approach for co-culturing gut bacteria with phytochemicals while simulating the mucosal environment. 16 Despite the benefits of emodin on GM interactions in alleviating kidney-related diseases and more recently in counteracting iodine-induced thyroiditis, previous studies on emodin-GM interactions are still scarce. 17,18 In this study, we tried to determine whether emodin can actually change the gut microbial community through in vitro bacteria cultivation.…”

Section: Introductionmentioning

confidence: 99%

“… 15 In this regard, it could be appropriate to study synthetic GM communities as a bottom‐up approach for co‐culturing gut bacteria with phytochemicals while simulating the mucosal environment. 16 …”

Section: Introductionmentioning

confidence: 99%

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Emodin modulates gut microbial community and triggers intestinal immunity

et al. 2022

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BACKGROUND: The gut microbiota (GM) plays an important role in human health and is being investigated as a possible target for new therapies. Although there are many studies showing that emodin can improve host health, emodin-GM studies are scarce. Here, the effects of emodin on the GM were investigated in vitro and in vivo. RESULTS:In vitro single bacteria cultivation showed that emodin stimulated the growth of beneficial bacteria Akkermansia, Clostridium, Roseburia, and Ruminococcus but inhibited major gut enterotypes (Bacteroides and Prevotella). Microbial community analysis from a synthetic gut microbiome model through co-culture indicated the consistent GM change by emodin. Interestingly, emodin stimulated Clostridium and Ruminococcus (which are related to Roseburia and Faecalibacterium) in a mice experiment and induced anti-inflammatory immune cells, which may correlate with its impact on specific gut bacteria.CONCLUSION: Emodin (i) showed similar GM changes in monoculture, co-culture, and in an in vivo mice experiment and (ii) simulated regulatory T-cell immune responses in vivo. This suggest that emodin may be used to modulate the GM and improve health.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Emodin modulates gut microbial community and triggers intestinal immunity

et al. 2022

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Roseburia intestinalis‐derived extracellular vesicles ameliorate colitis by modulating intestinal barrier, microbiome, and inflammatory responses

Han,

Hwang,

Choi

et al. 2024

J of Extracellular Vesicle

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Inflammatory bowel disease (IBD) is a chronic disorder characterized by recurrent gastrointestinal inflammation, lacking a precise aetiology and definitive cure. The gut microbiome is vital in preventing and treating IBD due to its various physiological functions. In the interplay between the gut microbiome and human health, extracellular vesicles secreted by gut bacteria (BEVs) are key mediators. Herein, we explore the role of Roseburia intestinalis (R)‐derived EVs (R‐EVs) as potent anti‐inflammatory mediators in treating dextran sulfate sodium‐induced colitis. R was selected as an optimal BEV producer for IBD treatment through ANCOM analysis. R‐EVs with a 76 nm diameter were isolated from R using a tangential flow filtration system. Orally administered R‐EVs effectively accumulated in inflamed colonic tissues and increased the abundance of Bifidobacterium on microbial changes, inhibiting colonic inflammation and prompting intestinal recovery. Due to the presence of Ile‐Pro‐Ile in the vesicular structure, R‐EVs reduced the DPP4 activity in inflamed colonic tissue and increased the active GLP‐1, thereby downregulating the NFκB and STAT3 via the PI3K pathway. Our results shed light on the impact of BEVs on intestinal recovery and gut microbiome alteration in treating IBD.

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Gut Microbiota Patterns in Patients with Non-Alcoholic Fatty Liver Disease: A Comprehensive Assessment Using Three Analysis Methods

Korobeinikova,

Zlobovskaya,

Sheptulina

et al. 2023

IJMS

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Non-alcoholic fatty liver disease (NAFLD) is considered the most common chronic liver disease worldwide, affecting nearly 25% of the global adult population. Increasing evidence suggests that functional and compositional changes in the gut microbiota may contribute to the development and promote the progression of NAFLD. 16S rRNA gene next-generation sequencing is widely used to determine specific features of the NAFLD microbiome, but a complex system such as the gut microbiota requires a comprehensive approach. We used three different approaches: MALDI-TOF-MS of bacterial cultures, qPCR, and 16S NGS sequencing, as well as a wide variety of statistical methods to assess the differences in gut microbiota composition between NAFLD patients without significant fibrosis and the control group. The listed methods showed enrichment in Collinsella sp. and Oscillospiraceae for the control samples and enrichment in Lachnospiraceae (and in particular Dorea sp.) and Veillonellaceae in NAFLD. The families, Bifidobacteriaceae, Lactobacillaceae, and Enterococcaceae (particularly Enterococcus faecium and Enterococcus faecalis), were also found to be important taxa for NAFLD microbiome evaluation. Considering individual method observations, an increase in Candida krusei and a decrease in Bacteroides uniformis for NAFLD patients were detected using MALDI-TOF-MS. An increase in Gracilibacteraceae, Chitinophagaceae, Pirellulaceae, Erysipelatoclostridiaceae, Muribaculaceae, and Comamonadaceae, and a decrease in Acidaminococcaceae in NAFLD were observed with 16S NGS, and enrichment in Fusobacterium nucleatum was shown using qPCR analysis. These findings confirm that NAFLD is associated with changes in gut microbiota composition. Further investigations are required to determine the cause-and-effect relationships and the impact of microbiota-derived compounds on the development and progression of NAFLD.

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Simulation of the mucosal environment in the re-construction of the synthetic gut microbial ecosystem

Cited by 4 publications

References 43 publications

Emodin modulates gut microbial community and triggers intestinal immunity

Emodin modulates gut microbial community and triggers intestinal immunity

Roseburia intestinalis‐derived extracellular vesicles ameliorate colitis by modulating intestinal barrier, microbiome, and inflammatory responses

Gut Microbiota Patterns in Patients with Non-Alcoholic Fatty Liver Disease: A Comprehensive Assessment Using Three Analysis Methods

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