Simulation of the Effect of Confinement in Actin Ring Formation

Koudehi, Maral Adeli; Tang, Haosu; Vavylonis, Dimitrios

doi:10.1016/j.bpj.2015.11.725

Cited by 3 publications

(1 citation statement)

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“…Because any future design of a minimal cell‐like system must likely involve compartmentalization, we also verified the functionality of the segregation system in microcompartments, as has previously been done for several protein machineries including actin, the Min system, and MreB . Although the compartmentalization itself apparently did not interfere with the function of the ParMRC system, the performance of the encapsulated system deteriorated more rapidly than in the case of the reactions performed in bulk.…”

Section: Discussionsupporting

confidence: 58%

Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System

et al. 2019

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A biomimetic system capable of replication and segregation of genetic material constitutes an essential component for the future design of a minimal synthetic cell. Here we have used the simple T7 bacteriophage system and the plasmid‐derived ParMRC system to establish in vitro DNA replication and DNA segregation, respectively. These processes were incorporated into biomimetic compartments providing an enclosed reaction space. The functional lifetime of the encapsulated segregation system could be prolonged by equipping it with ATP‐regenerating and oxygen‐scavenging systems. Finally, we showed that DNA replication and segregation processes could be coupled in vitro by using condensed DNA nanoparticles resulting from DNA replication. ParM spindles extended over tens of micrometers and could thus be used for segregation in compartments that are significantly longer than bacterial cell size. Overall, this work demonstrates the successful bottom‐up assembly and coupling of molecular machines that mediate replication and segregation, thus providing an important step towards the development of a fully functional minimal cell.

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Section: Discussionsupporting

confidence: 58%

Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System

et al. 2019

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Mathematical Model of Contractile Ring-Driven Cytokinesis in a Three-Dimensional Domain

Lee

2018

Bull Math Biol

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In this paper, a mathematical model of contractile ring-driven cytokinesis is presented by using both phase-field and immersed-boundary methods in a three-dimensional domain. It is one of the powerful hypotheses that cytokinesis happens driven by the contractile ring; however, there are only few mathematical models following the hypothesis, to the author's knowledge. I consider a hybrid method to model the phenomenon. First, a cell membrane is represented by a zero-contour of a phase-field implicitly because of its topological change. Otherwise, immersed-boundary particles represent a contractile ring explicitly based on the author's previous work. Here, the multi-component (or vector-valued) phase-field equation is considered to avoid the emerging of each cell membrane right after their divisions. Using a convex splitting scheme, the governing equation of the phase-field method has unique solvability. The numerical convergence of contractile ring to cell membrane is proved. Several numerical simulations are performed to validate the proposed model.

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Emergence of Polygonal Shapes in Oil Droplets and Living Cells: The Potential Role of Tensegrity in the Origin of Life

Gordon

Hanczyc

Denkov

et al. 2018

Habitability of the Universe Before Earth

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Simulation of the Effect of Confinement in Actin Ring Formation

Cited by 3 publications

References 0 publications

Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System

Reconstitution and Coupling of DNA Replication and Segregation in a Biomimetic System

Mathematical Model of Contractile Ring-Driven Cytokinesis in a Three-Dimensional Domain

Emergence of Polygonal Shapes in Oil Droplets and Living Cells: The Potential Role of Tensegrity in the Origin of Life

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