Simulation of the breakthrough curves for the adsorption of α-lactalbumin and β-lactoglobulin to SP Sepharose FF cation-exchanger

El‐Sayed, Mayyada M. H.; Chase, Howard A.

doi:10.1016/j.bej.2009.12.017

Cited by 15 publications

(7 citation statements)

References 23 publications

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“…Nevertheless, there was an overshoot when the maximum outlet concentration (C) exceeded the column inlet concentration (C 0 )(C/C 0 > 1). There are several studies which report this overshoot phenomenon during the adsorption of various metals using adsorbents such as biomass-derived seaweed, activated sludge, and chelate resin [15][16][17][18][19]. Similar phenomena have also been reported in gas adsorption by activated carbon [20].…”

Section: Overshoot In the Breakthrough Curve By Beech Sawdustmentioning

confidence: 58%

Mechanism of Cesium Adsorption by Carbonized Rice Hull and Beech Sawdust

Miura

2018

Separations

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This study used the results of breakthrough experiments to elucidate the mechanism of cesium adsorption on carbonized rice hull and beech sawdust. The shape of the breakthrough curves and the substance eluted from the carbonized rice hull and beech sawdust were investigated for various flow speeds and concentrations of the solute through the adsorbent layer. The ratio of the Cs concentration at the column outlet (C) to that at the inlet (C 0 ), C/C 0 , was calculated to evaluate the mechanism. It was found that carbonized rice hull could slowly adsorb Cs as the Cs solution passed through the fixed-bed layer. On the other hand, beech sawdust could rapidly adsorb Cs upon contact with the Cs solution. It was, therefore, suggested that the two materials adsorbed Cs through an ion-exchange reaction with potassium on their respective surfaces. Moreover, the shape of the breakthrough curve of beech sawdust changed as the Cs concentration at the column inlet was varied. This phenomenon was presumably caused by a factor other than the swelling or contraction of the adsorption layer. Further analysis revealed that the breakthrough curve of beech sawdust is indicative of an overshoot phenomenon when cesium was adsorbed by beech sawdust.

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Section: Overshoot In the Breakthrough Curve By Beech Sawdustmentioning

confidence: 58%

Mechanism of Cesium Adsorption by Carbonized Rice Hull and Beech Sawdust

Miura

2018

Separations

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“…El-Sayed and Chase (2010) studied whey proteins fractionation and published several papers on purification of whey α-La and β-Lg using cation exchange chromatography. They show a novel consecutive two-stage separation process was developed to separate α-La and β-Lg from whey concentrate mixtures [ 18 ]. SP-FF column with 0.1 M sodium acetate buffer for column equilibration at pH 3.7 and 0.1 M Tris-HCl for elution at pH 5.2 and pH 7 resulted in high purity and recovery of α-La and β-Lg.…”

Section: Discussionmentioning

confidence: 99%

Lactoferrin Isolation and Hydrolysis from Red Deer (Cervus elaphus) Milk and the Antibacterial Activity of Deer Lactoferrin and Its Hydrolysates

Wang

Bekhit

Mason

et al. 2020

Foods

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Lactoferrin (Lf) and other whey proteins have been isolated from red deer milk for the first time using a three-step anion and cation exchange chromatography protocol. The separated deer Lf was subject to in vitro gastric and duodenal digestions to generate peptides. The purity of the deer Lf and its hydrolysis products were analyzed by SDS-PAGE. The antibacterial activity of the deer Lf and its hydrolysates were investigated and was compared to cow counterpart. Gastric and duodenal digested deer Lf had strong bactericidal activity against E. coli ATCC 25922 with minimum inhibition concentration (MIC) of 280 µM and 402 µM, respectively. These results suggest that deer milk contains bioactive whey proteins and can generate bioactive peptides, which can benefit human health by inhibiting food-borne pathogenic bacteria.

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“…The purity of BLG was 95% in the first stage and 86% in the second one. These authors also simulated the breakthrough curves for the adsorption of ALA and BLG SP Sepharose FF [99] and, for adsorption of WPC, the simple kinetic model accurately predicted the individual breakthrough curves for ALA and BLG after correcting the Langmuir isotherm parameters for BLG using the same factors as proved necessary for pure mixtures.…”

Section: Selective Adsorptionmentioning

confidence: 99%

Application of Ion Exchange and Adsorption Techniques for Separation of Whey Proteins from Bovine Milk

Radosavljević

Stanić-Vučinić

Stojadinovic

et al. 2021

CAC

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Background: The world production of whey was estimated to be more than 200 million tons per year. Although whey is an important source of proteins with high nutritional value and biotechnological importance, it is still considered as a by-product of the dairy industry with low economic value due to low industrial exploitation. There are several challenges in the separation of whey proteins: low concentration, the complexity of the material and similar properties (pI, molecular mass) of some proteins. Methods: A narrative review of all the relevant papers on the present methodologies based on ion-exchange and adsorption principles for isolation of whey proteins, known to the authors, was conducted. Results: Traditional ion-exchange techniques are widely used for the separation and purification of the bovine whey proteins. These methodologies, based on the anion or cation chromatographic procedures, as well as combination of aforementioned techniques are still preferential methods for the isolation of the whey proteins on the laboratory scale. However, more recent research on ion exchange membranes for this purpose has been introduced, with promising potential to be applied on the pilot industrial scale. Newly developed methodologies based either on the ion-exchange separation (for example: simulated moving bed chromatography, expanded bed adsorption, magnetic ion exchangers, etc.) or adsorption (for example: adsorption on hydroxyapatite or activated carbon, or molecular imprinting) are promising approaches for scaling up of the whey proteins’ purification processes. Conclusion: Many procedures based on ion exchange are successfully implemented for separation and purification of whey proteins, providing protein preparations of moderate-to-high yield and satisfactory purity. However, the authors anticipate further development of adsorption-based methodologies for separation of whey proteins by targeting the differences in proteins’ structures rather than targeting the differences in molecular masses and pI. The complex composite multilayered matrices, including also inorganic components, are promising materials for simultaneous exploiting of the differences in the masses, pI and structures of whey proteins for the separation.

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Simulation of the breakthrough curves for the adsorption of α-lactalbumin and β-lactoglobulin to SP Sepharose FF cation-exchanger

Cited by 15 publications

References 23 publications

Mechanism of Cesium Adsorption by Carbonized Rice Hull and Beech Sawdust

Mechanism of Cesium Adsorption by Carbonized Rice Hull and Beech Sawdust

Lactoferrin Isolation and Hydrolysis from Red Deer (Cervus elaphus) Milk and the Antibacterial Activity of Deer Lactoferrin and Its Hydrolysates

Application of Ion Exchange and Adsorption Techniques for Separation of Whey Proteins from Bovine Milk

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