Simulation of Microbial Growth Dynamics Coupled to Nutrient and Oxygen Transport in Porous Media

Abstract

A model for simulating microbial growth‐degradation processes in porous media is developed. It is assumed that the bulk of microorganisms in an aquifer grow in microcolonies attached to matrix surfaces. As developed, the model applies to the growth and decay of aerobic, heterotrophic microorganisms whose growth is limited by lack of a carbon and energy source (substrate), an oxygen source or both simultaneously as described by modified Monod kinetics. Transport of substrate and oxygen in the porous medium is assumed to be governed by advection‐dispersion equations with surface adsorption. A total of five coupled equations result describing substrate and oxygen concentrations in the pore fluid, substrate and oxygen concentrations in the microcolonies and colony density, which is assumed sufficiently small so that aquifer hydraulic conductivity is not diminished. An iterative process involving an Eulerian‐Lagrangian numerical procedure that is highly resistant to numerical dispersion in the presence of small dispersivities is used to solve the overall model, with parameter values selected from the literature or estimated. Results indicate that biodgradation would be expected to have a major effect on contaminant transport when proper conditions for growth exist. For one‐dimensional transport in a column, the most rapid microbial growth always occurred at the influent boundary where oxygen and substrate concentrations were held constant independent of colony density. Anaerobic conditions develop rapidly and aerobic biodegradation ceases if large amounts of substrate are added to the system.

In Situ: Groundwater Bioremediation

“…The simple kinetic models using Monod or Michaelis-Menten functions of 15 years ago are completely inadequate for current bioremediation applications in the terrestrial subsurface. One and two-dimensional models of aerobic biodegradation of organic contaminants in ground water did not appear until quite recently (Molz et al, 1986;Widdowson et al, 1987). These models used advective and dispersive transport coupled with an assumption of microcolonies.…”

Nitrogen transformation and transport modeling in groundwater aquifers

“…The multiple-Monod kinetics (Molz et al, 1986;Widdowson et al, 1988;Kinzelbach et al, 1991;Lindstrom, 1992;Chen et al, 1992;Essaid et al, 1995;MacQuarrie and Sudicky, 1997;Lu et al, 1999) are used for describing the biodegradation reaction processes that involve several solutes. The kinetic rate equations are used to describe all of the major reaction processes in the RT3D.…”

Kinetic modeling of microbially-driven redox chemistry of radionuclides in subsurface environments: coupling transport, microbial metabolism and geochemistry

“…We fin-t.her assume that the microorganisms responsible for biode-sgdation can be roughly divided into six groups (aerobes, denitrifiers, Mn(IV) reducers, Fe(III) reducers, sulfate reducers, and methanogens), each corresponding to an individual degradation pathway. Using the multiple Monod formulation (Molz et al, 1986;Essaid et al, 1995), the degradation rate of organic compound i via thej-th reaction pathway can be expressed by: Ky~and KY= are the half-saturation constants; Pj is the active biom~s Of the~-th group microorganisms; and~characterizes the activity of thej-th group microorganisms.…”