Simulation of giant fibre development in biopsy samples from pig longissimus muscle

Schubert-Schoppmeyer, A.; Fiedler, I.; Nürnberg, G.; Jonas, Ludwig; Ender, K.; Maak, Steffen; Rehfeldt, Charlotte

doi:10.1016/j.meatsci.2008.06.008

Cited by 24 publications

(24 citation statements)

References 30 publications

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“…In accordance with Rahelic and Puac (1980) we observed giant fibres in the Ld muscle that is a white muscle in which fast-twitch glycolytic fibres prevail in number. The ''giant fibre" phenomenon is known to occur in skeletal muscle samples obtained after slaughter and has been described in pigs of different breeds (Fiedler et al 1999;Handel and Stickland 1986) and confirmed in the study of Schubert-Schoppmeyer et al (2008). Our study shows that there are differences between males and females with regard to the size of all three fibre types; namely, the males showed larger fibres than the females (Figs 5 and 6).…”

Section: Discussionsupporting

confidence: 83%

New Insights into Muscle Fibre Types in Casertana Pig

et al. 2010

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Little is known about the Casertana pig. The aim of this study was to evaluate the effect of sex on histochemical and morphometrical characteristics of muscle fibres (myocytes) in this pure breed and to verify the presence of giant fibres as well as vascularity of the muscle. Finally, maximum shortening velocity and isometric tension were measured in single muscle fibres.Sixteen Casertana pigs (8 males, 8 females) from a farm in Campania (Italy) were slaughtered at one year of age. Muscle tissues were obtained from psoas minor, rhomboideus and longissimus dorsi. Myofibres were stained for myosin adenosine triphosphatase, succinic dehydrogenase, and α-amylase-periodic acid schiff. For all fibre types, the area and perimeter were measured. Slowtwitch oxidative fibres, fast-twitch glycolytic fibres and fast-twitch oxidative-glycolytic fibres were histochemically differentiated; an image-analyzing system was used. The results showed significant differences between the sexes in the size of all three fibre types. The psoas minor muscle had a high percentage of slow-twitch oxidative fibres and contained more capillaries per fibre and per mm 2 than rhomboideus and longissimus dorsi, in which fast-twitch glycolytic fibres dominated. The cross-sectional area of all fibre types was larger in longissimus dorsi than in rhomboideus and psoas minor muscles; the giant fibres were present in the longissimus dorsi muscle only. Besides, isometric tension values were higher in fast-twitch glycolytic fibres than in the other ones. Variations in fibre type composition may contribute to meat quality. Giant fibres, myosin adenosine triphosphatase, succinic dehydrogenase, capillaries, shortening velocity, isometric tensionThe Casertana breed is an autochthonous pig breed of ancient origin, now threatened with extinction. In the past it was considered a very fine breed, raised in the Campania region and the surrounding area in South Italy. It was valued for its productive performance particularly regarding the fattening tendency. It is characterized by slow growth and massive accumulation of backfat; its coat is bright black and hairless. The purpose of this study was to complete the knowledge regarding the Casertana pig in order to valorise this breed and to study its meat quality characteristics. Porcine meat quality can be affected by variation in muscle fibre type composition (Depreux 2000). Muscle fibre types may be classified based on enzymatic activity; in this study the following nomenclature was used for myofibre types: FG (fast-contracting with glycolytic metabolism), FOG (fast contracting with glycolytic-oxidative metabolism) and SO (slow-contracting with oxidative metabolism). The aim of this study was to evaluate the effect of sex on the histochemical and morphometric characteristics of muscle fibres on the basis of myosin adenosine triphosphatase (myosin ATPase), succinic dehydrogenase (SDH), to analyse the contractile properties of pig muscle fibres and to determine the presence of giant fibres in the muscles consi...

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Section: Discussionsupporting

confidence: 83%

New Insights into Muscle Fibre Types in Casertana Pig

et al. 2010

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“…However, considering the different publications, these alterations did not generally result in significant drip loss differences. For example, the drip loss values of low and normal pH 45 min p.m. pork in the present study or the studies of Schubert-Schoppmeyer et al (2008) and Zelechowska et al (2012) only showed a difference of 0.67%, 1.03%, 1.02%, respectively (P > 0.05), in comparison with Gil et al (2003), Copenhafer et al (2006) and Werner et al (2010a) (1.5%, 4.8%, 3.3%, respectively, P < 0.05). Further investigations are necessary to clarify the relation between rate of pH decline and WHC/drip loss.…”

Section: Resultscontrasting

confidence: 64%

“…The relation between low pH 45 min p.m. values and meat conductivity was also shown by Schubert-Schoppmeyer et al (2008) and Werner et al (2010a). The faster pH decrease in the low pH group results in pronounced shrinkage and denaturation of the myofibrillar proteins accompanied with accelerated release of water and electrolytes (Scheffler and Gerrard, 2007).…”

Section: Resultsmentioning

confidence: 70%

The relationship of pork longissimus muscle pH to mitochondrial respiratory activities, meat quality and muscle structure

Popp

Wicke

Klein

et al. 2015

Animal

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The way the pH falls post-slaughter has an impact on meat quality. Pork longissimus muscles (n = 48) were sorted in fast-(FG) (n = 15) and normal glycolysing (NG) (n = 33) muscles according to the post-slaughter pH 45 min values (FG < 6.0; NG > 6.0). FG muscles (5.84 ± 0.04) compared with NG muscles (6.27 ± 0.04) were accompanied with higher temperature, electrical conductivity, lightness and yellowness, and reduced grill loss and shear force values ( P < 0.05), but there were no pH-dependent changes of the drip loss and redness results. FG muscles had higher ( P < 0.05) percentages of fast-twitch glycolytic and lower proportions of fast-twitch oxidative and slow-twitch oxidative ( P < 0.05) muscle fibres. The study confirms the relationship of pH value to meat quality and muscle fibre characteristics also showing that pH values have no impact on intrinsic mitochondrial respiratory function.

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“…Besides, we noticed that there were giant fibres, especially in the brown trout of group A. These fibres were found in other fish and mammalians (Acosta et al 2005;Schubert-Schoppmeyer et al 2008) and they were correlated with poor meat quality. Finally, as previously described, the SGR was not significantly affected by the dietary intake of propolis at 10 g propolis·kg -1 diet according to the results of Cuesta et al (2005) and in discordance with Abd-El-Rhman (2009) according to who propolisethanolic -extract and crude propolis evoked the highest specific growth rate.…”

Section: Body Length and Fibre Typementioning

confidence: 67%

Effect of Propolis on the Fish Muscular Development and Histomorphometrical Characteristics

Velotto¹,

Vitale²,

Varricchio

et al. 2010

Acta Vet. Brno

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The aim of our study was to evaluate the effect of propolis on mortality of fish eggs due to mycosis, to study its efficacy on the fish growth rate and to analyze the histochemical and ultrastructural characteristics of muscle fibres. We evaluated the muscular development of fish in two different experimental conditions: traditional feeding and feeding with addition of propolis (groups A and B, respectively). The study was carried out on two species of teleosts bred in Italy, Oncorhynchus mykiss (rainbow trout) and Salmo trutta (brown trout). The use of propolis reduced mortality of fish eggs. The muscles were stained for myosin ATPase and succinic dehydrogenase to evaluate fibre type. The area, maximum and minimum diameter for each fibre type in relation to the body length were measured. The animals fed propolis showed a more rapid muscular growth compared to control fed the standard diet. Fibres were larger in the Salmo trutta than in the Oncorhynchus mykiss in both groups. In fish of A group, myotomal muscle, the presence of giant fibres and index of environmental stress was noticed. This type of study is valuable in order to extend the use of propolis as an antifungal agent and a natural product that can improve fish farming. ATPase and SDH activities, specific growth-rate, ultrastructural analysisEuropean aquaculture has shown a steady increase during the last twenty years, thanks to many initiatives promoted by the European Community to support this sector. But there are still many problems to solve, such as the support of ecocompatible activities, the improvement of animal health and welfare, product quality and consumer health assurance. Propolis is a mixture of various amounts of beeswax and resins collected by the honeybee from plants, particularly from flowers and leaf buds (Krell 1996). The colour of propolis ranges from yellow to dark brown depending on the origin of the resins. Propolis has attracted the researchers' interest in the last decades due to its biological and pharmacological properties, such as immunomodulatory, antitumor, antimicrobial, antiinflammatory, and antioxidant properties (Kanbur et al. 2009). The goal of this study was to analyze, for the first time, the influence of propolis on the fish muscular development and on the structural and ultrastructural characteristics of muscle fibre types. This study opens a new perspective on the investigation of biological properties of propolis, mainly with respect to the influence on the development of the trout and the mortality of fish eggs. Materials and MethodsThe following species of freshwater fish were used in our study: Salmo trutta (brown trout) and Oncorhynchus mykiss (rainbow trout). Experiment with eggsIn order to evaluate the effect of propolis on mortality of fish eggs, approximately 600 eggs (half and half) were placed in 3 "Californian tanks" arranged in cascade. Untreated samples (about 250 eggs) were used as control. The eggs were rinsed in propolis solution (1%) for 15 min, once every four days and then they were ...

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Simulation of giant fibre development in biopsy samples from pig longissimus muscle

Cited by 24 publications

References 30 publications

New Insights into Muscle Fibre Types in Casertana Pig

New Insights into Muscle Fibre Types in Casertana Pig

The relationship of pork longissimus muscle pH to mitochondrial respiratory activities, meat quality and muscle structure

Effect of Propolis on the Fish Muscular Development and Histomorphometrical Characteristics

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