Simulation of Blood Particle Separation in a Trapezoidal Microfluidic Device by Acoustic Force

Shamloo, Amir; Parast, Farin Yazdan

doi:10.1109/ted.2018.2889912

Cited by 24 publications

(14 citation statements)

References 35 publications

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“…As an example, Shamloo et al demonstrated a numerical SSAW-based separation method of platelets, red blood cells (RBCs), and white blood cells (WBCs) by acoustic radiation force [ 82 ]. To improve the separation efficiency, the same group presented a simulation approach to separate WBCs from other blood cells in a trapezoidal channel instead of a conventional rectangular channel [ 86 ]. By optimizing the voltage and the trapezoidal leg angle, the distance from WBCs to the centerline achieved the minimum, and the more efficient separation were obtained.…”

Section: Exemplary Applications Of Acoustic Micro-object Separatiomentioning

confidence: 99%

Acoustic Microfluidic Separation Techniques and Bioapplications: A Review

Gao

Lin

et al. 2020

Micromachines

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Microfluidic separation technology has garnered significant attention over the past decade where particles are being separated at a micro/nanoscale in a rapid, low-cost, and simple manner. Amongst a myriad of separation technologies that have emerged thus far, acoustic microfluidic separation techniques are extremely apt to applications involving biological samples attributed to various advantages, including high controllability, biocompatibility, and non-invasive, label-free features. With that being said, downsides such as low throughput and dependence on external equipment still impede successful commercialization from laboratory-based prototypes. Here, we present a comprehensive review of recent advances in acoustic microfluidic separation techniques, along with exemplary applications. Specifically, an inclusive overview of fundamental theory and background is presented, then two sets of mechanisms underlying acoustic separation, bulk acoustic wave and surface acoustic wave, are introduced and discussed. Upon these summaries, we present a variety of applications based on acoustic separation. The primary focus is given to those associated with biological samples such as blood cells, cancer cells, proteins, bacteria, viruses, and DNA/RNA. Finally, we highlight the benefits and challenges behind burgeoning developments in the field and discuss the future perspectives and an outlook towards robust, integrated, and commercialized devices based on acoustic microfluidic separation.

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Section: Exemplary Applications Of Acoustic Micro-object Separatiomentioning

confidence: 99%

Acoustic Microfluidic Separation Techniques and Bioapplications: A Review

Gao

Lin

et al. 2020

Micromachines

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“…Furthermore, most of the active methods suffer from long residence time and consequently low flow rate because the cells should be sufficiently exposed to the external force field. The conventional active methods of cell separation include electrophoresis 8 , dielectrophoresis 9 , magnetophoresis 10 , acoustophoresis 11 , and optical tweezers 12 . Hybrid methods of separation can be another route for cell separation and sorting.…”

Section: Introductionmentioning

confidence: 99%

Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques

Shamloo

Naghdloo

Besanjideh

2021

Sci Rep

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Isolation of rare cancer cells is one of the important and valuable stages of cancer research. Regarding the rarity of cancer cells in blood samples, it is important to invent an efficient separation device for cell enrichment. In this study, two centrifugal microfluidic devices were designed and fabricated for the isolation of rare cancer cells. The first design (passive plan) employs a contraction–expansion array (CEA) microchannel which is connected to a bifurcation region. This device is able to isolate the target cells through inertial effects and bifurcation law. The second design (hybrid plan) also utilizes a CEA microchannel, but instead of using the bifurcation region, it is reinforced by a stack of two permanent magnets to capture the magnetically labeled target cells at the end of the microchannel. These designs were optimized by numerical simulations and tested experimentally for isolation of MCF-7 human breast cancer cells from the population of mouse fibroblast L929 cells. In order to use the hybrid design, magnetite nanoparticles were attached to the MCF-7 cells through specific Ep-CAM antibodies, and two permanent magnets of 0.34 T were utilized at the downstream of the CEA microchannel. These devices were tested at different disk rotational speeds and it was found that the passive design can isolate MCF-7 cells with a recovery rate of 76% for the rotational speed of 2100 rpm while its hybrid counterpart is able to separate the target cells with a recovery rate of 85% for the rotational speed of 1200 rpm. Although the hybrid design of separator has a better separation efficiency and higher purity, the passive one has no need for a time-consuming process of cell labeling, occupies less space on the disk, and does not impose additional costs and complexity.

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“…Microfluidics is a proven technology that has been employed to create niche solutions to biomedical applications, such as cell separation and mixing, 3D bioprinting [ 7 , 8 , 9 , 10 ], and organs-on-a-chip systems [ 11 , 12 ]. The application of microfluidic technologies can address some of the limitations of mentioned commercial cell separation methods by using different physical mechanisms, including filtration- [ 13 ], hydrodynamic- [ 14 ], inertial- [ 15 ], deterministic lateral displacement (DLD)- [ 16 , 17 ], pinched flow fractionation (PFF)- [ 18 ], centrifugation- [ 19 ], dielectrophoresis (DEP)- [ 20 ], magnetic- [ 21 ], acoustic- [ 22 ], and optical-based approaches [ 23 ]. These methods can separate target cells from a heterogeneous cell population by exploiting the differences in the properties of the cells, including their size, density, shape, deformability, and compressibility, as well as their electric, magnetic, and optical properties.…”

Section: Introductionmentioning

confidence: 99%

Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis

et al. 2020

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Separation of circulating tumor cells (CTCs) from blood samples and subsequent DNA extraction from these cells play a crucial role in cancer research and drug discovery. Microfluidics is a versatile technology that has been applied to create niche solutions to biomedical applications, such as cell separation and mixing, droplet generation, bioprinting, and organs on a chip. Centrifugal microfluidic biochips created on compact disks show great potential in processing biological samples for point of care diagnostics. This study investigates the design and numerical simulation of an integrated microfluidic device, including a cell separation unit for isolating CTCs from a blood sample and a micromixer unit for cell lysis on a rotating disk platform. For this purpose, an inertial microfluidic device was designed for the separation of target cells by using contraction–expansion microchannel arrays. Additionally, a micromixer was incorporated to mix separated target cells with the cell lysis chemical reagent to dissolve their membranes to facilitate further assays. Our numerical simulation approach was validated for both cell separation and micromixer units and corroborates existing experimental results. In the first compartment of the proposed device (cell separation unit), several simulations were performed at different angular velocities from 500 rpm to 3000 rpm to find the optimum angular velocity for maximum separation efficiency. By using the proposed inertial separation approach, CTCs, were successfully separated from white blood cells (WBCs) with high efficiency (~90%) at an angular velocity of 2000 rpm. Furthermore, a serpentine channel with rectangular obstacles was designed to achieve a highly efficient micromixer unit with high mixing quality (~98%) for isolated CTCs lysis at 2000 rpm.

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Simulation of Blood Particle Separation in a Trapezoidal Microfluidic Device by Acoustic Force

Cited by 24 publications

References 35 publications

Acoustic Microfluidic Separation Techniques and Bioapplications: A Review

Acoustic Microfluidic Separation Techniques and Bioapplications: A Review

Cancer cell enrichment on a centrifugal microfluidic platform using hydrodynamic and magnetophoretic techniques

Design and Simulation of an Integrated Centrifugal Microfluidic Device for CTCs Separation and Cell Lysis

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