Simulation for the recovery of plasmid for a DNA vaccine

Limonta, Miladys; Krajnc, Nika Lendero; Vidic, Urška; Zumalacárregui, Lourdes

doi:10.1016/j.bej.2013.09.006

Cited by 8 publications

(3 citation statements)

References 7 publications

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“…The use of simulators to model industrial processes is becoming more and more popular every day because it provides the opportunity to improve and reduce the time needed to generate different processes that integrate great applicability in different industrial branches [13]. These techniques are mainly based on the use and schematization of the process using mathematical tools and software, where all those independent and dependent variables (mass and energy balance) of the various biological and mechanical systems that make up the process are detailed to predict the natural behavior of the plant [14].…”

Section: Resultsmentioning

confidence: 99%

A Simulation Analysis of an Influenza Vaccine Production Plant in Areas of High Humanitarian Flow. A Case Study for the Region of Norte de Santander (Colombia)

Contreras-Ropero¹,

Ruiz-Roa²,

García-Martínez³

et al. 2021

Preprint

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The production of vaccines of biological origin presents a tremendous challenge for researchers. In this context, animal cell cultures are an excellent alternative for the isolation and production of biologicals against several viruses since they have an affinity with viruses and a great capacity for their replicability. Different variables have been studied to know the system's ideal parameters, allowing it to obtain profitable and competitive products. Consequently, this work focuses its efforts on evaluating an alternative for producing an anti-influenza biological from MDCK cells using SuperPro Designer v8.0 software. The process uses the DMEN culture medium supplemented with nutrients as raw material for cell development; the MDCK cells were obtained from a potential scale-up with a final working volume of 500 L, four days of residence time, inoculum volume of 10%, and continuous working mode with up to a total of 7400 h/Yr of work. The scheme has the necessary equipment for the vaccine's production, infection, and manufacture with yields of up to 416,698 units/h. In addition, it was estimated to be economically viable to produce recombinant vaccines with competitive prices of up to 0.31 USD/unit.

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Section: Resultsmentioning

confidence: 99%

A Simulation Analysis of an Influenza Vaccine Production Plant in Areas of High Humanitarian Flow. A Case Study for the Region of Norte de Santander (Colombia)

Contreras-Ropero¹,

Ruiz-Roa²,

García-Martínez³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…The use of simulators to model industrial processes is becoming more and more popular every day because it provides the opportunity to improve and reduce the time needed to generate different processes that integrate great applicability in different industrial branches [18]. These techniques are mainly based on the use and schematization of the process using mathematical tools and software, where all those independent and dependent variables (mass and energy balance) of the various biological and mechanical systems that make up the process are detailed to predict the natural behavior of the plant [19].…”

Section: Resultsmentioning

confidence: 99%

A Simulation Analysis of an Influenza Vaccine Production Plant in Areas of High Humanitarian Flow. A Preliminary Study for the Region of Norte de Santander (Colombia)

et al. 2021

View full text Add to dashboard Cite

The production of vaccines of biological origin presents a tremendous challenge for researchers. In this context, animal cell cultures are an excellent alternative for the isolation and production of biologicals against several viruses, since they have an affinity with viruses and a great capacity for their replicability. Different variables have been studied to know the system’s ideal parameters, allowing it to obtain profitable and competitive products. Consequently, this work focuses its efforts on evaluating an alternative for producing an anti-influenza biological from MDCK cells using SuperPro Designer v8.0 software. The process uses the DMEN culture medium supplemented with nutrients as raw material for cell development; the MDCK cells were obtained from a potential scale-up with a final working volume of 500 L, four days of residence time, inoculum volume of 10%, and continuous working mode with up to a total of 7400 h/Yr of work. The scheme has the necessary equipment for the vaccine’s production, infection, and manufacture with yields of up to 416,698 units/h. In addition, it was estimated to be economically viable to produce recombinant vaccines with competitive prices of up to 0.31 USD/unit.

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“…The application of simulations on industrial processes is an efficient solution for modeling and optimizing specific routes [44,45]. These techniques are based on predicting the behavior of the desired process through the calculation of the mass and energy balance of each section of the system [46].…”

Section: Discussionmentioning

confidence: 99%

A Simulation Analysis of a Microalgal-Production Plant for the Transformation of Inland-Fisheries Wastewater in Sustainable Feed

García-Martínez

Contreras-Ropero

Urbina-Suarez

et al. 2022

Water

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The present research evaluates the simulation of a system for transforming inland-fisheries wastewater into sustainable fish feed using Designer® software. The data required were obtained from the experimental cultivation of Chlorella sp. in wastewater supplemented with N and P. According to the results, it is possible to produce up to 11,875 kg/year (31.3 kg/d) with a production cost of up to 18 (USD/kg) for dry biomass and 0.19 (USD/bottle) for concentrated biomass. Similarly, it was possible to establish the kinetics of growth of substrate-dependent biomass with a maximum production of 1.25 g/L after 15 days and 98% removal of available N coupled with 20% of P. It is essential to note the final production efficiency may vary depending on uncontrollable variables such as climate and quality of wastewater, among others.

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Simulation for the recovery of plasmid for a DNA vaccine

Cited by 8 publications

References 7 publications

A Simulation Analysis of an Influenza Vaccine Production Plant in Areas of High Humanitarian Flow. A Case Study for the Region of Norte de Santander (Colombia)

A Simulation Analysis of an Influenza Vaccine Production Plant in Areas of High Humanitarian Flow. A Case Study for the Region of Norte de Santander (Colombia)

A Simulation Analysis of an Influenza Vaccine Production Plant in Areas of High Humanitarian Flow. A Preliminary Study for the Region of Norte de Santander (Colombia)

A Simulation Analysis of a Microalgal-Production Plant for the Transformation of Inland-Fisheries Wastewater in Sustainable Feed

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