Simulating the Folding Trajectories of Lattice Proteins within an Oscillatory Environment

Abstract

consequences, for example, many mutations in the genes encoding cyclic nucleotide gated (CNG) channels reflect the impaired trafficking or dysfunctionality as a spectrum of inherited retinal disorders. The effect of physical variables such as temperature had been previously analyzed, mimicking in vitro the principles encountered during in vivo folding; where protein folding and unfolding, provides thermotolerance to the cell through the heat shock proteins (Hsp) assistance, favoring the structural recovery and the function of abnormal proteins. As an alternative to rescue improper targeted membrane protein in this work, using the HEK-293 cells we overexpressed the Hsp70 isoform along with wild type CNG channel or two inheritable mutants previously reported as misfolded proteins. Comparing the effect of the proteins co-expression on cells incubated at different temperatures, monitoring the cell morphology, and channel location through fluorescence microscopy while functionality was tested by the patch clamp inside out technique.