Simulated weightlessness alters biological characteristics of human breast cancer cell line MCF-7

Qian, Airong; Zhang, Wei; Xie, Li; Weng, Yuanyuan; Yang, Pengfei; Wang, Zhe; Hu, Lifang; Xu, Huiyun; Tian, Zongcheng; Shang, Peng

doi:10.1016/j.actaastro.2008.01.024

Cited by 29 publications

(33 citation statements)

References 26 publications

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“…After exposed to MMG for 24, 48 or 72 h, U87 cells invaded and migrated more slowly than NG group, and it was in time-dependence to some degree, indicating MMG might inhibit the invasion and migration potentials of U87 cells. These results were in consistent with previous studies in other tumor cells, such as melanomas [15], human breast cancer [16] and follicular thyroid carcinoma cells [9]. Jing Li et al found that microgravity had closely associated with changing focal adhesion and cytoskeleton, and decreasing migration potential in human breast carcinoma [7].…”

Section: Discussionsupporting

confidence: 92%

Modeled microgravity suppressed invasion and migration of human glioblastoma U87 cells through downregulating store-operated calcium entry

Shi

Rao

Wang

et al. 2015

Biochemical and Biophysical Research Communications

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Section: Discussionsupporting

confidence: 92%

Modeled microgravity suppressed invasion and migration of human glioblastoma U87 cells through downregulating store-operated calcium entry

Shi

Rao

Wang

et al. 2015

Biochemical and Biophysical Research Communications

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“…This was also reported for breast cancer MCF-7 cells, in which microtubules were altered as well as the perinuclearcytokeratin network [122]. A recent paper demonstrated that clinostat-simulated microgravity affects breast cancer cell invasion, migration, adhesion, cell cycle, cell apoptosis and vinculin expression [123]. Taga et al [124] cultured murine B16-F10 melanoma cells in a rotating-wall vessel bioreactor (BIO).…”

Section: The Impact Of Weightlessness On Tumor Cellsmentioning

confidence: 77%

The Effects of Weightlessness on the Human Organism and Mammalian Cells

Pietsch¹,

Bauer²,

Egli

et al. 2011

CMM

129

110

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It has always been a desire of mankind to conquest Space. A major step in realizing this dream was the completion of the International Space Station (ISS). Living there for several months confirmed early observations of short-term spaceflights that a loss of gravity affects the health of astronauts. Space medicine tries to understand the mechanism of microgravity-induced health problems and to conceive potent countermeasures. There are four different aspects which make space medicine appealing: i) finding better strategies for adapting astronauts to weightlessness; ii) identification of microgravity-induced diseases (e.g. osteoporosis, muscle atrophy, cardiac problems and others); iii) defining new therapies to conquer these diseases which will benefit astronauts as well as people on Earth in the end; and iv) on top of that, unveiling the mechanisms of weightlessness-dependent molecular and cellular changes is a requirement for improving space medicine. In mammalian cells, microgravity induces apoptosis and alters the cytoskeleton and affects signal transduction pathways, cell differentiation, growth, proliferation, migration and adhesion. This review focused on gravi-sensitive signal transduction elements and pathways as well as molecular mechanisms in human cells, aiming to understand the cellular changes in altered gravity. Moreover, the latest information on how these changes lead to clinically relevant health problems and current strategies of countermeasures are reviewed.

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“…Most cells studied so far adjust to exposure to microgravity by altering the expression patterns of ECM and cytoskeletal proteins at gene and protein levels. [60][61][62][63][64] Preliminary indications of specific proteins involved in this process came from a comparison of the spheroid formation of the two thyroid cancer cell lines FTC-133 and CGTH-W1. The study revealed that speed of formation and size of resulting spheroids is related to the expression of certain proteins that bind fibronectin.…”

Section: Known Mechanisms Involved In Spheroid Formationmentioning

confidence: 99%

Growing Tissues in Real and Simulated Microgravity: New Methods for Tissue Engineering

Grimm

Wehland

Pietsch

et al. 2014

Tissue Engineering Part B: Reviews

121

137

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Tissue engineering in simulated (s-) and real microgravity (r-mg) is currently a topic in Space medicine contributing to biomedical sciences and their applications on Earth. The principal aim of this review is to highlight the advances and accomplishments in the field of tissue engineering that could be achieved by culturing cells in Space or by devices created to simulate microgravity on Earth. Understanding the biology of three-dimensional (3D) multicellular structures is very important for a more complete appreciation of in vivo tissue function and advancing in vitro tissue engineering efforts. Various cells exposed to r-mg in Space or to s-mg created by a random positioning machine, a 2D-clinostat, or a rotating wall vessel bioreactor grew in the form of 3D tissues. Hence, these methods represent a new strategy for tissue engineering of a variety of tissues, such as regenerated cartilage, artificial vessel constructs, and other organ tissues as well as multicellular cancer spheroids. These aggregates are used to study molecular mechanisms involved in angiogenesis, cancer development, and biology and for pharmacological testing of, for example, chemotherapeutic drugs or inhibitors of neoangiogenesis. Moreover, they are useful for studying multicellular responses in toxicology and radiation biology, or for performing coculture experiments. The future will show whether these tissue-engineered constructs can be used for medical transplantations. Unveiling the mechanisms of microgravity-dependent molecular and cellular changes is an up-to-date requirement for improving Space medicine and developing new treatment strategies that can be translated to in vivo models while reducing the use of laboratory animals.

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Simulated weightlessness alters biological characteristics of human breast cancer cell line MCF-7

Cited by 29 publications

References 26 publications

Modeled microgravity suppressed invasion and migration of human glioblastoma U87 cells through downregulating store-operated calcium entry

Modeled microgravity suppressed invasion and migration of human glioblastoma U87 cells through downregulating store-operated calcium entry

The Effects of Weightlessness on the Human Organism and Mammalian Cells

Growing Tissues in Real and Simulated Microgravity: New Methods for Tissue Engineering

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