Simulated temporary hypoxia triggers the release of CD31+/Annexin+ endothelial microparticles: A prospective pilot study in humans

Lichtenauer, Michael; Goebel, Bjoern; Fritzenwanger, Michael; Förster, Martin; Betge, Stefan; Lauten, Alexander; Figulla, Hans‐Reiner; Jung, Christian

doi:10.3233/ch-141908

Cited by 38 publications

(26 citation statements)

References 34 publications

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“…When eight healthy males were exposed to a level of hypoxia for 80 min, equivalent to that experienced at an altitude of 3000 m, they had an elevation in circulating CD106 + endothelial-cell-derived MVs [27]. Similarly, Lichtenauer et al [28] found an increase in CD31 + MVs in 14 healthy controls following a slightly different hypoxic exposure regime based on normobaric hypoxia in an air-conditioned chamber equivalent to hypoxia at 5500 m altitude. Use of CD31 positivity, in conjunction with negativity for CD41 or CD42, excludes platelet MVs, and is often used to define endothelial-cell-derived MVs, but may also comprise MVs derived from leucocytes and activated platelets.…”

Section: Hypoxiamentioning

confidence: 92%

Dynamic microvesicle release and clearance within the cardiovascular system: triggers and mechanisms

et al. 2015

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Interest in cell-derived microvesicles (or microparticles) within cardiovascular diagnostics and therapeutics is rapidly growing. Microvesicles are often measured in the circulation at a single time point. However, it is becoming clear that microvesicle levels both increase and decrease rapidly in response to certain stimuli such as hypoxia, acute cardiac stress, shear stress, hypertriglyceridaemia and inflammation. Consequently, the levels of circulating microvesicles will reflect the balance between dynamic mechanisms for release and clearance. The present review describes the range of triggers currently known to lead to microvesicle release from different cellular origins into the circulation. Specifically, the published data are used to summarize the dynamic impact of these triggers on the degree and rate of microvesicle release. Secondly, a summary of the current understanding of microvesicle clearance via different cellular systems, including the endothelial cell and macrophage, is presented, based on reported studies of clearance in experimental models and clinical scenarios, such as transfusion or cardiac stress. Together, this information can be used to provide insights into potential underlying biological mechanisms that might explain the increases or decreases in circulating microvesicle levels that have been reported and help to design future clinical studies.

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Section: Hypoxiamentioning

confidence: 92%

Dynamic microvesicle release and clearance within the cardiovascular system: triggers and mechanisms

et al. 2015

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“…In addition to pro-inflammatory agents, hypoxia has also been shown to alter the release of MPs from ECs, with highly controversial effects being reported (Vince et al, 2009; Ayers et al, 2014; Lichtenauer et al, 2015; Tuleta et al, 2015; Pichler Hefti et al, 2016). On the one hand, Lichtenauer et al and Vince et al found elevated levels of circulating AnnexinV + /CD31 + and CD106 + EMPs, respectively, in patients after exposure to temporary hypoxic conditions (Vince et al, 2009; Lichtenauer et al, 2015).…”

Section: Endothelial Extracellular Vesiclesmentioning

confidence: 99%

“…On the one hand, Lichtenauer et al and Vince et al found elevated levels of circulating AnnexinV + /CD31 + and CD106 + EMPs, respectively, in patients after exposure to temporary hypoxic conditions (Vince et al, 2009; Lichtenauer et al, 2015). On the other hand, Ayers et al did not observe significant changes in the amount of circulating CD31 + /CD41 − , CD144 + , CD62E + , and CD106 + EMPs in vivo after short-term hypoxic exposure (Ayers et al, 2014).…”

Section: Endothelial Extracellular Vesiclesmentioning

confidence: 99%

Endothelial Extracellular Vesicles—Promises and Challenges

et al. 2017

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Extracellular vesicles, including exosomes, microparticles, and apoptotic bodies, are phospholipid bilayer-enclosed vesicles that have once been considered as cell debris lacking biological functions. However, they have recently gained immense interest in the scientific community due to their role in intercellular communication, immunity, tissue regeneration as well as in the onset, and progression of various pathologic conditions. Extracellular vesicles of endothelial origin have been found to play a versatile role in the human body, since they are on the one hand known to contribute to cardiovascular diseases, but on the other hand have also been reported to promote endothelial cell survival. Hence, endothelial extracellular vesicles hold promising therapeutic potential to be used as a new tool to detect as well as treat a great number of diseases. This calls for clinically approved, standardized, and efficient isolation and characterization protocols to harvest and purify endothelial extracellular vesicles. However, such methods and techniques to fulfill stringent requirements for clinical trials have yet to be developed or are not harmonized internationally. In this review, recent advances and challenges in the field of endothelial extracellular vesicle research are discussed and current problems and limitations regarding isolation and characterization are pointed out.

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“…Obviously, an ability of apoptotic EMPs to modulate immune and inflammatory processes, coagulation and vascular function, angiogenesis and vascular injury may interact with other regulatory mechanisms the role of witch in the pathogenesis of abdominal obesity requires still being determined. It is no excluded that release of apoptotic EMPs might act as a direct endogenous survival signal for target cells (Lichtenauer et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Circulating apoptotic endothelial cell-derived microparticles are predicted metabolically unhealthy obesity

Berezin

Kremzer

Berezina³

et al. 2017

Biomed Res Ther

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Introduction: Circulating apoptotic endothelial cell-derived micro particles (EMPs) are a marker of endothelial dysfunction and cardiovascular (CV) risk in type 2 diabetes mellitus patients. There is evidence regarding association between apoptotic EMP number and CV disease in obese individuals. The aim of the study to investigate whether increased number of circulating apoptotic EMPs may predict transformation of Met-HO into Met-UHO. Methods: The study was retrospectively evolved 89 patients with established abdominal obesity (47 patients with Met-UHO determined as MetS and 42 subjects with Met-HO) from the large cohort of abdominal obesity patients (n=268). Thirty five healthy volunteers matched for age and sex were involved in the study as a control cohort. Obesity-related biomarker (adiponectin, leptin, vistafin) and EMPs were measured at baseline. Flow cytometry was used to determine EMPs with immune phenotype CD31+/ annexin V+ and CD144+/annexin V+. Results: There was not found a significant difference between numbers of EMPs labeled CD31 + / Annexin V + in Met-UHO and Met-HO patients, while Met-UHO patients had a significantly increased level of circulating CD144 + / Annexin V + compared with Met-HO individuals. Multivariate logistic regression analysis has revealed the HOMA-IR, number of CV risk factors, serum leptin and hs-CRP independently predicted numbers of circulating CD31 + / Annexin V + and CD144 + / Annexin V + EMPs in Met-UHO. In Met-HO patients HOMA-IR remained an independent predictor of increased numbers of circulating CD31 + / Annexin V + and CD144 + / Annexin V + EMPs. Conclusion: in the investigation we found that the increased number of CD31 + /Annexin V + and CD144 + / Annexin V + EMPs added to the based predictive model (HOMA-IR) may predict transformation of Met-HO into Met-UHO.

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Simulated temporary hypoxia triggers the release of CD31+/Annexin+ endothelial microparticles: A prospective pilot study in humans

Cited by 38 publications

References 34 publications

Dynamic microvesicle release and clearance within the cardiovascular system: triggers and mechanisms

Dynamic microvesicle release and clearance within the cardiovascular system: triggers and mechanisms

Endothelial Extracellular Vesicles—Promises and Challenges

Circulating apoptotic endothelial cell-derived microparticles are predicted metabolically unhealthy obesity

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