Simulated physiological stretch increases expression of extracellular matrix proteins in human bladder smooth muscle cells via integrin α4/αv-FAK-ERK1/2 signaling pathway

Chen, Shulian; Peng, Chuandu; Wei, Xin; Luo, Deyi; Lin, Yifei; Yang, Tianen; Jin, Xi; Gong, Ling; Li, Hong; Wang, Kun‐Jie

doi:10.1007/s00345-016-1993-1

Cited by 11 publications

(6 citation statements)

References 20 publications

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“…Type III collagen and decorin are located just below the basement membrane and may repress epithelial mesenchymal transition (EMT) in epithelial cells. Physiological stretching increases the expression of both genes (Kim et al 2002;Chen et al 2017). The distribution of the molecular composition of type IV collagen in the basement membrane beneath the tissue epithelium is different from tissue to tissue and is consistent with tissue-specific functions, and in bronchi the basement membrane beneath the pseudostratified columnar cells shows a pattern similar to that of skin (Saito, et al, 2000).…”

Section: Discussionmentioning

confidence: 84%

Eggshell membrane promotes homeostasis of elastic skin and lung tissue associated with type III collagen and decorin expression and ameliorates pulmonary fibrosis in a bleomycin mouse model

Ohto-Fujita

Shimizu

Atomi

et al. 2021

Preprint

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The skin and lungs are barriers to environmental threats such as toxic chemicals and microbial pathogens. The integrity of the extracellular matrix (ECM) in the dermal papillae in the skin and the interstitium in the lungs is critical for tissue homeostasis. However, it is difficult to improve the ECM integrity in the skin and lung simultaneously. Previously, we reported that eggshell membrane (ESM) provided a young ECM environment to dermal fibroblasts in vitro and in mouse skin and increased the elasticity of human skin. Herein, lung fibroblasts cultured on ESM showed markedly higher type III collagen, decorin, and MMP2 levels. Oral ESM administration in mice markedly increased the type III collagen and decorin levels in lung tissues after 2 weeks, and type III collagen, decorin, and MMP2 levels in the papillary dermis after 4 weeks. Furthermore, in a double-blind study involving 30 adults, the arm skin elasticity significantly increased after 8 weeks of ESM administration. Simultaneously, the Tiffeneau-Pinelli index, which is correlated with lung elasticity, increased also significantly. To further explore the effects of ESM on the lungs, we used a mouse model of bleomycin-induced fibrosis. In these mice, ESM significantly suppressed fibrosis at 2 weeks and increased the type III collagen levels in the bronchioles and decorin levels in the alveoli, which was implicated in the suppression of lung fibrosis. Thus, oral ESM intake may prevent the age-dependent decline of the papillary dermis and pulmonary fibrosis by improving the extracellular environment in skin and lung tissues.

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Section: Discussionmentioning

confidence: 84%

Eggshell membrane promotes homeostasis of elastic skin and lung tissue associated with type III collagen and decorin expression and ameliorates pulmonary fibrosis in a bleomycin mouse model

Ohto-Fujita

Shimizu

Atomi

et al. 2021

Preprint

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“…increased collagen deposition in the ecM is a key reason for decreased compliance (43,44). Previous studies have reported that pressure promotes an increase in ecM production around BSMcs (45,46). although the dBoo group was obstructed for a shorter period of time compared with the GBoo group and both groups were subjected to the same degree of obstruction, collagen deposition occurred earlier and more significantly in the dBoo group.…”

Section: Discussionmentioning

confidence: 98%

Urethral meatus stricture BOO stimulates bladder smooth muscle cell proliferation and pyroptosis via IL‑1β and the SGK1‑NFAT2 signaling pathway

et al. 2020

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Bladder outlet obstruction (Boo), which is primarily caused by benign prostatic hyperplasia, is a common chronic disease. However, previous studies have most commonly investigated Boo using the acute obstruction model. in the present study, a chronic obstruction model was established to investigate the different pathological alterations in the bladder between acute and chronic obstruction. compared with chronic obstruction, acute obstruction led to increased expression of proliferating cell nuclear antigen and interleukin-1β, which are markers of proliferation and inflammation, respectively. Furthermore, increased fibrosis in the bladder at week 2 was observed. Low pressure promoted mice bladder smooth muscle cell (MBSMc) proliferation, and pressure overload inhibited cell proliferation and increased the proportion of dead MBSMCs. Further investigation using serum/glucocorticoid regulated kinase 1 (SGK1) small interfering rnas indicated that low pressure may promote MBSMc proliferation by upregulating SGK1 and nuclear factor of activated T-cell expression levels. Therefore, the present study suggested that acute obstruction led to faster decompensation of bladder function and chronic bladder obstruction displayed an enhanced ability to progress to Boo.

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“…The setting of stretch elongations depends on experiment designs . It is considered that 15% elongation is a physiological stretch, while 20% elongation, or over, is a pathological one . Thus to mimic a chronic stretch on bladder smooth muscles as bladder obstruction does, 20% elongation was used in our present study.…”

Section: Discussionmentioning

confidence: 99%

Responses of bladder smooth muscle to the stretch go through extracellular signal‐regulated kinase (ERK)/p90 ribosomal S6 protein kinase (p90RSK)/Nuclear factor‐κB (NF‐κB) Pathway

Lin

et al. 2019

Neurourology and Urodynamics

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Aims The present study was designed to study changes and its potential mechanisms in human bladder smooth muscle subjected to stretch. Methods Bioinformatics analyses including differential expression analysis, overrepresentation enrichment analysis, principal component analysis, and weighted gene coexpression network analysis were used to analyze a microarray dataset (GSE47080) of partial bladder outlet obstruction (pBOO) in rat to find the potential changes of gene expressions. Bladder from pBOO model and human bladder smooth muscle cells (HBSMCs) subjected to sustained prolonged stretch were collected for Western blot analysis, real‐time polymerase chain reaction, and fluorescence analysis to verify the changes of gene expressions and preliminarily study the potential role of signaling pathway regulation in treatment of pBOO. Results The bioinformatics analysis showed that chronic obstruction activated mitogen‐activated protein kinase pathway and changed cytoskeleton structure in bladder smooth muscle. In in vivo experiments in mice, pBOO was verified by cystometry. Partial BOO activated the extracellular signal‐regulated kinase (ERK)/p90 ribosomal S6 protein kinase (p90RSK)/nuclear factor‐κB (NF‐κB) signaling pathway in DM. The messenger RNA (mRNA) expressions of contractile phenotypic proteins increased after pBOO. In in vitro experiments of HBSMCs, mechanical stretch activated ERK/p90RSK/NF‐κB in HBSMCs in a time‐dependent manner. The mRNA expressions of α‐smooth muscle actin and SM22 also increased and filamentous actin (F‐actin) polymerization was enhanced as well. Inhibition of ERK/p90RSK/NF‐κB pathway reversed mechanical stretch‐induced changes of contractile phenotypic expression and F‐action polymerization. Conclusions Continuous stretch increases expressions of contractile phenotypic proteins and promotes the polymerization of F‐actin. This process partially goes through ERK/p90RSK/NF‐κB pathway.

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Simulated physiological stretch increases expression of extracellular matrix proteins in human bladder smooth muscle cells via integrin α4/αv-FAK-ERK1/2 signaling pathway

Abstract: Simulated physiological stretch increases the expression of ECM proteins in HBSMCs, and integrin α4/αv-FAK-ERK1/2 signaling pathway partly modulates the mechano-transducing process.

Cited by 11 publications

References 20 publications

Eggshell membrane promotes homeostasis of elastic skin and lung tissue associated with type III collagen and decorin expression and ameliorates pulmonary fibrosis in a bleomycin mouse model

Eggshell membrane promotes homeostasis of elastic skin and lung tissue associated with type III collagen and decorin expression and ameliorates pulmonary fibrosis in a bleomycin mouse model

Urethral meatus stricture BOO stimulates bladder smooth muscle cell proliferation and pyroptosis via IL‑1β and the SGK1‑NFAT2 signaling pathway

Responses of bladder smooth muscle to the stretch go through extracellular signal‐regulated kinase (ERK)/p90 ribosomal S6 protein kinase (p90RSK)/Nuclear factor‐κB (NF‐κB) Pathway

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