Simulated gastrointestinal digestion of Pru ar 3 apricot allergen: Assessment of allergen resistance and characterization of the peptides by ultra‐performance liquid chromatography/electrospray ionisation mass spectrometry

Abstract

An extensive characterisation of the peptides generated from the gastrointestinal digestion of Pru ar 3 allergen was performed here for the first time via UPLC/ESI-MS analysis. The digestibility of the allergen was evaluated and compared with that of other LTPs, demonstrating that only a small amount of undigested protein remains, and that specific proteolytic action involves immunodominant epitopes. These data might explain the lower allergenicity of apricot LTP compared to peach LTP, despite their high sequence homology.

Development and validation of sensitive LC-MS/MS assays for quantification of HP-#x03B2;-CD in human plasma and CSF

“…This technique is called in-source fragmentation-or in-source collisioninduced dissociation. In-source fragmentation has been used in structural elucidation ( 14 ), compound identifi cation (15)(16)(17), compound profi ling ( 18 ), and bioanalysis ( 19 ). In-source fragmentation of HP-␤ -CD, which was promoted by increasing declustering potentials on a 4000QTRAP mass spectrometer, led to glycosidic-bond cleavage and simultaneous fragmentation of all the HP-␤ -CD …”